Osteocalcin immunolabeling during the alveolar healing process in ovariectomized rats treated with estrogen or raloxifene
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bone.2009.12.016 http://hdl.handle.net/11449/14964 |
Resumo: | The influence of an estrogen-deficient state was evaluated in this study and also its treatments with estrogen (E(2)) or with raloxifene (RLX) on the expression of osteocalcin during the periods of the chronology of the alveolar bone healing process (7, 14, 21, 28 and 42 post-extraction days) by means of immunohistochemistry reactions and histomorphometric analysis. Rats (200-220 g) with oestrus cycles normal were either OVX or sham-operated and divided into four groups: sham, OVX control (OVX/O), estrogen (OVX/E(2); 17 beta-estradiol, 400 mu g/mo) and raloxifene (OVX/RLX; 1 mg/kg bw/d) groups. Histomorphometric analysis showed the sham group presented the highest mean value of bone formation post-extraction. The reaction of immunohistochemistry for osteocalcin presented stronger expression of osteocalcin with predominance at 14 and 21 days on sham group. The OVX/RLX group presented better results than OVX/E2, considering the expression of osteocalcin in osteoblastic lineage cells, but still inferior than the sham group. It was concluded that ovariectomy decreases the mineralization process and the osteocalcin expression during the chronology of the alveolar healing process that is not totally recovered with estrogen replacement or raloxifene treatment. (C) 2009 Elsevier B.V. All rights reserved. |
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Osteocalcin immunolabeling during the alveolar healing process in ovariectomized rats treated with estrogen or raloxifeneAlveolar processOsteocalcinOvariectomyRaloxifeneEstradiolThe influence of an estrogen-deficient state was evaluated in this study and also its treatments with estrogen (E(2)) or with raloxifene (RLX) on the expression of osteocalcin during the periods of the chronology of the alveolar bone healing process (7, 14, 21, 28 and 42 post-extraction days) by means of immunohistochemistry reactions and histomorphometric analysis. Rats (200-220 g) with oestrus cycles normal were either OVX or sham-operated and divided into four groups: sham, OVX control (OVX/O), estrogen (OVX/E(2); 17 beta-estradiol, 400 mu g/mo) and raloxifene (OVX/RLX; 1 mg/kg bw/d) groups. Histomorphometric analysis showed the sham group presented the highest mean value of bone formation post-extraction. The reaction of immunohistochemistry for osteocalcin presented stronger expression of osteocalcin with predominance at 14 and 21 days on sham group. The OVX/RLX group presented better results than OVX/E2, considering the expression of osteocalcin in osteoblastic lineage cells, but still inferior than the sham group. It was concluded that ovariectomy decreases the mineralization process and the osteocalcin expression during the chronology of the alveolar healing process that is not totally recovered with estrogen replacement or raloxifene treatment. (C) 2009 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, UNESP, Dept Surg, São Paulo, BrazilUniv Estadual Paulista, UNESP, Integrated Clin, São Paulo, BrazilUniv Estadual Paulista, UNESP, Dent Sch Aracatuba, Dept Basic Sci, São Paulo, BrazilUniv Estadual Paulista, UNESP, Dept Surg, São Paulo, BrazilUniv Estadual Paulista, UNESP, Integrated Clin, São Paulo, BrazilUniv Estadual Paulista, UNESP, Dent Sch Aracatuba, Dept Basic Sci, São Paulo, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Luvizuto, Eloa Rodrigues [UNESP]Damasio Dias, Sheila Monica [UNESP]Queiroz, Thallita Pereira [UNESP]Okamoto, Tetuo [UNESP]Garcia, Idelmo Rangel [UNESP]Okamoto, Roberta [UNESP]Dornelles, Rita Cassia Menegati [UNESP]2013-09-30T18:29:10Z2014-05-20T13:43:00Z2013-09-30T18:29:10Z2014-05-20T13:43:00Z2010-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject1021-1029http://dx.doi.org/10.1016/j.bone.2009.12.016Bone. New York: Elsevier B.V., v. 46, n. 4, p. 1021-1029, 2010.8756-3282http://hdl.handle.net/11449/1496410.1016/j.bone.2009.12.016WOS:0002760094000220169955363968097156692821982805654359024227848890000-0003-0783-6612Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBone4.4551,652info:eu-repo/semantics/openAccess2021-10-23T21:44:14Zoai:repositorio.unesp.br:11449/14964Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:56:56.421827Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Osteocalcin immunolabeling during the alveolar healing process in ovariectomized rats treated with estrogen or raloxifene |
