Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom

Detalhes bibliográficos
Autor(a) principal: Ullah, Anwar [UNESP]
Data de Publicação: 2011
Outros Autores: de Giuseppe, Priscila Oliveira, Murakami, Mario Tyago, Trevisan-Silva, Dilza, Martins Wille, Ana Carolina, Chaves-Moreira, Daniele, Gremski, Luiza Helena, da Silveira, Rafael Bertoni, Sennf-Ribeiro, Andrea, Chaim, Olga Meiri, Veiga, Silvio Sanches, Arni, Raghuvir Krishnaswamy [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1107/S1744309110050931
http://hdl.handle.net/11449/22098
Resumo: Phospholipases D are the major dermonecrotic component of Loxosceles venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses. Phospholipases D can be classified into two classes depending on their catalytic efficiency and the presence of an additional disulfide bridge. In this work, both wild-type and H12A-mutant forms of the class II phospholipase D from L. intermedia venom were crystallized. Wild-type and H12A-mutant crystals were grown under very similar conditions using PEG 200 as a precipitant and belonged to space group P12(1)1, with unit-cell parameters a = 50.1, b = 49.5, c = 56.5 angstrom, beta = 105.9 degrees. Wild-type and H12A-mutant crystals diffracted to maximum resolutions of 1.95 and 1.60 angstrom, respectively.
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spelling Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venomPhospholipases D are the major dermonecrotic component of Loxosceles venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses. Phospholipases D can be classified into two classes depending on their catalytic efficiency and the presence of an additional disulfide bridge. In this work, both wild-type and H12A-mutant forms of the class II phospholipase D from L. intermedia venom were crystallized. Wild-type and H12A-mutant crystals were grown under very similar conditions using PEG 200 as a precipitant and belonged to space group P12(1)1, with unit-cell parameters a = 50.1, b = 49.5, c = 56.5 angstrom, beta = 105.9 degrees. Wild-type and H12A-mutant crystals diffracted to maximum resolutions of 1.95 and 1.60 angstrom, respectively.Secretaria de Estado de Ciencia, Tecnologia e Ensino Superior (SETI) do ParanaFundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná (FAADCT/PR)TWASFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, UNESP, Dept Fis, Ctr Multiusuario Inovacao Biomol, BR-15054000 Sao Jose do Rio Preto, SP, BrazilCtr Nacl Pesquisa Energia & Mat, LNBio, BR-13083970 Campinas, SP, BrazilUniv Fed Parana, Dept Biol Celular, BR-81531960 Curitiba, Parana, BrazilUniv Estadual Ponta Grossa, Dept Biol Estrutural Biol Mol & Genet, BR-84030900 Ponta Grossa, PR, BrazilUniv Estadual Paulista, UNESP, Dept Fis, Ctr Multiusuario Inovacao Biomol, BR-15054000 Sao Jose do Rio Preto, SP, BrazilWiley-BlackwellUniversidade Estadual Paulista (Unesp)Ctr Nacl Pesquisa Energia & MatUniversidade Federal do Paraná (UFPR)Univ Estadual Ponta GrossaUllah, Anwar [UNESP]de Giuseppe, Priscila OliveiraMurakami, Mario TyagoTrevisan-Silva, DilzaMartins Wille, Ana CarolinaChaves-Moreira, DanieleGremski, Luiza Helenada Silveira, Rafael BertoniSennf-Ribeiro, AndreaChaim, Olga MeiriVeiga, Silvio SanchesArni, Raghuvir Krishnaswamy [UNESP]2014-05-20T14:02:41Z2014-05-20T14:02:41Z2011-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article234-236application/pdfhttp://dx.doi.org/10.1107/S1744309110050931Acta Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell, v. 67, p. 234-236, 2011.1744-3091http://hdl.handle.net/11449/2209810.1107/S1744309110050931WOS:000287030600013WOS000287030600013.pdf91625089789458870000-0003-2460-1145Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Crystallographica Section F: Structural Biology and Crystallization Communicationsinfo:eu-repo/semantics/openAccess2023-12-22T06:23:54Zoai:repositorio.unesp.br:11449/22098Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:01:41.514540Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom
title Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom
spellingShingle Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom
Ullah, Anwar [UNESP]
title_short Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom
title_full Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom
title_fullStr Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom
title_full_unstemmed Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom
title_sort Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom
author Ullah, Anwar [UNESP]
author_facet Ullah, Anwar [UNESP]
de Giuseppe, Priscila Oliveira
Murakami, Mario Tyago
Trevisan-Silva, Dilza
Martins Wille, Ana Carolina
Chaves-Moreira, Daniele
Gremski, Luiza Helena
da Silveira, Rafael Bertoni
Sennf-Ribeiro, Andrea
Chaim, Olga Meiri
Veiga, Silvio Sanches
Arni, Raghuvir Krishnaswamy [UNESP]
author_role author
author2 de Giuseppe, Priscila Oliveira
Murakami, Mario Tyago
Trevisan-Silva, Dilza
Martins Wille, Ana Carolina
Chaves-Moreira, Daniele
Gremski, Luiza Helena
da Silveira, Rafael Bertoni
Sennf-Ribeiro, Andrea
Chaim, Olga Meiri
Veiga, Silvio Sanches
Arni, Raghuvir Krishnaswamy [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Ctr Nacl Pesquisa Energia & Mat
Universidade Federal do Paraná (UFPR)
Univ Estadual Ponta Grossa
dc.contributor.author.fl_str_mv Ullah, Anwar [UNESP]
de Giuseppe, Priscila Oliveira
Murakami, Mario Tyago
Trevisan-Silva, Dilza
Martins Wille, Ana Carolina
Chaves-Moreira, Daniele
Gremski, Luiza Helena
da Silveira, Rafael Bertoni
Sennf-Ribeiro, Andrea
Chaim, Olga Meiri
Veiga, Silvio Sanches
Arni, Raghuvir Krishnaswamy [UNESP]
description Phospholipases D are the major dermonecrotic component of Loxosceles venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses. Phospholipases D can be classified into two classes depending on their catalytic efficiency and the presence of an additional disulfide bridge. In this work, both wild-type and H12A-mutant forms of the class II phospholipase D from L. intermedia venom were crystallized. Wild-type and H12A-mutant crystals were grown under very similar conditions using PEG 200 as a precipitant and belonged to space group P12(1)1, with unit-cell parameters a = 50.1, b = 49.5, c = 56.5 angstrom, beta = 105.9 degrees. Wild-type and H12A-mutant crystals diffracted to maximum resolutions of 1.95 and 1.60 angstrom, respectively.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-01
2014-05-20T14:02:41Z
2014-05-20T14:02:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1107/S1744309110050931
Acta Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell, v. 67, p. 234-236, 2011.
1744-3091
http://hdl.handle.net/11449/22098
10.1107/S1744309110050931
WOS:000287030600013
WOS000287030600013.pdf
9162508978945887
0000-0003-2460-1145
url http://dx.doi.org/10.1107/S1744309110050931
http://hdl.handle.net/11449/22098
identifier_str_mv Acta Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell, v. 67, p. 234-236, 2011.
1744-3091
10.1107/S1744309110050931
WOS:000287030600013
WOS000287030600013.pdf
9162508978945887
0000-0003-2460-1145
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Crystallographica Section F: Structural Biology and Crystallization Communications
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 234-236
application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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