Chemical characterization, hydrolysis and bioethanol production from municipal solid waste
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/216276 |
Resumo: | Food waste is increasing in the world and this residue when inappropriately disposed, cause serious problems in the environment, due to its toxicity for soil and water. Then, new technologies for the use of municipal solid waste (MSW) can decrease its damage to nature and expenses for society, besides open up the opportunity to obtain value-added products.There is also a strong interest in the development of fuels from renewable sources, aiming at lower environmental impacts than the widely used fossil fuels. In view of this, biomass from MSW is known for its great potential as a source for biofuels and biomolecules production. Therefore, the present work aimed to produce ethanol from the organic portion of the MSW. The proposed technology conforms to the concept of biorefinery, and it consisted in the enzymatic hydrolysis using glucoamylases produced by Rhizopus oligosporus in solid-state cultivation, through technology patented by UNESP (BR 102014031591-8 A2), which showed a conversion capacity of starch to reducing sugars (RS) of 67.38%, followed by alcoholic fermentation by Saccharomyces cerevisiae, producing 0.25 g of ethanol per gram of reducing sugar offered. Therefore, it was found that use of this lower cost source of enzyme results in good hydrolysis yielding, and consequently, considerable bioethanol production from MSW. |
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Chemical characterization, hydrolysis and bioethanol production from municipal solid wasteCaracterização química, hidrólise e produção de bioetanol a partir de resíduos sólidos urbanosBiomassaBiorrefinariaFermentaçãoAçúcares redutoresHidrólise enzimáticaFood waste is increasing in the world and this residue when inappropriately disposed, cause serious problems in the environment, due to its toxicity for soil and water. Then, new technologies for the use of municipal solid waste (MSW) can decrease its damage to nature and expenses for society, besides open up the opportunity to obtain value-added products.There is also a strong interest in the development of fuels from renewable sources, aiming at lower environmental impacts than the widely used fossil fuels. In view of this, biomass from MSW is known for its great potential as a source for biofuels and biomolecules production. Therefore, the present work aimed to produce ethanol from the organic portion of the MSW. The proposed technology conforms to the concept of biorefinery, and it consisted in the enzymatic hydrolysis using glucoamylases produced by Rhizopus oligosporus in solid-state cultivation, through technology patented by UNESP (BR 102014031591-8 A2), which showed a conversion capacity of starch to reducing sugars (RS) of 67.38%, followed by alcoholic fermentation by Saccharomyces cerevisiae, producing 0.25 g of ethanol per gram of reducing sugar offered. Therefore, it was found that use of this lower cost source of enzyme results in good hydrolysis yielding, and consequently, considerable bioethanol production from MSW.O desperdício de alimentos está aumentando no mundo e esse resíduo quando descartado de forma inadequada, causa sérios problemas ao meio ambiente, devido a sua toxicidade para o solo e a água. Assim, novas tecnologias de aproveitamento de resíduos sólidos urbanos (RSU) podem diminuir seus danos à natureza e gastos para a sociedade, além de abrir a oportunidade de obtenção de produtos de valor agregado. Existe ainda, um grande interesse no desenvolvimento de combustíveis provindos de fontes renováveis, visando impactos ambientais menores do que os combustíveis fósseis amplamente utilizados. Diante disso, a biomassa de RSU é conhecida por seu grande potencial como fonte para produção de biocombustíveis e biomoléculas. Portanto, o presente trabalho teve como objetivo produzir etanol a partir da porção orgânica do RSU. A tecnologia proposta está em conformidade com o conceito de biorrefinaria, e consistiu na hidrólise enzimática utilizando glucoamilases produzidas por Rhizopus oligosporus em cultivo em estado sólido, por meio de tecnologia patenteada pela UNESP (BR 102014031591-8 A2), que mostrou capacidade de conversão de amido em açúcares redutores (AR) de 67,38%, seguido de fermentação alcoólica por Saccharomyces cerevisiae, produzindo 0,25 g de etanol por grama de açúcar redutor ofertado. Portanto, verificou-se que o uso desta fonte de enzima de menor custo resulta em um bom rendimento de hidrólise e, consequentemente, uma produção considerável de bioetanol a partir de RSU.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 2019/24970-5Universidade Estadual Paulista (Unesp)Oliva Neto, Pedro de [UNESP]Escaramboni, BrunaUniversidade Estadual Paulista (Unesp)Oliveira, Fabíola Ribeiro de2022-02-02T17:29:52Z2022-02-02T17:29:52Z2022-01-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisapplication/pdfhttp://hdl.handle.net/11449/216276enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2023-12-17T06:21:38Zoai:repositorio.unesp.br:11449/216276Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:34:39.321162Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chemical characterization, hydrolysis and bioethanol production from municipal solid waste Caracterização química, hidrólise e produção de bioetanol a partir de resíduos sólidos urbanos |
title |
Chemical characterization, hydrolysis and bioethanol production from municipal solid waste |
spellingShingle |
Chemical characterization, hydrolysis and bioethanol production from municipal solid waste Oliveira, Fabíola Ribeiro de Biomassa Biorrefinaria Fermentação Açúcares redutores Hidrólise enzimática |
title_short |
Chemical characterization, hydrolysis and bioethanol production from municipal solid waste |
title_full |
Chemical characterization, hydrolysis and bioethanol production from municipal solid waste |
title_fullStr |
Chemical characterization, hydrolysis and bioethanol production from municipal solid waste |
title_full_unstemmed |
Chemical characterization, hydrolysis and bioethanol production from municipal solid waste |
title_sort |
Chemical characterization, hydrolysis and bioethanol production from municipal solid waste |
author |
Oliveira, Fabíola Ribeiro de |
author_facet |
Oliveira, Fabíola Ribeiro de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Oliva Neto, Pedro de [UNESP] Escaramboni, Bruna Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Oliveira, Fabíola Ribeiro de |
dc.subject.por.fl_str_mv |
Biomassa Biorrefinaria Fermentação Açúcares redutores Hidrólise enzimática |
topic |
Biomassa Biorrefinaria Fermentação Açúcares redutores Hidrólise enzimática |
description |
Food waste is increasing in the world and this residue when inappropriately disposed, cause serious problems in the environment, due to its toxicity for soil and water. Then, new technologies for the use of municipal solid waste (MSW) can decrease its damage to nature and expenses for society, besides open up the opportunity to obtain value-added products.There is also a strong interest in the development of fuels from renewable sources, aiming at lower environmental impacts than the widely used fossil fuels. In view of this, biomass from MSW is known for its great potential as a source for biofuels and biomolecules production. Therefore, the present work aimed to produce ethanol from the organic portion of the MSW. The proposed technology conforms to the concept of biorefinery, and it consisted in the enzymatic hydrolysis using glucoamylases produced by Rhizopus oligosporus in solid-state cultivation, through technology patented by UNESP (BR 102014031591-8 A2), which showed a conversion capacity of starch to reducing sugars (RS) of 67.38%, followed by alcoholic fermentation by Saccharomyces cerevisiae, producing 0.25 g of ethanol per gram of reducing sugar offered. Therefore, it was found that use of this lower cost source of enzyme results in good hydrolysis yielding, and consequently, considerable bioethanol production from MSW. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02-02T17:29:52Z 2022-02-02T17:29:52Z 2022-01-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
format |
bachelorThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/11449/216276 |
url |
http://hdl.handle.net/11449/216276 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129222959431680 |