Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572005000300022 http://hdl.handle.net/11449/68309 |
Resumo: | Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 → 6)β-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated phases); b) a mixture taken from AB 96/07, AB 96/09 and AB 97/ 11 strains; and c) commercial mushrooms from Londrina, PR and Piedade, SP, designated as AB PR and AB SP, respectively. The extracts from these mushrooms were isolated in chloroform:methanol (3:1) and used in vitro at three different concentrations. V79 cells (Chinese hamster lung cells) were exposed to the extracts under pre-, simultaneous and post-treatment conditions, combined with methyl methanesulfonate (MMS). Under the circumstances of this study, these organic extracts did not show any genotoxic or mutagenic effects, but did protect cells against the induction of micronuclei by MMS. Copyright by the Brazilian Society of Genetics. |
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Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cellsAgaricus blazeiComet assayMicronucleiMushroom extractsV79 cellsAgaricus blazei extractalcoholchloroformmesylic acid methyl esteranimal cellcell protectioncell strain V 79comet assaycontrolled studydrug activitydrug effectdrug exposuredrug isolationfungal straingenotoxicityin vitro studymicronucleusmushroommutagenicitynonhumanAnimaliaBasidiomycotaCricetulus griseusAgaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 → 6)β-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated phases); b) a mixture taken from AB 96/07, AB 96/09 and AB 97/ 11 strains; and c) commercial mushrooms from Londrina, PR and Piedade, SP, designated as AB PR and AB SP, respectively. The extracts from these mushrooms were isolated in chloroform:methanol (3:1) and used in vitro at three different concentrations. V79 cells (Chinese hamster lung cells) were exposed to the extracts under pre-, simultaneous and post-treatment conditions, combined with methyl methanesulfonate (MMS). Under the circumstances of this study, these organic extracts did not show any genotoxic or mutagenic effects, but did protect cells against the induction of micronuclei by MMS. Copyright by the Brazilian Society of Genetics.Universidade Estadual de Londrina Departamento de Biologia Geral, Caixa Postal 6001, 86.051-990 Londrina, PRUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agronômicas Departamento de Produção Vegetal, Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Departamento de Patologia, Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agronômicas Departamento de Produção Vegetal, Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Departamento de Patologia, Botucatu, SPUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)da Rosa Guterres, ZairaMantovani, Mário Sérgioda Eira, Augusto Ferreira [UNESP]Ribeiro, Lúcia Regina [UNESP]Jordão, Berenice Quinzani2014-05-27T11:21:22Z2014-05-27T11:21:22Z2005-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article458-463application/pdfhttp://dx.doi.org/10.1590/S1415-47572005000300022Genetics and Molecular Biology, v. 28, n. 3, p. 458-463, 2005.1415-47571678-4685http://hdl.handle.net/11449/6830910.1590/S1415-47572005000300022S1415-47572005000300022WOS:0002356532000212-s2.0-305444543032-s2.0-30544454303.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2024-04-30T15:58:33Zoai:repositorio.unesp.br:11449/68309Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-30T15:58:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells |
title |
Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells |
spellingShingle |
Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells da Rosa Guterres, Zaira Agaricus blazei Comet assay Micronuclei Mushroom extracts V79 cells Agaricus blazei extract alcohol chloroform mesylic acid methyl ester animal cell cell protection cell strain V 79 comet assay controlled study drug activity drug effect drug exposure drug isolation fungal strain genotoxicity in vitro study micronucleus mushroom mutagenicity nonhuman Animalia Basidiomycota Cricetulus griseus |
title_short |
Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells |
title_full |
Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells |
title_fullStr |
Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells |
title_full_unstemmed |
Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells |
title_sort |
Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells |
author |
da Rosa Guterres, Zaira |
author_facet |
da Rosa Guterres, Zaira Mantovani, Mário Sérgio da Eira, Augusto Ferreira [UNESP] Ribeiro, Lúcia Regina [UNESP] Jordão, Berenice Quinzani |
author_role |
author |
author2 |
Mantovani, Mário Sérgio da Eira, Augusto Ferreira [UNESP] Ribeiro, Lúcia Regina [UNESP] Jordão, Berenice Quinzani |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
da Rosa Guterres, Zaira Mantovani, Mário Sérgio da Eira, Augusto Ferreira [UNESP] Ribeiro, Lúcia Regina [UNESP] Jordão, Berenice Quinzani |
dc.subject.por.fl_str_mv |
Agaricus blazei Comet assay Micronuclei Mushroom extracts V79 cells Agaricus blazei extract alcohol chloroform mesylic acid methyl ester animal cell cell protection cell strain V 79 comet assay controlled study drug activity drug effect drug exposure drug isolation fungal strain genotoxicity in vitro study micronucleus mushroom mutagenicity nonhuman Animalia Basidiomycota Cricetulus griseus |
topic |
Agaricus blazei Comet assay Micronuclei Mushroom extracts V79 cells Agaricus blazei extract alcohol chloroform mesylic acid methyl ester animal cell cell protection cell strain V 79 comet assay controlled study drug activity drug effect drug exposure drug isolation fungal strain genotoxicity in vitro study micronucleus mushroom mutagenicity nonhuman Animalia Basidiomycota Cricetulus griseus |
description |
Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 → 6)β-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated phases); b) a mixture taken from AB 96/07, AB 96/09 and AB 97/ 11 strains; and c) commercial mushrooms from Londrina, PR and Piedade, SP, designated as AB PR and AB SP, respectively. The extracts from these mushrooms were isolated in chloroform:methanol (3:1) and used in vitro at three different concentrations. V79 cells (Chinese hamster lung cells) were exposed to the extracts under pre-, simultaneous and post-treatment conditions, combined with methyl methanesulfonate (MMS). Under the circumstances of this study, these organic extracts did not show any genotoxic or mutagenic effects, but did protect cells against the induction of micronuclei by MMS. Copyright by the Brazilian Society of Genetics. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-07-01 2014-05-27T11:21:22Z 2014-05-27T11:21:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572005000300022 Genetics and Molecular Biology, v. 28, n. 3, p. 458-463, 2005. 1415-4757 1678-4685 http://hdl.handle.net/11449/68309 10.1590/S1415-47572005000300022 S1415-47572005000300022 WOS:000235653200021 2-s2.0-30544454303 2-s2.0-30544454303.pdf |
url |
http://dx.doi.org/10.1590/S1415-47572005000300022 http://hdl.handle.net/11449/68309 |
identifier_str_mv |
Genetics and Molecular Biology, v. 28, n. 3, p. 458-463, 2005. 1415-4757 1678-4685 10.1590/S1415-47572005000300022 S1415-47572005000300022 WOS:000235653200021 2-s2.0-30544454303 2-s2.0-30544454303.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
458-463 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965497008062464 |