Ureaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidase
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11033-022-08183-6 http://hdl.handle.net/11449/248230 |
Resumo: | Background: Ureaplasma, a genus of the order Mycoplasmatales and commonly grouped with Mycoplasma as genital mycoplasma is one of the most common microbes isolated from women with infection/inflammation-associated preterm labor (PTL). Mycoplasma spp. produce sialidase that cleaves sialic acid from glycans of vaginal mucous membranes and facilitates adherence and invasion of the epithelium by pathobionts, and dysregulated immune response. However, whether Ureaplasma species can induce the production of sialidase is yet to be demonstrated. We examined U. parvum-infected vaginal epithelial cells (VECs) for the production of sialidase and pro-inflammatory cytokines. Methods: Immortalized VECs were cultured in appropriate media and treated with U. parvum in a concentration of 1 × 105 DNA copies/ml. After 24 h of treatment, cells and media were harvested. To confirm infection and cell uptake, immunocytochemistry for multi-banded antigen (MBA) was performed. Pro-inflammatory cytokine production and protein analysis for sialidase confirmed pro-labor pathways. Results: Infection of VECs was confirmed by the presence of intracellular MBA. Western blot analysis showed no significant increase in sialidase expression from U. parvum-treated VECs compared to uninfected cells. However, U. parvum infection induced 2-3-fold increased production of GM-CSF (p = 0.03), IL-6 (p = 0.01), and IL-8 (p = 0.01) in VECs compared to controls. Conclusion: U. parvum infection of VECs induced inflammatory imbalance associated with vaginal dysbiosis but did not alter sialidase expression at the cellular level. These data suggest that U. parvum’s pathogenic effect could be propagated by locally produced pro-inflammatory cytokines and, unlike other genital mycoplasmas, may be independent of sialidase. |
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Ureaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidaseCytokinesInflammationPreterm birthSialidaseUreaplasma parvumVaginal epithelial cellsBackground: Ureaplasma, a genus of the order Mycoplasmatales and commonly grouped with Mycoplasma as genital mycoplasma is one of the most common microbes isolated from women with infection/inflammation-associated preterm labor (PTL). Mycoplasma spp. produce sialidase that cleaves sialic acid from glycans of vaginal mucous membranes and facilitates adherence and invasion of the epithelium by pathobionts, and dysregulated immune response. However, whether Ureaplasma species can induce the production of sialidase is yet to be demonstrated. We examined U. parvum-infected vaginal epithelial cells (VECs) for the production of sialidase and pro-inflammatory cytokines. Methods: Immortalized VECs were cultured in appropriate media and treated with U. parvum in a concentration of 1 × 105 DNA copies/ml. After 24 h of treatment, cells and media were harvested. To confirm infection and cell uptake, immunocytochemistry for multi-banded antigen (MBA) was performed. Pro-inflammatory cytokine production and protein analysis for sialidase confirmed pro-labor pathways. Results: Infection of VECs was confirmed by the presence of intracellular MBA. Western blot analysis showed no significant increase in sialidase expression from U. parvum-treated VECs compared to uninfected cells. However, U. parvum infection induced 2-3-fold increased production of GM-CSF (p = 0.03), IL-6 (p = 0.01), and IL-8 (p = 0.01) in VECs compared to controls. Conclusion: U. parvum infection of VECs induced inflammatory imbalance associated with vaginal dysbiosis but did not alter sialidase expression at the cellular level. These data suggest that U. parvum’s pathogenic effect could be propagated by locally produced pro-inflammatory cytokines and, unlike other genital mycoplasmas, may be independent of sialidase.Department of Oncology and Metabolism University of SheffieldDepartment of Obstetrics and Gynecology The University of Texas Medical Branch at GalvestonDepartment of Pathology Universidade Estadual Paulista Botucatu Medical SchoolAcademic Unit of Reproductive and Developmental Medicine Department of Oncology and Metabolism The University of Sheffield, 4th Floor, Jessop Wing, Tree Root WalkDepartment of Obstetrics & Gynecology The University of Texas Medical Branch at Galveston, 301 University BlvdDepartment of Pathology Universidade Estadual Paulista Botucatu Medical SchoolUniversity of SheffieldThe University of Texas Medical Branch at GalvestonUniversidade Estadual Paulista (UNESP)The University of SheffieldAmabebe, EmmanuelRichardson, Lauren S.Bento, Giovana Fernanda Cosi [UNESP]Radnaa, EnkhtuyaKechichian, TalarMenon, RamkumarAnumba, Dilly O. C.2023-07-29T13:38:10Z2023-07-29T13:38:10Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3035-3043http://dx.doi.org/10.1007/s11033-022-08183-6Molecular Biology Reports, v. 50, n. 4, p. 3035-3043, 2023.1573-49780301-4851http://hdl.handle.net/11449/24823010.1007/s11033-022-08183-62-s2.0-85146546255Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular Biology Reportsinfo:eu-repo/semantics/openAccess2023-07-29T13:38:10Zoai:repositorio.unesp.br:11449/248230Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:38:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ureaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidase |
