Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment

Detalhes bibliográficos
Autor(a) principal: Dos Santos, Danilo M.
Data de Publicação: 2022
Outros Autores: De Annunzio, Sarah R. [UNESP], Carmello, Juliana C. [UNESP], Pavarina, Ana C. [UNESP], Fontana, Carla R. [UNESP], Correa, Daniel S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1021/acsabm.1c01019
http://hdl.handle.net/11449/230113
Resumo: Periodontitis is a chronic inflammatory disease that can lead to significant destruction of tooth-supporting tissues, compromising dental function and patient's health. Although the currently employed treatment approaches can limit the advance of the disease, the development of multifunctional and hierarchically structured materials is still in demand for achieving successful tissue regeneration. Here, we combine coaxial electrospinning and 3D printing techniques to prepare bilayered zein-based membranes as a potential dual drug delivery platform for periodontal tissue regeneration. A layer of core-sheath electrospun nanofibers consisting of poly(ethylene oxide) (PEO)/curcumin (Curc)/tetracycline hydrochloride (TH) as the core and zein/poly(ϵ-caprolactone)(PCL)/β-glycerolphosphate (β-GP) as the sheath was deposited over a 3D printed honeycomb PLA/zein/Curc platform in order to render a bilayered structure that can mimic the architecture of periodontal tissue. The physicochemical properties of engineered constructs as well as the release profiles of distinct drugs were mainly controlled by varying the concentration of zein (10, 20, 30%, w/w relative to dry PCL) on the sheath layer of nanofibers, which displayed average diameters ranging from 150 to 400 nm. In vitro experiments demonstrated that the bilayered constructs provided sustained release of distinct drugs over 8 days and exhibited biocompatibility toward human oral keratinocytes (Nok-si) (cell viability >80%) as well as antibacterial activity against distinct bacterial strains including those of the red complex such as Porphyromonas gingivalis and Treponema denticola, which are recognized to elicit aggressive and chronic periodontitis. Our study reveals the potential of zein-based bilayered membranes as a dual drug delivery platform for periodontal tissue regeneration.
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spelling Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatmentasymmetric membranecontrolled releasecore-shell nanofiberselectrospinninghierarchical materialsperiodontal membranePeriodontitis is a chronic inflammatory disease that can lead to significant destruction of tooth-supporting tissues, compromising dental function and patient's health. Although the currently employed treatment approaches can limit the advance of the disease, the development of multifunctional and hierarchically structured materials is still in demand for achieving successful tissue regeneration. Here, we combine coaxial electrospinning and 3D printing techniques to prepare bilayered zein-based membranes as a potential dual drug delivery platform for periodontal tissue regeneration. A layer of core-sheath electrospun nanofibers consisting of poly(ethylene oxide) (PEO)/curcumin (Curc)/tetracycline hydrochloride (TH) as the core and zein/poly(ϵ-caprolactone)(PCL)/β-glycerolphosphate (β-GP) as the sheath was deposited over a 3D printed honeycomb PLA/zein/Curc platform in order to render a bilayered structure that can mimic the architecture of periodontal tissue. The physicochemical properties of engineered constructs as well as the release profiles of distinct drugs were mainly controlled by varying the concentration of zein (10, 20, 30%, w/w relative to dry PCL) on the sheath layer of nanofibers, which displayed average diameters ranging from 150 to 400 nm. In vitro experiments demonstrated that the bilayered constructs provided sustained release of distinct drugs over 8 days and exhibited biocompatibility toward human oral keratinocytes (Nok-si) (cell viability >80%) as well as antibacterial activity against distinct bacterial strains including those of the red complex such as Porphyromonas gingivalis and Treponema denticola, which are recognized to elicit aggressive and chronic periodontitis. Our study reveals the potential of zein-based bilayered membranes as a dual drug delivery platform for periodontal tissue regeneration.Nanotechnology National Laboratory for Agriculture (LNNA) Embrapa Instrumentação, São PauloUNESP São Paulo State University School of Pharmaceutical Sciences Department of Clinical Analysis Rodovia Araraquara Jaú, Km 01-s/n-Campos Ville, São PauloUNESP São Paulo State University School of Dentistry Department of Dental Materials and Prosthodontics, Rua Humaitá, 1680-Centro, São PauloUNESP São Paulo State University School of Pharmaceutical Sciences Department of Clinical Analysis Rodovia Araraquara Jaú, Km 01-s/n-Campos Ville, São PauloUNESP São Paulo State University School of Dentistry Department of Dental Materials and Prosthodontics, Rua Humaitá, 1680-Centro, São PauloEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (UNESP)Dos Santos, Danilo M.De Annunzio, Sarah R. [UNESP]Carmello, Juliana C. [UNESP]Pavarina, Ana C. [UNESP]Fontana, Carla R. [UNESP]Correa, Daniel S.2022-04-29T08:38:00Z2022-04-29T08:38:00Z2022-01-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article146-159http://dx.doi.org/10.1021/acsabm.1c01019ACS Applied Bio Materials, v. 5, n. 1, p. 146-159, 2022.2576-6422http://hdl.handle.net/11449/23011310.1021/acsabm.1c010192-s2.0-85121713020Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengACS Applied Bio Materialsinfo:eu-repo/semantics/openAccess2024-06-21T15:19:22Zoai:repositorio.unesp.br:11449/230113Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:46:49.409815Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment
title Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment
spellingShingle Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment
Dos Santos, Danilo M.
