The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions

Detalhes bibliográficos
Autor(a) principal: Camacho, Sabrina A. [UNESP]
Data de Publicação: 2022
Outros Autores: Kobal, Mirella B. [UNESP], Moreira, Lucas G. [UNESP], Bistaffa, Maria J. [UNESP], Roque, Thamires C. [UNESP], Pazin, Wallance M. [UNESP], Toledo, Karina A. [UNESP], Oliveira, Osvaldo N. [UNESP], Aoki, Pedro H.B. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.colsurfb.2021.112301
http://hdl.handle.net/11449/223124
Resumo: Photoinduced hyperthermia with nanomaterials has been proven effective in photothermal therapy (PTT) of tumor tissues, but a precise control in PTT requires determination of the molecular-level mechanisms. In this paper, we determined the mechanisms responsible for the action of photoexcited gold shell-isolated nanoparticles (AuSHINs) in reducing the viability of MCF7 (glandular breast cancer) and especially A549 (lung adenocarcinoma) cells in vitro experiments, while the photoinduced damage to healthy cells was much smaller. The photoinduced effects were more significant than using other nanomaterials, and could be explained by the different effects from incorporating AuSHINs on Langmuir monolayers from lipid extracts of tumoral (MCF7 and A549) and healthy cells. The incorporation of AuSHINs caused similar expansion of the Langmuir monolayers, but Fourier-transform infrared spectroscopy (FTIR) data of Langmuir-Schaefer films (LS) indicated distinct levels of penetration into the monolayers. AuSHINs penetrated deeper into the A549 extract monolayers, affecting the vibrational modes of polar groups and carbon chains, while in MCF7 monolayers penetration was limited to the surroundings of the polar groups. Even smaller insertion was observed for monolayers of the healthy cell extract. The photochemical reactions were modulated by AuSHINs penetration, since upon irradiation the surface area of A549 monolayer decreased owing to lipid chain cleavage by oxidative reactions. For MCF7 monolayers, hydroperoxidation under illumination led to a ca. 5% increase in surface area. The monolayers of healthy cell lipid extract were barely affected by irradiation, consistent with the lowest degree of AuSHINs insertion. In summary, efficient photothermal therapy may be devised by producing AuSHINs capable of penetrating the chain region of tumor cell membranes.
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spelling The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactionsGold shell-isolated nanoparticlesLangmuir monolayersPhotoinduced hyperthermiaTumoral and healthy cellsPhotoinduced hyperthermia with nanomaterials has been proven effective in photothermal therapy (PTT) of tumor tissues, but a precise control in PTT requires determination of the molecular-level mechanisms. In this paper, we determined the mechanisms responsible for the action of photoexcited gold shell-isolated nanoparticles (AuSHINs) in reducing the viability of MCF7 (glandular breast cancer) and especially A549 (lung adenocarcinoma) cells in vitro experiments, while the photoinduced damage to healthy cells was much smaller. The photoinduced effects were more significant than using other nanomaterials, and could be explained by the different effects from incorporating AuSHINs on Langmuir monolayers from lipid extracts of tumoral (MCF7 and A549) and healthy cells. The incorporation of AuSHINs caused similar expansion of the Langmuir monolayers, but Fourier-transform infrared spectroscopy (FTIR) data of Langmuir-Schaefer films (LS) indicated distinct levels of penetration into the monolayers. AuSHINs penetrated deeper into the A549 extract monolayers, affecting the vibrational modes of polar groups and carbon chains, while in MCF7 monolayers penetration was limited to the surroundings of the polar groups. Even smaller insertion was observed for monolayers of the healthy cell extract. The photochemical reactions were modulated by AuSHINs penetration, since upon irradiation the surface area of A549 monolayer decreased owing to lipid chain cleavage by oxidative reactions. For MCF7 monolayers, hydroperoxidation under illumination led to a ca. 5% increase in surface area. The monolayers of healthy cell lipid extract were barely affected by irradiation, consistent with the lowest degree of AuSHINs insertion. In summary, efficient photothermal therapy may be devised by producing AuSHINs capable of penetrating the chain region of tumor cell membranes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) School of Sciences Humanities and LanguagesIFSC São Carlos Institute of Physics University of São Paulo (USP)São Paulo State University (UNESP) Institute of Biosciences Letters and Exact SciencesSão Paulo State University (UNESP) School of Technology and Applied SciencesSão Paulo State University (UNESP) School of Sciences Humanities and LanguagesSão Paulo State University (UNESP) Institute of Biosciences Letters and Exact SciencesSão Paulo State University (UNESP) School of Technology and Applied SciencesFAPESP: 2018/14692-5FAPESP: 2020/12129-1FAPESP: 2020/15854-9Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Camacho, Sabrina