Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1021/acsabm.1c01019 http://hdl.handle.net/11449/230113 |
Resumo: | Periodontitis is a chronic inflammatory disease that can lead to significant destruction of tooth-supporting tissues, compromising dental function and patient's health. Although the currently employed treatment approaches can limit the advance of the disease, the development of multifunctional and hierarchically structured materials is still in demand for achieving successful tissue regeneration. Here, we combine coaxial electrospinning and 3D printing techniques to prepare bilayered zein-based membranes as a potential dual drug delivery platform for periodontal tissue regeneration. A layer of core-sheath electrospun nanofibers consisting of poly(ethylene oxide) (PEO)/curcumin (Curc)/tetracycline hydrochloride (TH) as the core and zein/poly(ϵ-caprolactone)(PCL)/β-glycerolphosphate (β-GP) as the sheath was deposited over a 3D printed honeycomb PLA/zein/Curc platform in order to render a bilayered structure that can mimic the architecture of periodontal tissue. The physicochemical properties of engineered constructs as well as the release profiles of distinct drugs were mainly controlled by varying the concentration of zein (10, 20, 30%, w/w relative to dry PCL) on the sheath layer of nanofibers, which displayed average diameters ranging from 150 to 400 nm. In vitro experiments demonstrated that the bilayered constructs provided sustained release of distinct drugs over 8 days and exhibited biocompatibility toward human oral keratinocytes (Nok-si) (cell viability >80%) as well as antibacterial activity against distinct bacterial strains including those of the red complex such as Porphyromonas gingivalis and Treponema denticola, which are recognized to elicit aggressive and chronic periodontitis. Our study reveals the potential of zein-based bilayered membranes as a dual drug delivery platform for periodontal tissue regeneration. |
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Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatmentasymmetric membranecontrolled releasecore-shell nanofiberselectrospinninghierarchical materialsperiodontal membranePeriodontitis is a chronic inflammatory disease that can lead to significant destruction of tooth-supporting tissues, compromising dental function and patient's health. Although the currently employed treatment approaches can limit the advance of the disease, the development of multifunctional and hierarchically structured materials is still in demand for achieving successful tissue regeneration. Here, we combine coaxial electrospinning and 3D printing techniques to prepare bilayered zein-based membranes as a potential dual drug delivery platform for periodontal tissue regeneration. A layer of core-sheath electrospun nanofibers consisting of poly(ethylene oxide) (PEO)/curcumin (Curc)/tetracycline hydrochloride (TH) as the core and zein/poly(ϵ-caprolactone)(PCL)/β-glycerolphosphate (β-GP) as the sheath was deposited over a 3D printed honeycomb PLA/zein/Curc platform in order to render a bilayered structure that can mimic the architecture of periodontal tissue. The physicochemical properties of engineered constructs as well as the release profiles of distinct drugs were mainly controlled by varying the concentration of zein (10, 20, 30%, w/w relative to dry PCL) on the sheath layer of nanofibers, which displayed average diameters ranging from 150 to 400 nm. In vitro experiments demonstrated that the bilayered constructs provided sustained release of distinct drugs over 8 days and exhibited biocompatibility toward human oral keratinocytes (Nok-si) (cell viability >80%) as well as antibacterial activity against distinct bacterial strains including those of the red complex such as Porphyromonas gingivalis and Treponema denticola, which are recognized to elicit aggressive and chronic periodontitis. Our study reveals the potential of zein-based bilayered membranes as a dual drug delivery platform for periodontal tissue regeneration.Nanotechnology National Laboratory for Agriculture (LNNA) Embrapa Instrumentação, São PauloUNESP São Paulo State University School of Pharmaceutical Sciences Department of Clinical Analysis Rodovia Araraquara Jaú, Km 01-s/n-Campos Ville, São PauloUNESP São Paulo State University School of Dentistry Department of Dental Materials and Prosthodontics, Rua Humaitá, 1680-Centro, São PauloUNESP São Paulo State University School of Pharmaceutical Sciences Department of Clinical Analysis Rodovia Araraquara Jaú, Km 01-s/n-Campos Ville, São PauloUNESP São Paulo State University School of Dentistry Department of Dental Materials and Prosthodontics, Rua Humaitá, 1680-Centro, São PauloEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (UNESP)Dos Santos, Danilo M.De Annunzio, Sarah R. [UNESP]Carmello, Juliana C. [UNESP]Pavarina, Ana C. [UNESP]Fontana, Carla R. [UNESP]Correa, Daniel S.2022-04-29T08:38:00Z2022-04-29T08:38:00Z2022-01-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article146-159http://dx.doi.org/10.1021/acsabm.1c01019ACS Applied Bio Materials, v. 5, n. 1, p. 146-159, 2022.2576-6422http://hdl.handle.net/11449/23011310.1021/acsabm.1c010192-s2.0-85121713020Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengACS Applied Bio Materialsinfo:eu-repo/semantics/openAccess2024-06-21T15:19:22Zoai:repositorio.unesp.br:11449/230113Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:46:49.409815Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment |
title |
Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment |
spellingShingle |
Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment Dos Santos, Danilo M. asymmetric membrane controlled release core-shell nanofibers electrospinning hierarchical materials periodontal membrane |
title_short |
Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment |
title_full |
Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment |
title_fullStr |
Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment |
title_full_unstemmed |
Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment |
title_sort |
Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment |
author |
Dos Santos, Danilo M. |
author_facet |
Dos Santos, Danilo M. De Annunzio, Sarah R. [UNESP] Carmello, Juliana C. [UNESP] Pavarina, Ana C. [UNESP] Fontana, Carla R. [UNESP] Correa, Daniel S. |
author_role |
author |
author2 |
De Annunzio, Sarah R. [UNESP] Carmello, Juliana C. [UNESP] Pavarina, Ana C. [UNESP] Fontana, Carla R. [UNESP] Correa, Daniel S. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Dos Santos, Danilo M. De Annunzio, Sarah R. [UNESP] Carmello, Juliana C. [UNESP] Pavarina, Ana C. [UNESP] Fontana, Carla R. [UNESP] Correa, Daniel S. |
dc.subject.por.fl_str_mv |
asymmetric membrane controlled release core-shell nanofibers electrospinning hierarchical materials periodontal membrane |
topic |
asymmetric membrane controlled release core-shell nanofibers electrospinning hierarchical materials periodontal membrane |
description |
Periodontitis is a chronic inflammatory disease that can lead to significant destruction of tooth-supporting tissues, compromising dental function and patient's health. Although the currently employed treatment approaches can limit the advance of the disease, the development of multifunctional and hierarchically structured materials is still in demand for achieving successful tissue regeneration. Here, we combine coaxial electrospinning and 3D printing techniques to prepare bilayered zein-based membranes as a potential dual drug delivery platform for periodontal tissue regeneration. A layer of core-sheath electrospun nanofibers consisting of poly(ethylene oxide) (PEO)/curcumin (Curc)/tetracycline hydrochloride (TH) as the core and zein/poly(ϵ-caprolactone)(PCL)/β-glycerolphosphate (β-GP) as the sheath was deposited over a 3D printed honeycomb PLA/zein/Curc platform in order to render a bilayered structure that can mimic the architecture of periodontal tissue. The physicochemical properties of engineered constructs as well as the release profiles of distinct drugs were mainly controlled by varying the concentration of zein (10, 20, 30%, w/w relative to dry PCL) on the sheath layer of nanofibers, which displayed average diameters ranging from 150 to 400 nm. In vitro experiments demonstrated that the bilayered constructs provided sustained release of distinct drugs over 8 days and exhibited biocompatibility toward human oral keratinocytes (Nok-si) (cell viability >80%) as well as antibacterial activity against distinct bacterial strains including those of the red complex such as Porphyromonas gingivalis and Treponema denticola, which are recognized to elicit aggressive and chronic periodontitis. Our study reveals the potential of zein-based bilayered membranes as a dual drug delivery platform for periodontal tissue regeneration. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:38:00Z 2022-04-29T08:38:00Z 2022-01-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1021/acsabm.1c01019 ACS Applied Bio Materials, v. 5, n. 1, p. 146-159, 2022. 2576-6422 http://hdl.handle.net/11449/230113 10.1021/acsabm.1c01019 2-s2.0-85121713020 |
url |
http://dx.doi.org/10.1021/acsabm.1c01019 http://hdl.handle.net/11449/230113 |
identifier_str_mv |
ACS Applied Bio Materials, v. 5, n. 1, p. 146-159, 2022. 2576-6422 10.1021/acsabm.1c01019 2-s2.0-85121713020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
ACS Applied Bio Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
146-159 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129356898238464 |