Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese mice
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-022-10688-w http://hdl.handle.net/11449/241645 |
Resumo: | Obesity is a disease characterized by the exacerbated increase of adipose tissue. A possible way to decrease the harmful effects of excessive adipose tissue is to increase the thermogenesis process, to the greater energy expenditure generated by the increase in heat in the body. In adipose tissue, the thermogenesis process is the result of an increase in mitochondrial work, having as substrate H+ ions, and which is related to the increased activity of UCP1. Evidence shows that stress is responsible for increasing the greater induction of UCP1 expression via β-adrenergic receptors. It is known that physical exercise is an important implement for sympathetic stimulation promoting communication between norepinephrine/epinephrine with membrane receptors. Thus, the present study investigates the influence of short-term strength training (STST) on fatty acid composition, lipolysis, lipogenesis, and browning processes in the subcutaneous adipose tissue (sWAT) of obese mice. For this, Swiss mice were divided into three groups: lean control, obesity sedentary, and obese strength training (OBexT). Obese animals were fed a high-fat diet for 14 weeks. Trained obese animals were submitted to 7 days of strength exercise. It was demonstrated that STST sessions were able to reduce fasting glycemia. In the sWAT, the STST was able to decrease the levels of the long-chain fatty acids profile, saturated fatty acid, and palmitic fatty acid (C16:0). Moreover, it was showed that STST did not increase protein levels responsible for lipolysis, the ATGL, ABHD5, pPLIN1, and pHSL. On the other hand, the exercise protocol decreased the expression of the lipogenic enzyme SCD1. Finally, our study demonstrated that the STST increased browning process-related genes such as PGC-1α, PRDM16, and UCP1 in the sWAT. Interestingly, all these biomolecular mechanisms have been observed independently of changes in body weight. Therefore, it is concluded that short-term strength exercise can be an effective strategy to initiate morphological changes in sWAT. |
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Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese miceObesity is a disease characterized by the exacerbated increase of adipose tissue. A possible way to decrease the harmful effects of excessive adipose tissue is to increase the thermogenesis process, to the greater energy expenditure generated by the increase in heat in the body. In adipose tissue, the thermogenesis process is the result of an increase in mitochondrial work, having as substrate H+ ions, and which is related to the increased activity of UCP1. Evidence shows that stress is responsible for increasing the greater induction of UCP1 expression via β-adrenergic receptors. It is known that physical exercise is an important implement for sympathetic stimulation promoting communication between norepinephrine/epinephrine with membrane receptors. Thus, the present study investigates the influence of short-term strength training (STST) on fatty acid composition, lipolysis, lipogenesis, and browning processes in the subcutaneous adipose tissue (sWAT) of obese mice. For this, Swiss mice were divided into three groups: lean control, obesity sedentary, and obese strength training (OBexT). Obese animals were fed a high-fat diet for 14 weeks. Trained obese animals were submitted to 7 days of strength exercise. It was demonstrated that STST sessions were able to reduce fasting glycemia. In the sWAT, the STST was able to decrease the levels of the long-chain fatty acids profile, saturated fatty acid, and palmitic fatty acid (C16:0). Moreover, it was showed that STST did not increase protein levels responsible for lipolysis, the ATGL, ABHD5, pPLIN1, and pHSL. On the other hand, the exercise protocol decreased the expression of the lipogenic enzyme SCD1. Finally, our study demonstrated that the STST increased browning process-related genes such as PGC-1α, PRDM16, and UCP1 in the sWAT. Interestingly, all these biomolecular mechanisms have been observed independently of changes in body weight. Therefore, it is concluded that short-term strength exercise can be an effective strategy to initiate morphological changes in sWAT.Exercise Cellular Biology Laboratory University of Campinas, São PauloLaboratory of Molecular Biology of Exercise School of Applied Sciences University of Campinas, São PauloDepartment of Physical Education Institute of Biosciences São Paulo State University (UNESP), São PauloLaboratory of Nutritional Genomics School of Applied Sciences University of Campinas, São PauloPostgraduate Program in Rehabilitation and Functional Performance Ribeirão Preto Medical School USP, São PauloSchool of Physical Education and Sport of Ribeirão Preto University of São Paulo (USP), São PauloDepartment of Physical Education Institute of Biosciences São Paulo State University (UNESP), São PauloUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)de Melo, Diego GomesAnaruma, Chadi Pellegrini [UNESP]da Cruz Rodrigues, Kellen CristinaPereira, Rodrigo Martinsde Campos, Thais Dantis PereiraCanciglieri, Raphael SantosRamos, Camila OliveiraCintra, Dennys EsperRopelle, Eduardo Rocheteda Silva, Adelino Sanchez RamosPauli, José Rodrigode Moura, Leandro Pereira2023-03-01T21:14:38Z2023-03-01T21:14:38Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-022-10688-wScientific Reports, v. 12, n. 1, 2022.2045-2322http://hdl.handle.net/11449/24164510.1038/s41598-022-10688-w2-s2.0-85128952748Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2024-08-14T17:23:20Zoai:repositorio.unesp.br:11449/241645Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese mice |