A. [UNESP]Kobal, Mirella B. [UNESP]Moreira, Lucas G. [UNESP]Bistaffa, Maria J. [UNESP]Roque, Thamires C. [UNESP]Pazin, Wallance M. [UNESP]Toledo, Karina A. [UNESP]Oliveira, Osvaldo N. [UNESP]Aoki, Pedro H.B. [UNESP]2022-04-28T19:48:48Z2022-04-28T19:48:48Z2022-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.colsurfb.2021.112301Colloids and Surfaces B: Biointerfaces, v. 211.1873-43670927-7765http://hdl.handle.net/11449/22312410.1016/j.colsurfb.2021.1123012-s2.0-85121967378Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengColloids and Surfaces B: Biointerfacesinfo:eu-repo/semantics/openAccess2022-04-28T19:48:48Zoai:repositorio.unesp.br:11449/223124Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:48:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions
title The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions
spellingShingle The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions
Camacho, Sabrina A. [UNESP]
Gold shell-isolated nanoparticles
Langmuir monolayers
Photoinduced hyperthermia
Tumoral and healthy cells
title_short The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions
title_full The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions
title_fullStr The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions
title_full_unstemmed The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions
title_sort The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions
author Camacho, Sabrina A. [UNESP]
author_facet Camacho, Sabrina A. [UNESP]
Kobal, Mirella B. [UNESP]
Moreira, Lucas G. [UNESP]
Bistaffa, Maria J. [UNESP]
Roque, Thamires C. [UNESP]
Pazin, Wallance M. [UNESP]
Toledo, Karina A. [UNESP]
Oliveira, Osvaldo N. [UNESP]
Aoki, Pedro H.B. [UNESP]
author_role author
author2 Kobal, Mirella B. [UNESP]
Moreira, Lucas G. [UNESP]
Bistaffa, Maria J. [UNESP]
Roque, Thamires C. [UNESP]
Pazin, Wallance M. [UNESP]
Toledo, Karina A. [UNESP]
Oliveira, Osvaldo N. [UNESP]
Aoki, Pedro H.B. [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Camacho, Sabrina A. [UNESP]
Kobal, Mirella B. [UNESP]
Moreira, Lucas G. [UNESP]
Bistaffa, Maria J. [UNESP]
Roque, Thamires C. [UNESP]
Pazin, Wallance M. [UNESP]
Toledo, Karina A. [UNESP]
Oliveira, Osvaldo N. [UNESP]
Aoki, Pedro H.B. [UNESP]
dc.subject.por.fl_str_mv Gold shell-isolated nanoparticles
Langmuir monolayers
Photoinduced hyperthermia
Tumoral and healthy cells
topic Gold shell-isolated nanoparticles
Langmuir monolayers
Photoinduced hyperthermia
Tumoral and healthy cells
description Photoinduced hyperthermia with nanomaterials has been proven effective in photothermal therapy (PTT) of tumor tissues, but a precise control in PTT requires determination of the molecular-level mechanisms. In this paper, we determined the mechanisms responsible for the action of photoexcited gold shell-isolated nanoparticles (AuSHINs) in reducing the viability of MCF7 (glandular breast cancer) and especially A549 (lung adenocarcinoma) cells in vitro experiments, while the photoinduced damage to healthy cells was much smaller. The photoinduced effects were more significant than using other nanomaterials, and could be explained by the different effects from incorporating AuSHINs on Langmuir monolayers from lipid extracts of tumoral (MCF7 and A549) and healthy cells. The incorporation of AuSHINs caused similar expansion of the Langmuir monolayers, but Fourier-transform infrared spectroscopy (FTIR) data of Langmuir-Schaefer films (LS) indicated distinct levels of penetration into the monolayers. AuSHINs penetrated deeper into the A549 extract monolayers, affecting the vibrational modes of polar groups and carbon chains, while in MCF7 monolayers penetration was limited to the surroundings of the polar groups. Even smaller insertion was observed for monolayers of the healthy cell extract. The photochemical reactions were modulated by AuSHINs penetration, since upon irradiation the surface area of A549 monolayer decreased owing to lipid chain cleavage by oxidative reactions. For MCF7 monolayers, hydroperoxidation under illumination led to a ca. 5% increase in surface area. The monolayers of healthy cell lipid extract were barely affected by irradiation, consistent with the lowest degree of AuSHINs insertion. In summary, efficient photothermal therapy may be devised by producing AuSHINs capable of penetrating the chain region of tumor cell membranes.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-28T19:48:48Z
2022-04-28T19:48:48Z
2022-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.colsurfb.2021.112301
Colloids and Surfaces B: Biointerfaces, v. 211.
1873-4367
0927-7765
http://hdl.handle.net/11449/223124
10.1016/j.colsurfb.2021.112301
2-s2.0-85121967378
url http://dx.doi.org/10.1016/j.colsurfb.2021.112301
http://hdl.handle.net/11449/223124
identifier_str_mv Colloids and Surfaces B: Biointerfaces, v. 211.
1873-4367
0927-7765
10.1016/j.colsurfb.2021.112301
2-s2.0-85121967378
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Colloids and Surfaces B: Biointerfaces
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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