Effect of Byrsonima crassa and phenolic constituents on Helicobacter pylori-induced neutrophils oxidative burst
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms13010133 http://hdl.handle.net/11449/232052 |
Resumo: | Byrsonima crassa Niedenzu (Malpighiaceae) is used in Brazilian folk medicine for the treatment of diseases related mainly to gastric ulcers. In a previous study, our group described the gastric protective effect of the methanolic extract from the leaves of B. crassa. The present study was carried out to investigate the effects of methanolic extract and its phenolic compounds on the respiratory burst of neutrophils stimulated by H. pylori using a luminol-based chemiluminescence assay as well as their anti-H. pylori activity. The suppressive activity on oxidative burst of H. pylori-stimulated neutrophils was in the order of methyl gallate > (+)-catechin > methanol extract > quercetin 3-O-α-Larabinopyranoside > quercetin 3-O-β-D-galactopyranoside > amentoflavone. Methyl gallate, compound that induced the highest suppressive activity with IC50 value of 3.4 μg/mL, did not show anti-H. pylori activity. B. crassa could be considered as a potential source of natural antioxidant in gastric ulcers by attenuating the effects on the damage to gastric mucosa caused by neutrophil generated reactive oxygen species, even when H. pylori displays its evasion mechanisms. © 2012 by the authors; licensee MDPI, Basel, Switzerland. |
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Effect of Byrsonima crassa and phenolic constituents on Helicobacter pylori-induced neutrophils oxidative burstAntioxidant activityByrsonima crassaChemiluminescenceGastric ulcersHelicobacter pyloriPhenolic compoundsByrsonima crassa Niedenzu (Malpighiaceae) is used in Brazilian folk medicine for the treatment of diseases related mainly to gastric ulcers. In a previous study, our group described the gastric protective effect of the methanolic extract from the leaves of B. crassa. The present study was carried out to investigate the effects of methanolic extract and its phenolic compounds on the respiratory burst of neutrophils stimulated by H. pylori using a luminol-based chemiluminescence assay as well as their anti-H. pylori activity. The suppressive activity on oxidative burst of H. pylori-stimulated neutrophils was in the order of methyl gallate > (+)-catechin > methanol extract > quercetin 3-O-α-Larabinopyranoside > quercetin 3-O-β-D-galactopyranoside > amentoflavone. Methyl gallate, compound that induced the highest suppressive activity with IC50 value of 3.4 μg/mL, did not show anti-H. pylori activity. B. crassa could be considered as a potential source of natural antioxidant in gastric ulcers by attenuating the effects on the damage to gastric mucosa caused by neutrophil generated reactive oxygen species, even when H. pylori displays its evasion mechanisms. © 2012 by the authors; licensee MDPI, Basel, Switzerland.School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, SP, 14801-902School of Arts, Sciences and Humanities, University of São Paulo (USP), São Paulo, SP, 03828-000Institute of Chemistry, São Paulo State University (UNESP), Araraquara, SP, 14800-900School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara, SP, 14801-902Institute of Chemistry, São Paulo State University (UNESP), Araraquara, SP, 14800-900Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Bonacorsi, Cibele [UNESP]Raddi, Maria Stella G. [UNESP]da Fonseca, Luiz Marcos [UNESP]Sannomiya, MiriamVilegas, Wagner [UNESP]2022-04-29T08:48:49Z2022-04-29T08:48:49Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article133-141http://dx.doi.org/10.3390/ijms13010133International Journal of Molecular Sciences, v. 13, n. 1, p. 133-141, 2012.1422-0067http://hdl.handle.net/11449/23205210.3390/ijms130101332-s2.0-84856256630Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2022-04-29T08:48:49Zoai:repositorio.unesp.br:11449/232052Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:05:55.015233Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of Byrsonima crassa and phenolic constituents on Helicobacter pylori-induced neutrophils oxidative burst |
title |
Effect of Byrsonima crassa and phenolic constituents on Helicobacter pylori-induced neutrophils oxidative burst |
spellingShingle |
Effect of Byrsonima crassa and phenolic constituents on Helicobacter pylori-induced neutrophils oxidative burst Bonacorsi, Cibele [UNESP] Antioxidant activity Byrsonima crassa Chemiluminescence Gastric ulcers Helicobacter pylori Phenolic compounds |
title_short |
Effect of Byrsonima crassa and phenolic constituents on Helicobacter pylori-induced neutrophils oxidative burst |
title_full |
Effect of Byrsonima crassa and phenolic constituents on Helicobacter pylori-induced neutrophils oxidative burst |
title_fullStr |
Effect of Byrsonima crassa and phenolic constituents on Helicobacter pylori-induced neutrophils oxidative burst |
title_full_unstemmed |
Effect of Byrsonima crassa and phenolic constituents on Helicobacter pylori-induced neutrophils oxidative burst |
title_sort |
Effect of Byrsonima crassa and phenolic constituents on Helicobacter pylori-induced neutrophils oxidative burst |
author |
Bonacorsi, Cibele [UNESP] |
author_facet |
Bonacorsi, Cibele [UNESP] Raddi, Maria Stella G. [UNESP] da Fonseca, Luiz Marcos [UNESP] Sannomiya, Miriam Vilegas, Wagner [UNESP] |
author_role |
author |
author2 |
Raddi, Maria Stella G. [UNESP] da Fonseca, Luiz Marcos [UNESP] Sannomiya, Miriam Vilegas, Wagner [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Bonacorsi, Cibele [UNESP] Raddi, Maria Stella G. [UNESP] da Fonseca, Luiz Marcos [UNESP] Sannomiya, Miriam Vilegas, Wagner [UNESP] |
dc.subject.por.fl_str_mv |
Antioxidant activity Byrsonima crassa Chemiluminescence Gastric ulcers Helicobacter pylori Phenolic compounds |
topic |
Antioxidant activity Byrsonima crassa Chemiluminescence Gastric ulcers Helicobacter pylori Phenolic compounds |
description |
Byrsonima crassa Niedenzu (Malpighiaceae) is used in Brazilian folk medicine for the treatment of diseases related mainly to gastric ulcers. In a previous study, our group described the gastric protective effect of the methanolic extract from the leaves of B. crassa. The present study was carried out to investigate the effects of methanolic extract and its phenolic compounds on the respiratory burst of neutrophils stimulated by H. pylori using a luminol-based chemiluminescence assay as well as their anti-H. pylori activity. The suppressive activity on oxidative burst of H. pylori-stimulated neutrophils was in the order of methyl gallate > (+)-catechin > methanol extract > quercetin 3-O-α-Larabinopyranoside > quercetin 3-O-β-D-galactopyranoside > amentoflavone. Methyl gallate, compound that induced the highest suppressive activity with IC50 value of 3.4 μg/mL, did not show anti-H. pylori activity. B. crassa could be considered as a potential source of natural antioxidant in gastric ulcers by attenuating the effects on the damage to gastric mucosa caused by neutrophil generated reactive oxygen species, even when H. pylori displays its evasion mechanisms. © 2012 by the authors; licensee MDPI, Basel, Switzerland. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2022-04-29T08:48:49Z 2022-04-29T08:48:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms13010133 International Journal of Molecular Sciences, v. 13, n. 1, p. 133-141, 2012. 1422-0067 http://hdl.handle.net/11449/232052 10.3390/ijms13010133 2-s2.0-84856256630 |
url |
http://dx.doi.org/10.3390/ijms13010133 http://hdl.handle.net/11449/232052 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 13, n. 1, p. 133-141, 2012. 1422-0067 10.3390/ijms13010133 2-s2.0-84856256630 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
133-141 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129582501462016 |