title |
Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese mice |
spellingShingle |
Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese mice de Melo, Diego Gomes |
title_short |
Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese mice |
title_full |
Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese mice |
title_fullStr |
Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese mice |
title_full_unstemmed |
Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese mice |
title_sort |
Strength training alters the tissue fatty acids profile and slightly improves the thermogenic pathway in the adipose tissue of obese mice |
author |
de Melo, Diego Gomes |
author_facet |
de Melo, Diego Gomes Anaruma, Chadi Pellegrini [UNESP] da Cruz Rodrigues, Kellen Cristina Pereira, Rodrigo Martins de Campos, Thais Dantis Pereira Canciglieri, Raphael Santos Ramos, Camila Oliveira Cintra, Dennys Esper Ropelle, Eduardo Rochete da Silva, Adelino Sanchez Ramos Pauli, José Rodrigo de Moura, Leandro Pereira |
author_role |
author |
author2 |
Anaruma, Chadi Pellegrini [UNESP] da Cruz Rodrigues, Kellen Cristina Pereira, Rodrigo Martins de Campos, Thais Dantis Pereira Canciglieri, Raphael Santos Ramos, Camila Oliveira Cintra, Dennys Esper Ropelle, Eduardo Rochete da Silva, Adelino Sanchez Ramos Pauli, José Rodrigo de Moura, Leandro Pereira |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
de Melo, Diego Gomes Anaruma, Chadi Pellegrini [UNESP] da Cruz Rodrigues, Kellen Cristina Pereira, Rodrigo Martins de Campos, Thais Dantis Pereira Canciglieri, Raphael Santos Ramos, Camila Oliveira Cintra, Dennys Esper Ropelle, Eduardo Rochete da Silva, Adelino Sanchez Ramos Pauli, José Rodrigo de Moura, Leandro Pereira |
description |
Obesity is a disease characterized by the exacerbated increase of adipose tissue. A possible way to decrease the harmful effects of excessive adipose tissue is to increase the thermogenesis process, to the greater energy expenditure generated by the increase in heat in the body. In adipose tissue, the thermogenesis process is the result of an increase in mitochondrial work, having as substrate H+ ions, and which is related to the increased activity of UCP1. Evidence shows that stress is responsible for increasing the greater induction of UCP1 expression via β-adrenergic receptors. It is known that physical exercise is an important implement for sympathetic stimulation promoting communication between norepinephrine/epinephrine with membrane receptors. Thus, the present study investigates the influence of short-term strength training (STST) on fatty acid composition, lipolysis, lipogenesis, and browning processes in the subcutaneous adipose tissue (sWAT) of obese mice. For this, Swiss mice were divided into three groups: lean control, obesity sedentary, and obese strength training (OBexT). Obese animals were fed a high-fat diet for 14 weeks. Trained obese animals were submitted to 7 days of strength exercise. It was demonstrated that STST sessions were able to reduce fasting glycemia. In the sWAT, the STST was able to decrease the levels of the long-chain fatty acids profile, saturated fatty acid, and palmitic fatty acid (C16:0). Moreover, it was showed that STST did not increase protein levels responsible for lipolysis, the ATGL, ABHD5, pPLIN1, and pHSL. On the other hand, the exercise protocol decreased the expression of the lipogenic enzyme SCD1. Finally, our study demonstrated that the STST increased browning process-related genes such as PGC-1α, PRDM16, and UCP1 in the sWAT. Interestingly, all these biomolecular mechanisms have been observed independently of changes in body weight. Therefore, it is concluded that short-term strength exercise can be an effective strategy to initiate morphological changes in sWAT. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-03-01T21:14:38Z 2023-03-01T21:14:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-022-10688-w Scientific Reports, v. 12, n. 1, 2022. 2045-2322 http://hdl.handle.net/11449/241645 10.1038/s41598-022-10688-w 2-s2.0-85128952748 |
url |
http://dx.doi.org/10.1038/s41598-022-10688-w http://hdl.handle.net/11449/241645 |
identifier_str_mv |
Scientific Reports, v. 12, n. 1, 2022. 2045-2322 10.1038/s41598-022-10688-w 2-s2.0-85128952748 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128156321710080 |