Crystallization and preliminary X-ray diffraction studies of BnSP-6, a myotoxic phospholipase A2 from Bothrops neuwiedi venom

Detalhes bibliográficos
Autor(a) principal: Fontes, Marcos R. M. [UNESP]
Data de Publicação: 2000
Outros Autores: Monteiro, Luiz E. [UNESP], Soares, Andreimar M., Rodrigues, Veridiana M., Da Silva, Reinaldo J. [UNESP], Giglio, José R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/224375
Resumo: BnSP-6 (myotoxin I) is a phospholipase A2 homologue isolated from Bothrops neuwiedi pauloensis venom. Crystals of BnSP-6 were obtained which diffracted X-rays to 2.5 Å resolution using a synchrotron radiation source at room temperature and belong to space group P3121. The unit cell dimensions are a=b=57.7, c=131.1 Å. The structure was solved by molecular replacement using the coordinates of bothropstoxin I from B. jararacussu venom. There are two molecules in the asymmetric unit.
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spelling Crystallization and preliminary X-ray diffraction studies of BnSP-6, a myotoxic phospholipase A2 from Bothrops neuwiedi venomBnSP-6 (myotoxin I) is a phospholipase A2 homologue isolated from Bothrops neuwiedi pauloensis venom. Crystals of BnSP-6 were obtained which diffracted X-rays to 2.5 Å resolution using a synchrotron radiation source at room temperature and belong to space group P3121. The unit cell dimensions are a=b=57.7, c=131.1 Å. The structure was solved by molecular replacement using the coordinates of bothropstoxin I from B. jararacussu venom. There are two molecules in the asymmetric unit.Depto. de Fis. e Biofísica Instituto de Biociências UNESP, C. P. 510, CEP 18618-000, Botucatu-SPDepartamento de Bioquímica Faculdade de Med. de Ribeirao Preto USP, Ribeirão Preto-SPCtro. Estudo Venenos Animais P. UNESP, Botucatu-SPDepto. de Fis. e Biofísica Instituto de Biociências UNESP, C. P. 510, CEP 18618-000, Botucatu-SPCtro. Estudo Venenos Animais P. UNESP, Botucatu-SPUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Fontes, Marcos R. M. [UNESP]Monteiro, Luiz E. [UNESP]Soares, Andreimar M.Rodrigues, Veridiana M.Da Silva, Reinaldo J. [UNESP]Giglio, José R.2022-04-28T19:56:09Z2022-04-28T19:56:09Z2000-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article421-425Protein and Peptide Letters, v. 7, n. 6, p. 421-425, 2000.0929-8665http://hdl.handle.net/11449/2243752-s2.0-0242434731Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengProtein and Peptide Lettersinfo:eu-repo/semantics/openAccess2022-04-28T19:56:09Zoai:repositorio.unesp.br:11449/224375Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:48:20.039819Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Crystallization and preliminary X-ray diffraction studies of BnSP-6, a myotoxic phospholipase A2 from Bothrops neuwiedi venom
title Crystallization and preliminary X-ray diffraction studies of BnSP-6, a myotoxic phospholipase A2 from Bothrops neuwiedi venom
spellingShingle Crystallization and preliminary X-ray diffraction studies of BnSP-6, a myotoxic phospholipase A2 from Bothrops neuwiedi venom
Fontes, Marcos R. M. [UNESP]
title_short Crystallization and preliminary X-ray diffraction studies of BnSP-6, a myotoxic phospholipase A2 from Bothrops neuwiedi venom
title_full Crystallization and preliminary X-ray diffraction studies of BnSP-6, a myotoxic phospholipase A2 from Bothrops neuwiedi venom
title_fullStr Crystallization and preliminary X-ray diffraction studies of BnSP-6, a myotoxic phospholipase A2 from Bothrops neuwiedi venom
title_full_unstemmed Crystallization and preliminary X-ray diffraction studies of BnSP-6, a myotoxic phospholipase A2 from Bothrops neuwiedi venom
title_sort Crystallization and preliminary X-ray diffraction studies of BnSP-6, a myotoxic phospholipase A2 from Bothrops neuwiedi venom
author Fontes, Marcos R. M. [UNESP]
author_facet Fontes, Marcos R. M. [UNESP]
Monteiro, Luiz E. [UNESP]
Soares, Andreimar M.
Rodrigues, Veridiana M.
Da Silva, Reinaldo J. [UNESP]
Giglio, José R.
author_role author
author2 Monteiro, Luiz E. [UNESP]
Soares, Andreimar M.
Rodrigues, Veridiana M.
Da Silva, Reinaldo J. [UNESP]
Giglio, José R.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Fontes, Marcos R. M. [UNESP]
Monteiro, Luiz E. [UNESP]
Soares, Andreimar M.
Rodrigues, Veridiana M.
Da Silva, Reinaldo J. [UNESP]
Giglio, José R.
description BnSP-6 (myotoxin I) is a phospholipase A2 homologue isolated from Bothrops neuwiedi pauloensis venom. Crystals of BnSP-6 were obtained which diffracted X-rays to 2.5 Å resolution using a synchrotron radiation source at room temperature and belong to space group P3121. The unit cell dimensions are a=b=57.7, c=131.1 Å. The structure was solved by molecular replacement using the coordinates of bothropstoxin I from B. jararacussu venom. There are two molecules in the asymmetric unit.
publishDate 2000
dc.date.none.fl_str_mv 2000-12-01
2022-04-28T19:56:09Z
2022-04-28T19:56:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Protein and Peptide Letters, v. 7, n. 6, p. 421-425, 2000.
0929-8665
http://hdl.handle.net/11449/224375
2-s2.0-0242434731
identifier_str_mv Protein and Peptide Letters, v. 7, n. 6, p. 421-425, 2000.
0929-8665
2-s2.0-0242434731
url http://hdl.handle.net/11449/224375
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Protein and Peptide Letters
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 421-425
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128277379809280

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