The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.colsurfb.2021.112301 http://hdl.handle.net/11449/223124 |
Resumo: | Photoinduced hyperthermia with nanomaterials has been proven effective in photothermal therapy (PTT) of tumor tissues, but a precise control in PTT requires determination of the molecular-level mechanisms. In this paper, we determined the mechanisms responsible for the action of photoexcited gold shell-isolated nanoparticles (AuSHINs) in reducing the viability of MCF7 (glandular breast cancer) and especially A549 (lung adenocarcinoma) cells in vitro experiments, while the photoinduced damage to healthy cells was much smaller. The photoinduced effects were more significant than using other nanomaterials, and could be explained by the different effects from incorporating AuSHINs on Langmuir monolayers from lipid extracts of tumoral (MCF7 and A549) and healthy cells. The incorporation of AuSHINs caused similar expansion of the Langmuir monolayers, but Fourier-transform infrared spectroscopy (FTIR) data of Langmuir-Schaefer films (LS) indicated distinct levels of penetration into the monolayers. AuSHINs penetrated deeper into the A549 extract monolayers, affecting the vibrational modes of polar groups and carbon chains, while in MCF7 monolayers penetration was limited to the surroundings of the polar groups. Even smaller insertion was observed for monolayers of the healthy cell extract. The photochemical reactions were modulated by AuSHINs penetration, since upon irradiation the surface area of A549 monolayer decreased owing to lipid chain cleavage by oxidative reactions. For MCF7 monolayers, hydroperoxidation under illumination led to a ca. 5% increase in surface area. The monolayers of healthy cell lipid extract were barely affected by irradiation, consistent with the lowest degree of AuSHINs insertion. In summary, efficient photothermal therapy may be devised by producing AuSHINs capable of penetrating the chain region of tumor cell membranes. |
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The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactionsGold shell-isolated nanoparticlesLangmuir monolayersPhotoinduced hyperthermiaTumoral and healthy cellsPhotoinduced hyperthermia with nanomaterials has been proven effective in photothermal therapy (PTT) of tumor tissues, but a precise control in PTT requires determination of the molecular-level mechanisms. In this paper, we determined the mechanisms responsible for the action of photoexcited gold shell-isolated nanoparticles (AuSHINs) in reducing the viability of MCF7 (glandular breast cancer) and especially A549 (lung adenocarcinoma) cells in vitro experiments, while the photoinduced damage to healthy cells was much smaller. The photoinduced effects were more significant than using other nanomaterials, and could be explained by the different effects from incorporating AuSHINs on Langmuir monolayers from lipid extracts of tumoral (MCF7 and A549) and healthy cells. The incorporation of AuSHINs caused similar expansion of the Langmuir monolayers, but Fourier-transform infrared spectroscopy (FTIR) data of Langmuir-Schaefer films (LS) indicated distinct levels of penetration into the monolayers. AuSHINs penetrated deeper into the A549 extract monolayers, affecting the vibrational modes of polar groups and carbon chains, while in MCF7 monolayers penetration was limited to the surroundings of the polar groups. Even smaller insertion was observed for monolayers of the healthy cell extract. The photochemical reactions were modulated by AuSHINs penetration, since upon irradiation the surface area of A549 monolayer decreased owing to lipid chain cleavage by oxidative reactions. For MCF7 monolayers, hydroperoxidation under illumination led to a ca. 5% increase in surface area. The monolayers of healthy cell lipid extract were barely affected by irradiation, consistent with the lowest degree of AuSHINs insertion. In summary, efficient photothermal therapy may be devised by producing AuSHINs capable of penetrating the chain region of tumor cell membranes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) School of Sciences Humanities and LanguagesIFSC São Carlos Institute of Physics University of São Paulo (USP)São Paulo State University (UNESP) Institute of Biosciences Letters and Exact SciencesSão Paulo State University (UNESP) School of Technology and Applied SciencesSão Paulo State University (UNESP) School of Sciences Humanities and LanguagesSão Paulo State University (UNESP) Institute of Biosciences Letters and Exact SciencesSão Paulo State University (UNESP) School of Technology and Applied SciencesFAPESP: 2018/14692-5FAPESP: 2020/12129-1FAPESP: 2020/15854-9Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Camacho, Sabrina A. [UNESP]Kobal, Mirella B. [UNESP]Moreira, Lucas G. [UNESP]Bistaffa, Maria J. [UNESP]Roque, Thamires C. [UNESP]Pazin, Wallance M. [UNESP]Toledo, Karina A. [UNESP]Oliveira, Osvaldo N. [UNESP]Aoki, Pedro H.B. [UNESP]2022-04-28T19:48:48Z2022-04-28T19:48:48Z2022-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.colsurfb.2021.112301Colloids and Surfaces B: Biointerfaces, v. 211.1873-43670927-7765http://hdl.handle.net/11449/22312410.1016/j.colsurfb.2021.1123012-s2.0-85121967378Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengColloids and Surfaces B: Biointerfacesinfo:eu-repo/semantics/openAccess2022-04-28T19:48:48Zoai:repositorio.unesp.br:11449/223124Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:12:01.782386Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions |
