Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2013/431465 http://hdl.handle.net/11449/112509 |
Resumo: | Streptococcus mutans, the predominant bacterial species associated with dental caries, can enter the bloodstream and cause infective endocarditis. The aim of this study was to investigate S. mutans biofilm formation and adherence to endothelial cells induced by human fibrinogen. The putative mechanism by which biofilm formation is induced as well as the impact of fibrinogen on S. mutans resistance to penicillin was also evaluated. Bovine plasma dose dependently induced biofilm formation by S. mutans. Of the various plasma proteins tested, only fibrinogen promoted the formation of biofilm in a dose-dependent manner. Scanning electron microscopy observations revealed the presence of complex aggregates of bacterial cells firmly attached to the polystyrene support. S. mutans in biofilms induced by the presence of fibrinogen was markedly resistant to the bactericidal effect of penicillin. Fibrinogen also significantly increased the adherence of S. mutans to endothelial cells. Neither S. mutans cells nor culture supernatants converted fibrinogen into fibrin. However, fibrinogen is specifically bound to the cell surface of S. mutans and may act as a bridging molecule to mediate biofilm formation. In conclusion, our study identified a new mechanism promoting S. mutans biofilm formation and adherence to endothelial cells which may contribute to infective endocarditis. |
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Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial CellsStreptococcus mutans, the predominant bacterial species associated with dental caries, can enter the bloodstream and cause infective endocarditis. The aim of this study was to investigate S. mutans biofilm formation and adherence to endothelial cells induced by human fibrinogen. The putative mechanism by which biofilm formation is induced as well as the impact of fibrinogen on S. mutans resistance to penicillin was also evaluated. Bovine plasma dose dependently induced biofilm formation by S. mutans. Of the various plasma proteins tested, only fibrinogen promoted the formation of biofilm in a dose-dependent manner. Scanning electron microscopy observations revealed the presence of complex aggregates of bacterial cells firmly attached to the polystyrene support. S. mutans in biofilms induced by the presence of fibrinogen was markedly resistant to the bactericidal effect of penicillin. Fibrinogen also significantly increased the adherence of S. mutans to endothelial cells. Neither S. mutans cells nor culture supernatants converted fibrinogen into fibrin. However, fibrinogen is specifically bound to the cell surface of S. mutans and may act as a bridging molecule to mediate biofilm formation. In conclusion, our study identified a new mechanism promoting S. mutans biofilm formation and adherence to endothelial cells which may contribute to infective endocarditis.Canadian Institutes of Health ResearchState Univ Sao Paulo, Araraquara Dent Sch, Dept Oral Diag & Surg, BR-14801903 Araraquara, SP, BrazilUniv Laval, Oral Ecol Res Grp, Fac Dent, Quebec City, PQ G1V 0A6, CanadaState Univ Sao Paulo, Dept Physiol & Pathol, Araraquara Dent Sch, BR-14801903 Araraquara, SP, BrazilState Univ Sao Paulo, Araraquara Dent Sch, Dept Oral Diag & Surg, BR-14801903 Araraquara, SP, BrazilState Univ Sao Paulo, Dept Physiol & Pathol, Araraquara Dent Sch, BR-14801903 Araraquara, SP, BrazilHindawi Publishing CorporationUniversidade Estadual Paulista (Unesp)Univ LavalLombardo Bedran, Telma Blanca [UNESP]Azelmat, JabraneSpolidorio, Denise Palomari [UNESP]Grenier, Daniel2014-12-03T13:10:46Z2014-12-03T13:10:46Z2013-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1155/2013/431465Biomed Research International. New York: Hindawi Publishing Corporation, 8 p., 2013.2314-6133http://hdl.handle.net/11449/11250910.1155/2013/431465WOS:000325878700001WOS000325878700001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioMed Research International2.5830,935info:eu-repo/semantics/openAccess2023-12-25T06:21:55Zoai:repositorio.unesp.br:11449/112509Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-25T06:21:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells |
title |
Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells |
spellingShingle |
Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells Lombardo Bedran, Telma Blanca [UNESP] |
title_short |
Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells |
title_full |
Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells |
title_fullStr |
Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells |
title_full_unstemmed |
Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells |
title_sort |
Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells |
author |
Lombardo Bedran, Telma Blanca [UNESP] |
author_facet |
Lombardo Bedran, Telma Blanca [UNESP] Azelmat, Jabrane Spolidorio, Denise Palomari [UNESP] Grenier, Daniel |
author_role |
author |
author2 |
Azelmat, Jabrane Spolidorio, Denise Palomari [UNESP] Grenier, Daniel |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Laval |
dc.contributor.author.fl_str_mv |
Lombardo Bedran, Telma Blanca [UNESP] Azelmat, Jabrane Spolidorio, Denise Palomari [UNESP] Grenier, Daniel |
description |
Streptococcus mutans, the predominant bacterial species associated with dental caries, can enter the bloodstream and cause infective endocarditis. The aim of this study was to investigate S. mutans biofilm formation and adherence to endothelial cells induced by human fibrinogen. The putative mechanism by which biofilm formation is induced as well as the impact of fibrinogen on S. mutans resistance to penicillin was also evaluated. Bovine plasma dose dependently induced biofilm formation by S. mutans. Of the various plasma proteins tested, only fibrinogen promoted the formation of biofilm in a dose-dependent manner. Scanning electron microscopy observations revealed the presence of complex aggregates of bacterial cells firmly attached to the polystyrene support. S. mutans in biofilms induced by the presence of fibrinogen was markedly resistant to the bactericidal effect of penicillin. Fibrinogen also significantly increased the adherence of S. mutans to endothelial cells. Neither S. mutans cells nor culture supernatants converted fibrinogen into fibrin. However, fibrinogen is specifically bound to the cell surface of S. mutans and may act as a bridging molecule to mediate biofilm formation. In conclusion, our study identified a new mechanism promoting S. mutans biofilm formation and adherence to endothelial cells which may contribute to infective endocarditis. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 2014-12-03T13:10:46Z 2014-12-03T13:10:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2013/431465 Biomed Research International. New York: Hindawi Publishing Corporation, 8 p., 2013. 2314-6133 http://hdl.handle.net/11449/112509 10.1155/2013/431465 WOS:000325878700001 WOS000325878700001.pdf |
url |
http://dx.doi.org/10.1155/2013/431465 http://hdl.handle.net/11449/112509 |
identifier_str_mv |
Biomed Research International. New York: Hindawi Publishing Corporation, 8 p., 2013. 2314-6133 10.1155/2013/431465 WOS:000325878700001 WOS000325878700001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BioMed Research International 2.583 0,935 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650080814661632 |