title |
Ureaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidase |
spellingShingle |
Ureaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidase Amabebe, Emmanuel Cytokines Inflammation Preterm birth Sialidase Ureaplasma parvum Vaginal epithelial cells |
title_short |
Ureaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidase |
title_full |
Ureaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidase |
title_fullStr |
Ureaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidase |
title_full_unstemmed |
Ureaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidase |
title_sort |
Ureaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidase |
author |
Amabebe, Emmanuel |
author_facet |
Amabebe, Emmanuel Richardson, Lauren S. Bento, Giovana Fernanda Cosi [UNESP] Radnaa, Enkhtuya Kechichian, Talar Menon, Ramkumar Anumba, Dilly O. C. |
author_role |
author |
author2 |
Richardson, Lauren S. Bento, Giovana Fernanda Cosi [UNESP] Radnaa, Enkhtuya Kechichian, Talar Menon, Ramkumar Anumba, Dilly O. C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
University of Sheffield The University of Texas Medical Branch at Galveston Universidade Estadual Paulista (UNESP) The University of Sheffield |
dc.contributor.author.fl_str_mv |
Amabebe, Emmanuel Richardson, Lauren S. Bento, Giovana Fernanda Cosi [UNESP] Radnaa, Enkhtuya Kechichian, Talar Menon, Ramkumar Anumba, Dilly O. C. |
dc.subject.por.fl_str_mv |
Cytokines Inflammation Preterm birth Sialidase Ureaplasma parvum Vaginal epithelial cells |
topic |
Cytokines Inflammation Preterm birth Sialidase Ureaplasma parvum Vaginal epithelial cells |
description |
Background: Ureaplasma, a genus of the order Mycoplasmatales and commonly grouped with Mycoplasma as genital mycoplasma is one of the most common microbes isolated from women with infection/inflammation-associated preterm labor (PTL). Mycoplasma spp. produce sialidase that cleaves sialic acid from glycans of vaginal mucous membranes and facilitates adherence and invasion of the epithelium by pathobionts, and dysregulated immune response. However, whether Ureaplasma species can induce the production of sialidase is yet to be demonstrated. We examined U. parvum-infected vaginal epithelial cells (VECs) for the production of sialidase and pro-inflammatory cytokines. Methods: Immortalized VECs were cultured in appropriate media and treated with U. parvum in a concentration of 1 × 105 DNA copies/ml. After 24 h of treatment, cells and media were harvested. To confirm infection and cell uptake, immunocytochemistry for multi-banded antigen (MBA) was performed. Pro-inflammatory cytokine production and protein analysis for sialidase confirmed pro-labor pathways. Results: Infection of VECs was confirmed by the presence of intracellular MBA. Western blot analysis showed no significant increase in sialidase expression from U. parvum-treated VECs compared to uninfected cells. However, U. parvum infection induced 2-3-fold increased production of GM-CSF (p = 0.03), IL-6 (p = 0.01), and IL-8 (p = 0.01) in VECs compared to controls. Conclusion: U. parvum infection of VECs induced inflammatory imbalance associated with vaginal dysbiosis but did not alter sialidase expression at the cellular level. These data suggest that U. parvum’s pathogenic effect could be propagated by locally produced pro-inflammatory cytokines and, unlike other genital mycoplasmas, may be independent of sialidase. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:38:10Z 2023-07-29T13:38:10Z 2023-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11033-022-08183-6 Molecular Biology Reports, v. 50, n. 4, p. 3035-3043, 2023. 1573-4978 0301-4851 http://hdl.handle.net/11449/248230 10.1007/s11033-022-08183-6 2-s2.0-85146546255 |
url |
http://dx.doi.org/10.1007/s11033-022-08183-6 http://hdl.handle.net/11449/248230 |
identifier_str_mv |
Molecular Biology Reports, v. 50, n. 4, p. 3035-3043, 2023. 1573-4978 0301-4851 10.1007/s11033-022-08183-6 2-s2.0-85146546255 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular Biology Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3035-3043 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1803649747758612480 |