Chemical characterization, hydrolysis and bioethanol production from municipal solid waste

Detalhes bibliográficos
Autor(a) principal: Oliveira, Fabíola Ribeiro de
Data de Publicação: 2022
Tipo de documento: Trabalho de conclusão de curso
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/216276
Resumo: Food waste is increasing in the world and this residue when inappropriately disposed, cause serious problems in the environment, due to its toxicity for soil and water. Then, new technologies for the use of municipal solid waste (MSW) can decrease its damage to nature and expenses for society, besides open up the opportunity to obtain value-added products.There is also a strong interest in the development of fuels from renewable sources, aiming at lower environmental impacts than the widely used fossil fuels. In view of this, biomass from MSW is known for its great potential as a source for biofuels and biomolecules production. Therefore, the present work aimed to produce ethanol from the organic portion of the MSW. The proposed technology conforms to the concept of biorefinery, and it consisted in the enzymatic hydrolysis using glucoamylases produced by Rhizopus oligosporus in solid-state cultivation, through technology patented by UNESP (BR 102014031591-8 A2), which showed a conversion capacity of starch to reducing sugars (RS) of 67.38%, followed by alcoholic fermentation by Saccharomyces cerevisiae, producing 0.25 g of ethanol per gram of reducing sugar offered. Therefore, it was found that use of this lower cost source of enzyme results in good hydrolysis yielding, and consequently, considerable bioethanol production from MSW.
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spelling Chemical characterization, hydrolysis and bioethanol production from municipal solid wasteCaracterização química, hidrólise e produção de bioetanol a partir de resíduos sólidos urbanosBiomassaBiorrefinariaFermentaçãoAçúcares redutoresHidrólise enzimáticaFood waste is increasing in the world and this residue when inappropriately disposed, cause serious problems in the environment, due to its toxicity for soil and water. Then, new technologies for the use of municipal solid waste (MSW) can decrease its damage to nature and expenses for society, besides open up the opportunity to obtain value-added products.There is also a strong interest in the development of fuels from renewable sources, aiming at lower environmental impacts than the widely used fossil fuels. In view of this, biomass from MSW is known for its great potential as a source for biofuels and biomolecules production. Therefore, the present work aimed to produce ethanol from the organic portion of the MSW. The proposed technology conforms to the concept of biorefinery, and it consisted in the enzymatic hydrolysis using glucoamylases produced by Rhizopus oligosporus in solid-state cultivation, through technology patented by UNESP (BR 102014031591-8 A2), which showed a conversion capacity of starch to reducing sugars (RS) of 67.38%, followed by alcoholic fermentation by Saccharomyces cerevisiae, producing 0.25 g of ethanol per gram of reducing sugar offered. Therefore, it was found that use of this lower cost source of enzyme results in good hydrolysis yielding, and consequently, considerable bioethanol production from MSW.O desperdício de alimentos está aumentando no mundo e esse resíduo quando descartado de forma inadequada, causa sérios problemas ao meio ambiente, devido a sua toxicidade para o solo e a água. Assim, novas tecnologias de aproveitamento de resíduos sólidos urbanos (RSU) podem diminuir seus danos à natureza e gastos para a sociedade, além de abrir a oportunidade de obtenção de produtos de valor agregado. Existe ainda, um grande interesse no desenvolvimento de combustíveis provindos de fontes renováveis, visando impactos ambientais menores do que os combustíveis fósseis amplamente utilizados. Diante disso, a biomassa de RSU é conhecida por seu grande potencial como fonte para produção de biocombustíveis e biomoléculas. Portanto, o presente trabalho teve como objetivo produzir etanol a partir da porção orgânica do RSU. A tecnologia proposta está em conformidade com o conceito de biorrefinaria, e consistiu na hidrólise enzimática utilizando glucoamilases produzidas por Rhizopus oligosporus em cultivo em estado sólido, por meio de tecnologia patenteada pela UNESP (BR 102014031591-8 A2), que mostrou capacidade de conversão de amido em açúcares redutores (AR) de 67,38%, seguido de fermentação alcoólica por Saccharomyces cerevisiae, produzindo 0,25 g de etanol por grama de açúcar redutor ofertado. Portanto, verificou-se que o uso desta fonte de enzima de menor custo resulta em um bom rendimento de hidrólise e, consequentemente, uma produção considerável de bioetanol a partir de RSU.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 2019/24970-5Universidade Estadual Paulista (Unesp)Oliva Neto, Pedro de [UNESP]Escaramboni, BrunaUniversidade Estadual Paulista (Unesp)Oliveira, Fabíola Ribeiro de2022-02-02T17:29:52Z2022-02-02T17:29:52Z2022-01-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttp://hdl.handle.net/11449/216276enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2023-12-17T06:21:38Zoai:repositorio.unesp.br:11449/216276Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:34:39.321162Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Chemical characterization, hydrolysis and bioethanol production from municipal solid waste
Caracterização química, hidrólise e produção de bioetanol a partir de resíduos sólidos urbanos
title Chemical characterization, hydrolysis and bioethanol production from municipal solid waste
spellingShingle Chemical characterization, hydrolysis and bioethanol production from municipal solid waste
Oliveira, Fabíola Ribeiro de
Biomassa
Biorrefinaria
Fermentação
Açúcares redutores
Hidrólise enzimática
title_short Chemical characterization, hydrolysis and bioethanol production from municipal solid waste
title_full Chemical characterization, hydrolysis and bioethanol production from municipal solid waste
title_fullStr Chemical characterization, hydrolysis and bioethanol production from municipal solid waste
title_full_unstemmed Chemical characterization, hydrolysis and bioethanol production from municipal solid waste
title_sort Chemical characterization, hydrolysis and bioethanol production from municipal solid waste
author Oliveira, Fabíola Ribeiro de
author_facet Oliveira, Fabíola Ribeiro de
author_role author
dc.contributor.none.fl_str_mv Oliva Neto, Pedro de [UNESP]
Escaramboni, Bruna
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Oliveira, Fabíola Ribeiro de
dc.subject.por.fl_str_mv Biomassa
Biorrefinaria
Fermentação
Açúcares redutores
Hidrólise enzimática
topic Biomassa
Biorrefinaria
Fermentação
Açúcares redutores
Hidrólise enzimática
description Food waste is increasing in the world and this residue when inappropriately disposed, cause serious problems in the environment, due to its toxicity for soil and water. Then, new technologies for the use of municipal solid waste (MSW) can decrease its damage to nature and expenses for society, besides open up the opportunity to obtain value-added products.There is also a strong interest in the development of fuels from renewable sources, aiming at lower environmental impacts than the widely used fossil fuels. In view of this, biomass from MSW is known for its great potential as a source for biofuels and biomolecules production. Therefore, the present work aimed to produce ethanol from the organic portion of the MSW. The proposed technology conforms to the concept of biorefinery, and it consisted in the enzymatic hydrolysis using glucoamylases produced by Rhizopus oligosporus in solid-state cultivation, through technology patented by UNESP (BR 102014031591-8 A2), which showed a conversion capacity of starch to reducing sugars (RS) of 67.38%, followed by alcoholic fermentation by Saccharomyces cerevisiae, producing 0.25 g of ethanol per gram of reducing sugar offered. Therefore, it was found that use of this lower cost source of enzyme results in good hydrolysis yielding, and consequently, considerable bioethanol production from MSW.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-02T17:29:52Z
2022-02-02T17:29:52Z
2022-01-20
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/11449/216276
url http://hdl.handle.net/11449/216276
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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