title |
Osteocalcin immunolabeling during the alveolar healing process in ovariectomized rats treated with estrogen or raloxifene |
spellingShingle |
Osteocalcin immunolabeling during the alveolar healing process in ovariectomized rats treated with estrogen or raloxifene Luvizuto, Eloa Rodrigues [UNESP] Alveolar process Osteocalcin Ovariectomy Raloxifene Estradiol |
title_short |
Osteocalcin immunolabeling during the alveolar healing process in ovariectomized rats treated with estrogen or raloxifene |
title_full |
Osteocalcin immunolabeling during the alveolar healing process in ovariectomized rats treated with estrogen or raloxifene |
title_fullStr |
Osteocalcin immunolabeling during the alveolar healing process in ovariectomized rats treated with estrogen or raloxifene |
title_full_unstemmed |
Osteocalcin immunolabeling during the alveolar healing process in ovariectomized rats treated with estrogen or raloxifene |
title_sort |
Osteocalcin immunolabeling during the alveolar healing process in ovariectomized rats treated with estrogen or raloxifene |
author |
Luvizuto, Eloa Rodrigues [UNESP] |
author_facet |
Luvizuto, Eloa Rodrigues [UNESP] Damasio Dias, Sheila Monica [UNESP] Queiroz, Thallita Pereira [UNESP] Okamoto, Tetuo [UNESP] Garcia, Idelmo Rangel [UNESP] Okamoto, Roberta [UNESP] Dornelles, Rita Cassia Menegati [UNESP] |
author_role |
author |
author2 |
Damasio Dias, Sheila Monica [UNESP] Queiroz, Thallita Pereira [UNESP] Okamoto, Tetuo [UNESP] Garcia, Idelmo Rangel [UNESP] Okamoto, Roberta [UNESP] Dornelles, Rita Cassia Menegati [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Luvizuto, Eloa Rodrigues [UNESP] Damasio Dias, Sheila Monica [UNESP] Queiroz, Thallita Pereira [UNESP] Okamoto, Tetuo [UNESP] Garcia, Idelmo Rangel [UNESP] Okamoto, Roberta [UNESP] Dornelles, Rita Cassia Menegati [UNESP] |
dc.subject.por.fl_str_mv |
Alveolar process Osteocalcin Ovariectomy Raloxifene Estradiol |
topic |
Alveolar process Osteocalcin Ovariectomy Raloxifene Estradiol |
description |
The influence of an estrogen-deficient state was evaluated in this study and also its treatments with estrogen (E(2)) or with raloxifene (RLX) on the expression of osteocalcin during the periods of the chronology of the alveolar bone healing process (7, 14, 21, 28 and 42 post-extraction days) by means of immunohistochemistry reactions and histomorphometric analysis. Rats (200-220 g) with oestrus cycles normal were either OVX or sham-operated and divided into four groups: sham, OVX control (OVX/O), estrogen (OVX/E(2); 17 beta-estradiol, 400 mu g/mo) and raloxifene (OVX/RLX; 1 mg/kg bw/d) groups. Histomorphometric analysis showed the sham group presented the highest mean value of bone formation post-extraction. The reaction of immunohistochemistry for osteocalcin presented stronger expression of osteocalcin with predominance at 14 and 21 days on sham group. The OVX/RLX group presented better results than OVX/E2, considering the expression of osteocalcin in osteoblastic lineage cells, but still inferior than the sham group. It was concluded that ovariectomy decreases the mineralization process and the osteocalcin expression during the chronology of the alveolar healing process that is not totally recovered with estrogen replacement or raloxifene treatment. (C) 2009 Elsevier B.V. All rights reserved. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-04-01 2013-09-30T18:29:10Z 2013-09-30T18:29:10Z 2014-05-20T13:43:00Z 2014-05-20T13:43:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bone.2009.12.016 Bone. New York: Elsevier B.V., v. 46, n. 4, p. 1021-1029, 2010. 8756-3282 http://hdl.handle.net/11449/14964 10.1016/j.bone.2009.12.016 WOS:000276009400022 0169955363968097 1566928219828056 5435902422784889 0000-0003-0783-6612 |
url |
http://dx.doi.org/10.1016/j.bone.2009.12.016 http://hdl.handle.net/11449/14964 |
identifier_str_mv |
Bone. New York: Elsevier B.V., v. 46, n. 4, p. 1021-1029, 2010. 8756-3282 10.1016/j.bone.2009.12.016 WOS:000276009400022 0169955363968097 1566928219828056 5435902422784889 0000-0003-0783-6612 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bone 4.455 1,652 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1021-1029 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129265188732928 |