Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0004-27302008000100015 http://hdl.handle.net/11449/11377 |
Resumo: | Osteoclastogenesis may be regulated via activation of the RANK/RANKL (receptor activator of nuclear factor-kappa B/ receptor activator of nuclear factor-kappa B ligand) system, which is mediated by osteoblasts. However, the bone loss mechanism induced by T3 (triiodothyronine) is still controversial. In this study, osteoblastic lineage rat cells (ROS 17/2.8) were treated with T3 (10(-8) M 10(-9) 10 M, and 10(-10) M), and RANKL mRNA (messenger RNA) expression was measured by semiquantitative RT-PCR. Our results show that T3 concentrations used did not significantly enhance RANKL expression compared to controls without hormone treatment. This data suggests that other mechanisms, unrelated to the RANK/RANKL system, might be to activate osteoclast differentiation in these cells. |
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Repositório Institucional da UNESP |
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Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cellsTriiodotironina (T3) não induz a expressão de Rankl em células de rato ROS 17/2.8thyroid hormoneRANKLboneratROS17/2.8 cellOsteoclastogenesis may be regulated via activation of the RANK/RANKL (receptor activator of nuclear factor-kappa B/ receptor activator of nuclear factor-kappa B ligand) system, which is mediated by osteoblasts. However, the bone loss mechanism induced by T3 (triiodothyronine) is still controversial. In this study, osteoblastic lineage rat cells (ROS 17/2.8) were treated with T3 (10(-8) M 10(-9) 10 M, and 10(-10) M), and RANKL mRNA (messenger RNA) expression was measured by semiquantitative RT-PCR. Our results show that T3 concentrations used did not significantly enhance RANKL expression compared to controls without hormone treatment. This data suggests that other mechanisms, unrelated to the RANK/RANKL system, might be to activate osteoclast differentiation in these cells.A osteoclastogênese pode ser regulada via ativação do sistema RANK/RANKL (receptor ativador do fator nuclear kapa B/ ligante do receptor do fator nuclear kapa B), que é mediado pelos osteoblastos. Entretanto, o mecanismo de perda óssea induzido pelo T3 (triiodotironina) ainda é controverso. Neste estudo, a linhagem osteoblástica de células de rato ROS 17/2.8 foi tratada com T3 (10-8 M, 10-9 M e 10-10 M), e a expressão do mRNA do RANKL foi medida por RT-PCR semiquantitativo. Nossos resultados mostraram que as concentrações de T3 utilizadas não induziram significativamente a expressão do RANKL, comparado ao controle (sem tratamento hormonal). Estes dados sugerem que outros mecanismos, não relacionados ao sistema RANK/RANKL, são usados para ativar a diferenciação osteoclástica nestas células.São Paulo State Univ Unesp, Botucatu Sch Med, Dept Clin Med, Botucatu, SP, BrazilSão Paulo State Univ Unesp, Botucatu Sch Med, Dept Biostat, Botucatu, SP, BrazilSão Paulo State Univ Unesp, Botucatu Sch Med, Dept Clin Med, Botucatu, SP, BrazilSão Paulo State Univ Unesp, Botucatu Sch Med, Dept Biostat, Botucatu, SP, BrazilSbem-soc Brasil Endocrinologia & MetabologiaUniversidade Estadual Paulista (Unesp)Saraiva, Patricia R. [UNESP]Teixeira, Silvania S. [UNESP]Nogueira, Célia Regina [UNESP]Padovani, Carlos Roberto [UNESP]2014-05-20T13:33:17Z2014-05-20T13:33:17Z2008-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article109-113application/pdfhttp://dx.doi.org/10.1590/S0004-27302008000100015Arquivos Brasileiros de Endocrinologia E Metabologia. Rio de Janeiro, Rj: Sbem-soc Brasil Endocrinologia & Metabologia, v. 52, n. 1, p. 109-113, 2008.0004-2730http://hdl.handle.net/11449/11377S0004-27302008000100015WOS:000256344600015S0004-27302008000100015-en.pdf8727897080522289Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos Brasileiros de Endocrinologia & Metabologia1.515info:eu-repo/semantics/openAccess2024-08-14T17:36:43Zoai:repositorio.unesp.br:11449/11377Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:36:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells Triiodotironina (T3) não induz a expressão de Rankl em células de rato ROS 17/2.8 |
title |
Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells |
spellingShingle |
Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells Saraiva, Patricia R. [UNESP] thyroid hormone RANKL bone rat ROS17/2.8 cell |
title_short |
Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells |
title_full |
Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells |
title_fullStr |
Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells |
title_full_unstemmed |
Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells |
title_sort |
Triiodothyronine (T3) does not induce RANKL expression in rat ROS 17/2.8 cells |
author |
Saraiva, Patricia R. [UNESP] |
author_facet |
Saraiva, Patricia R. [UNESP] Teixeira, Silvania S. [UNESP] Nogueira, Célia Regina [UNESP] Padovani, Carlos Roberto [UNESP] |
author_role |
author |
author2 |
Teixeira, Silvania S. [UNESP] Nogueira, Célia Regina [UNESP] Padovani, Carlos Roberto [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Saraiva, Patricia R. [UNESP] Teixeira, Silvania S. [UNESP] Nogueira, Célia Regina [UNESP] Padovani, Carlos Roberto [UNESP] |
dc.subject.por.fl_str_mv |
thyroid hormone RANKL bone rat ROS17/2.8 cell |
topic |
thyroid hormone RANKL bone rat ROS17/2.8 cell |
description |
Osteoclastogenesis may be regulated via activation of the RANK/RANKL (receptor activator of nuclear factor-kappa B/ receptor activator of nuclear factor-kappa B ligand) system, which is mediated by osteoblasts. However, the bone loss mechanism induced by T3 (triiodothyronine) is still controversial. In this study, osteoblastic lineage rat cells (ROS 17/2.8) were treated with T3 (10(-8) M 10(-9) 10 M, and 10(-10) M), and RANKL mRNA (messenger RNA) expression was measured by semiquantitative RT-PCR. Our results show that T3 concentrations used did not significantly enhance RANKL expression compared to controls without hormone treatment. This data suggests that other mechanisms, unrelated to the RANK/RANKL system, might be to activate osteoclast differentiation in these cells. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-02-01 2014-05-20T13:33:17Z 2014-05-20T13:33:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0004-27302008000100015 Arquivos Brasileiros de Endocrinologia E Metabologia. Rio de Janeiro, Rj: Sbem-soc Brasil Endocrinologia & Metabologia, v. 52, n. 1, p. 109-113, 2008. 0004-2730 http://hdl.handle.net/11449/11377 S0004-27302008000100015 WOS:000256344600015 S0004-27302008000100015-en.pdf 8727897080522289 |
url |
http://dx.doi.org/10.1590/S0004-27302008000100015 http://hdl.handle.net/11449/11377 |
identifier_str_mv |
Arquivos Brasileiros de Endocrinologia E Metabologia. Rio de Janeiro, Rj: Sbem-soc Brasil Endocrinologia & Metabologia, v. 52, n. 1, p. 109-113, 2008. 0004-2730 S0004-27302008000100015 WOS:000256344600015 S0004-27302008000100015-en.pdf 8727897080522289 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Arquivos Brasileiros de Endocrinologia & Metabologia 1.515 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
109-113 application/pdf |
dc.publisher.none.fl_str_mv |
Sbem-soc Brasil Endocrinologia & Metabologia |
publisher.none.fl_str_mv |
Sbem-soc Brasil Endocrinologia & Metabologia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128200064106496 |