Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells

Detalhes bibliográficos
Autor(a) principal: Saraiva, Patricia R. [UNESP]
Data de Publicação: 2008
Outros Autores: Teixeira, Silvania S. [UNESP], Nogueira, Célia Regina [UNESP], Padovani, Carlos Roberto [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0004-27302008000100015
http://hdl.handle.net/11449/11377
Resumo: Osteoclastogenesis may be regulated via activation of the RANK/RANKL (receptor activator of nuclear factor-kappa B/ receptor activator of nuclear factor-kappa B ligand) system, which is mediated by osteoblasts. However, the bone loss mechanism induced by T3 (triiodothyronine) is still controversial. In this study, osteoblastic lineage rat cells (ROS 17/2.8) were treated with T3 (10(-8) M 10(-9) 10 M, and 10(-10) M), and RANKL mRNA (messenger RNA) expression was measured by semiquantitative RT-PCR. Our results show that T3 concentrations used did not significantly enhance RANKL expression compared to controls without hormone treatment. This data suggests that other mechanisms, unrelated to the RANK/RANKL system, might be to activate osteoclast differentiation in these cells.
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spelling Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cellsTriiodotironina (T3) não induz a expressão de Rankl em células de rato ROS 17/2.8thyroid hormoneRANKLboneratROS17/2.8 cellOsteoclastogenesis may be regulated via activation of the RANK/RANKL (receptor activator of nuclear factor-kappa B/ receptor activator of nuclear factor-kappa B ligand) system, which is mediated by osteoblasts. However, the bone loss mechanism induced by T3 (triiodothyronine) is still controversial. In this study, osteoblastic lineage rat cells (ROS 17/2.8) were treated with T3 (10(-8) M 10(-9) 10 M, and 10(-10) M), and RANKL mRNA (messenger RNA) expression was measured by semiquantitative RT-PCR. Our results show that T3 concentrations used did not significantly enhance RANKL expression compared to controls without hormone treatment. This data suggests that other mechanisms, unrelated to the RANK/RANKL system, might be to activate osteoclast differentiation in these cells.A osteoclastogênese pode ser regulada via ativação do sistema RANK/RANKL (receptor ativador do fator nuclear kapa B/ ligante do receptor do fator nuclear kapa B), que é mediado pelos osteoblastos. Entretanto, o mecanismo de perda óssea induzido pelo T3 (triiodotironina) ainda é controverso. Neste estudo, a linhagem osteoblástica de células de rato ROS 17/2.8 foi tratada com T3 (10-8 M, 10-9 M e 10-10 M), e a expressão do mRNA do RANKL foi medida por RT-PCR semiquantitativo. Nossos resultados mostraram que as concentrações de T3 utilizadas não induziram significativamente a expressão do RANKL, comparado ao controle (sem tratamento hormonal). Estes dados sugerem que outros mecanismos, não relacionados ao sistema RANK/RANKL, são usados para ativar a diferenciação osteoclástica nestas células.São Paulo State Univ Unesp, Botucatu Sch Med, Dept Clin Med, Botucatu, SP, BrazilSão Paulo State Univ Unesp, Botucatu Sch Med, Dept Biostat, Botucatu, SP, BrazilSão Paulo State Univ Unesp, Botucatu Sch Med, Dept Clin Med, Botucatu, SP, BrazilSão Paulo State Univ Unesp, Botucatu Sch Med, Dept Biostat, Botucatu, SP, BrazilSbem-soc Brasil Endocrinologia & MetabologiaUniversidade Estadual Paulista (Unesp)Saraiva, Patricia R. [UNESP]Teixeira, Silvania S. [UNESP]Nogueira, Célia Regina [UNESP]Padovani, Carlos Roberto [UNESP]2014-05-20T13:33:17Z2014-05-20T13:33:17Z2008-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article109-113application/pdfhttp://dx.doi.org/10.1590/S0004-27302008000100015Arquivos Brasileiros de Endocrinologia E Metabologia. Rio de Janeiro, Rj: Sbem-soc Brasil Endocrinologia & Metabologia, v. 52, n. 1, p. 109-113, 2008.0004-2730http://hdl.handle.net/11449/11377S0004-27302008000100015WOS:000256344600015S0004-27302008000100015-en.pdf8727897080522289Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos Brasileiros de Endocrinologia & Metabologia1.515info:eu-repo/semantics/openAccess2024-01-11T06:33:13Zoai:repositorio.unesp.br:11449/11377Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-11T06:33:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells
Triiodotironina (T3) não induz a expressão de Rankl em células de rato ROS 17/2.8
title Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells
spellingShingle Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells
Saraiva, Patricia R. [UNESP]
thyroid hormone
RANKL
bone
rat
ROS17/2.8 cell
title_short Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells
title_full Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells
title_fullStr Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells
title_full_unstemmed Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells
title_sort Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells
author Saraiva, Patricia R. [UNESP]
author_facet Saraiva, Patricia R. [UNESP]
Teixeira, Silvania S. [UNESP]
Nogueira, Célia Regina [UNESP]
Padovani, Carlos Roberto [UNESP]
author_role author
author2 Teixeira, Silvania S. [UNESP]
Nogueira, Célia Regina [UNESP]
Padovani, Carlos Roberto [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Saraiva, Patricia R. [UNESP]
Teixeira, Silvania S. [UNESP]
Nogueira, Célia Regina [UNESP]
Padovani, Carlos Roberto [UNESP]
dc.subject.por.fl_str_mv thyroid hormone
RANKL
bone
rat
ROS17/2.8 cell
topic thyroid hormone
RANKL
bone
rat
ROS17/2.8 cell
description Osteoclastogenesis may be regulated via activation of the RANK/RANKL (receptor activator of nuclear factor-kappa B/ receptor activator of nuclear factor-kappa B ligand) system, which is mediated by osteoblasts. However, the bone loss mechanism induced by T3 (triiodothyronine) is still controversial. In this study, osteoblastic lineage rat cells (ROS 17/2.8) were treated with T3 (10(-8) M 10(-9) 10 M, and 10(-10) M), and RANKL mRNA (messenger RNA) expression was measured by semiquantitative RT-PCR. Our results show that T3 concentrations used did not significantly enhance RANKL expression compared to controls without hormone treatment. This data suggests that other mechanisms, unrelated to the RANK/RANKL system, might be to activate osteoclast differentiation in these cells.
publishDate 2008
dc.date.none.fl_str_mv 2008-02-01
2014-05-20T13:33:17Z
2014-05-20T13:33:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0004-27302008000100015
Arquivos Brasileiros de Endocrinologia E Metabologia. Rio de Janeiro, Rj: Sbem-soc Brasil Endocrinologia & Metabologia, v. 52, n. 1, p. 109-113, 2008.
0004-2730
http://hdl.handle.net/11449/11377
S0004-27302008000100015
WOS:000256344600015
S0004-27302008000100015-en.pdf
8727897080522289
url http://dx.doi.org/10.1590/S0004-27302008000100015
http://hdl.handle.net/11449/11377
identifier_str_mv Arquivos Brasileiros de Endocrinologia E Metabologia. Rio de Janeiro, Rj: Sbem-soc Brasil Endocrinologia & Metabologia, v. 52, n. 1, p. 109-113, 2008.
0004-2730
S0004-27302008000100015
WOS:000256344600015
S0004-27302008000100015-en.pdf
8727897080522289
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros de Endocrinologia & Metabologia
1.515
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 109-113
application/pdf
dc.publisher.none.fl_str_mv Sbem-soc Brasil Endocrinologia & Metabologia
publisher.none.fl_str_mv Sbem-soc Brasil Endocrinologia & Metabologia
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965590551527424

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