title |
The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions |
spellingShingle |
The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions Camacho, Sabrina A. [UNESP] Gold shell-isolated nanoparticles Langmuir monolayers Photoinduced hyperthermia Tumoral and healthy cells |
title_short |
The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions |
title_full |
The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions |
title_fullStr |
The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions |
title_full_unstemmed |
The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions |
title_sort |
The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions |
author |
Camacho, Sabrina A. [UNESP] |
author_facet |
Camacho, Sabrina A. [UNESP] Kobal, Mirella B. [UNESP] Moreira, Lucas G. [UNESP] Bistaffa, Maria J. [UNESP] Roque, Thamires C. [UNESP] Pazin, Wallance M. [UNESP] Toledo, Karina A. [UNESP] Oliveira, Osvaldo N. [UNESP] Aoki, Pedro H.B. [UNESP] |
author_role |
author |
author2 |
Kobal, Mirella B. [UNESP] Moreira, Lucas G. [UNESP] Bistaffa, Maria J. [UNESP] Roque, Thamires C. [UNESP] Pazin, Wallance M. [UNESP] Toledo, Karina A. [UNESP] Oliveira, Osvaldo N. [UNESP] Aoki, Pedro H.B. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Camacho, Sabrina A. [UNESP] Kobal, Mirella B. [UNESP] Moreira, Lucas G. [UNESP] Bistaffa, Maria J. [UNESP] Roque, Thamires C. [UNESP] Pazin, Wallance M. [UNESP] Toledo, Karina A. [UNESP] Oliveira, Osvaldo N. [UNESP] Aoki, Pedro H.B. [UNESP] |
dc.subject.por.fl_str_mv |
Gold shell-isolated nanoparticles Langmuir monolayers Photoinduced hyperthermia Tumoral and healthy cells |
topic |
Gold shell-isolated nanoparticles Langmuir monolayers Photoinduced hyperthermia Tumoral and healthy cells |
description |
Photoinduced hyperthermia with nanomaterials has been proven effective in photothermal therapy (PTT) of tumor tissues, but a precise control in PTT requires determination of the molecular-level mechanisms. In this paper, we determined the mechanisms responsible for the action of photoexcited gold shell-isolated nanoparticles (AuSHINs) in reducing the viability of MCF7 (glandular breast cancer) and especially A549 (lung adenocarcinoma) cells in vitro experiments, while the photoinduced damage to healthy cells was much smaller. The photoinduced effects were more significant than using other nanomaterials, and could be explained by the different effects from incorporating AuSHINs on Langmuir monolayers from lipid extracts of tumoral (MCF7 and A549) and healthy cells. The incorporation of AuSHINs caused similar expansion of the Langmuir monolayers, but Fourier-transform infrared spectroscopy (FTIR) data of Langmuir-Schaefer films (LS) indicated distinct levels of penetration into the monolayers. AuSHINs penetrated deeper into the A549 extract monolayers, affecting the vibrational modes of polar groups and carbon chains, while in MCF7 monolayers penetration was limited to the surroundings of the polar groups. Even smaller insertion was observed for monolayers of the healthy cell extract. The photochemical reactions were modulated by AuSHINs penetration, since upon irradiation the surface area of A549 monolayer decreased owing to lipid chain cleavage by oxidative reactions. For MCF7 monolayers, hydroperoxidation under illumination led to a ca. 5% increase in surface area. The monolayers of healthy cell lipid extract were barely affected by irradiation, consistent with the lowest degree of AuSHINs insertion. In summary, efficient photothermal therapy may be devised by producing AuSHINs capable of penetrating the chain region of tumor cell membranes. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:48:48Z 2022-04-28T19:48:48Z 2022-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.colsurfb.2021.112301 Colloids and Surfaces B: Biointerfaces, v. 211. 1873-4367 0927-7765 http://hdl.handle.net/11449/223124 10.1016/j.colsurfb.2021.112301 2-s2.0-85121967378 |
url |
http://dx.doi.org/10.1016/j.colsurfb.2021.112301 http://hdl.handle.net/11449/223124 |
identifier_str_mv |
Colloids and Surfaces B: Biointerfaces, v. 211. 1873-4367 0927-7765 10.1016/j.colsurfb.2021.112301 2-s2.0-85121967378 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Colloids and Surfaces B: Biointerfaces |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129297742823424 |