asymmetric membrane
controlled release
core-shell nanofibers
electrospinning
hierarchical materials
periodontal membrane
title_short Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment
title_full Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment
title_fullStr Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment
title_full_unstemmed Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment
title_sort Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment
author Dos Santos, Danilo M.
author_facet Dos Santos, Danilo M.
De Annunzio, Sarah R. [UNESP]
Carmello, Juliana C. [UNESP]
Pavarina, Ana C. [UNESP]
Fontana, Carla R. [UNESP]
Correa, Daniel S.
author_role author
author2 De Annunzio, Sarah R. [UNESP]
Carmello, Juliana C. [UNESP]
Pavarina, Ana C. [UNESP]
Fontana, Carla R. [UNESP]
Correa, Daniel S.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Dos Santos, Danilo M.
De Annunzio, Sarah R. [UNESP]
Carmello, Juliana C. [UNESP]
Pavarina, Ana C. [UNESP]
Fontana, Carla R. [UNESP]
Correa, Daniel S.
dc.subject.por.fl_str_mv asymmetric membrane
controlled release
core-shell nanofibers
electrospinning
hierarchical materials
periodontal membrane
topic asymmetric membrane
controlled release
core-shell nanofibers
electrospinning
hierarchical materials
periodontal membrane
description Periodontitis is a chronic inflammatory disease that can lead to significant destruction of tooth-supporting tissues, compromising dental function and patient's health. Although the currently employed treatment approaches can limit the advance of the disease, the development of multifunctional and hierarchically structured materials is still in demand for achieving successful tissue regeneration. Here, we combine coaxial electrospinning and 3D printing techniques to prepare bilayered zein-based membranes as a potential dual drug delivery platform for periodontal tissue regeneration. A layer of core-sheath electrospun nanofibers consisting of poly(ethylene oxide) (PEO)/curcumin (Curc)/tetracycline hydrochloride (TH) as the core and zein/poly(ϵ-caprolactone)(PCL)/β-glycerolphosphate (β-GP) as the sheath was deposited over a 3D printed honeycomb PLA/zein/Curc platform in order to render a bilayered structure that can mimic the architecture of periodontal tissue. The physicochemical properties of engineered constructs as well as the release profiles of distinct drugs were mainly controlled by varying the concentration of zein (10, 20, 30%, w/w relative to dry PCL) on the sheath layer of nanofibers, which displayed average diameters ranging from 150 to 400 nm. In vitro experiments demonstrated that the bilayered constructs provided sustained release of distinct drugs over 8 days and exhibited biocompatibility toward human oral keratinocytes (Nok-si) (cell viability >80%) as well as antibacterial activity against distinct bacterial strains including those of the red complex such as Porphyromonas gingivalis and Treponema denticola, which are recognized to elicit aggressive and chronic periodontitis. Our study reveals the potential of zein-based bilayered membranes as a dual drug delivery platform for periodontal tissue regeneration.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:38:00Z
2022-04-29T08:38:00Z
2022-01-17
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1021/acsabm.1c01019
ACS Applied Bio Materials, v. 5, n. 1, p. 146-159, 2022.
2576-6422
http://hdl.handle.net/11449/230113
10.1021/acsabm.1c01019
2-s2.0-85121713020
url http://dx.doi.org/10.1021/acsabm.1c01019
http://hdl.handle.net/11449/230113
identifier_str_mv ACS Applied Bio Materials, v. 5, n. 1, p. 146-159, 2022.
2576-6422
10.1021/acsabm.1c01019
2-s2.0-85121713020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv ACS Applied Bio Materials
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 146-159
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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