In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins

Detalhes bibliográficos
Autor(a) principal: Srimahaeak, Thanyaporn
Data de Publicação: 2021
Outros Autores: Bianchi, Fernanda [UNESP], Chlumsky, Ondrej, Larsen, Nadja, Jespersen, Lene
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jff.2021.104395
http://hdl.handle.net/11449/206052
Resumo: This study investigated the effect of four citrus pectins on adhesion of the probiotic Limosilactobacillus fermentum PCC and integrity of the intestinal epithelial monolayers using the Caco-2 cell model. Adhesion of L. fermentum PCC was enhanced (from 35% to 54–73%) in the presence of pectins with high and moderate degree of esterification (DE), whereas it was reduced (to 2%) by a pectin with low DE. Transepithelial electrical resistance (TEER) was significantly increased upon exposure to L. fermentum PCC alone (by 33%) and, to a greater extent, by combinations with pectins (by 44 – 48%), suggesting synergistic action of bacteria and pectins. Increases in TEER were related to transcriptional responses of the tight junction genes, encoding claudin-4 and claudin-2 proteins. The overall results indicated that pectins, when applied in synbiotic combination with L. fermentum PCC, have potential to improve bacterial adhesion and the intestinal epithelial barrier.
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spelling In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectinsAdhesionCaco-2 cellsLimosiLactobacillus fermentum PCCPectinsTight junctionThis study investigated the effect of four citrus pectins on adhesion of the probiotic Limosilactobacillus fermentum PCC and integrity of the intestinal epithelial monolayers using the Caco-2 cell model. Adhesion of L. fermentum PCC was enhanced (from 35% to 54–73%) in the presence of pectins with high and moderate degree of esterification (DE), whereas it was reduced (to 2%) by a pectin with low DE. Transepithelial electrical resistance (TEER) was significantly increased upon exposure to L. fermentum PCC alone (by 33%) and, to a greater extent, by combinations with pectins (by 44 – 48%), suggesting synergistic action of bacteria and pectins. Increases in TEER were related to transcriptional responses of the tight junction genes, encoding claudin-4 and claudin-2 proteins. The overall results indicated that pectins, when applied in synbiotic combination with L. fermentum PCC, have potential to improve bacterial adhesion and the intestinal epithelial barrier.Department of Food Science Faculty of Science University of CopenhagenDepartment of Food Science UNESP - São Paulo State UniversityDepartment of Experimental Biology Masaryk UniversityDepartment of Food Science UNESP - São Paulo State UniversityUniversity of CopenhagenUniversidade Estadual Paulista (Unesp)Masaryk UniversitySrimahaeak, ThanyapornBianchi, Fernanda [UNESP]Chlumsky, OndrejLarsen, NadjaJespersen, Lene2021-06-25T10:25:45Z2021-06-25T10:25:45Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jff.2021.104395Journal of Functional Foods, v. 79.1756-4646http://hdl.handle.net/11449/20605210.1016/j.jff.2021.1043952-s2.0-85102607583Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Functional Foodsinfo:eu-repo/semantics/openAccess2021-10-22T20:42:55Zoai:repositorio.unesp.br:11449/206052Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:48:40.698196Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins
title In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins
spellingShingle In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins
Srimahaeak, Thanyaporn
Adhesion
Caco-2 cells
LimosiLactobacillus fermentum PCC
Pectins
Tight junction
title_short In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins
title_full In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins
title_fullStr In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins
title_full_unstemmed In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins
title_sort In-vitro study of Limosilactobacillus fermentum PCC adhesion to and integrity of the Caco-2 cell monolayers as affected by pectins
author Srimahaeak, Thanyaporn
author_facet Srimahaeak, Thanyaporn
Bianchi, Fernanda [UNESP]
Chlumsky, Ondrej
Larsen, Nadja
Jespersen, Lene
author_role author
author2 Bianchi, Fernanda [UNESP]
Chlumsky, Ondrej
Larsen, Nadja
Jespersen, Lene
author2_role author
author
author
author
dc.contributor.none.fl_str_mv University of Copenhagen
Universidade Estadual Paulista (Unesp)
Masaryk University
dc.contributor.author.fl_str_mv Srimahaeak, Thanyaporn
Bianchi, Fernanda [UNESP]
Chlumsky, Ondrej
Larsen, Nadja
Jespersen, Lene
dc.subject.por.fl_str_mv Adhesion
Caco-2 cells
LimosiLactobacillus fermentum PCC
Pectins
Tight junction
topic Adhesion
Caco-2 cells
LimosiLactobacillus fermentum PCC
Pectins
Tight junction
description This study investigated the effect of four citrus pectins on adhesion of the probiotic Limosilactobacillus fermentum PCC and integrity of the intestinal epithelial monolayers using the Caco-2 cell model. Adhesion of L. fermentum PCC was enhanced (from 35% to 54–73%) in the presence of pectins with high and moderate degree of esterification (DE), whereas it was reduced (to 2%) by a pectin with low DE. Transepithelial electrical resistance (TEER) was significantly increased upon exposure to L. fermentum PCC alone (by 33%) and, to a greater extent, by combinations with pectins (by 44 – 48%), suggesting synergistic action of bacteria and pectins. Increases in TEER were related to transcriptional responses of the tight junction genes, encoding claudin-4 and claudin-2 proteins. The overall results indicated that pectins, when applied in synbiotic combination with L. fermentum PCC, have potential to improve bacterial adhesion and the intestinal epithelial barrier.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:25:45Z
2021-06-25T10:25:45Z
2021-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jff.2021.104395
Journal of Functional Foods, v. 79.
1756-4646
http://hdl.handle.net/11449/206052
10.1016/j.jff.2021.104395
2-s2.0-85102607583
url http://dx.doi.org/10.1016/j.jff.2021.104395
http://hdl.handle.net/11449/206052
identifier_str_mv Journal of Functional Foods, v. 79.
1756-4646
10.1016/j.jff.2021.104395
2-s2.0-85102607583
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Functional Foods
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129120748437504

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