Marcadores de estresse oxidativo da saliva de cães com doença periodontal
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/144068 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/08-09-2016/000870640.pdf |
Resumo: | Periodontal disease (PD) is the most common disease of the oral cavity in dogs with an inflammatory character disease, chronic and infectious. With an excessive production of reactive oxygen species, oxidative stress occurs which is an imbalance between oxidants and antioxidants. Human saliva is considered the first line of defense against oxidative stress in periodontal diseases. Due to lack of studies in dogs on this issue, this study hypotheses were investigated that the similarity to what is described in humans, the DP in dogs cause systemic oxidative stress associated with activation of neutrophils and that saliva can be used to assess this oxidative stress. We selected 20 adult dogs of different breeds and sex, grouped according to the degree of injury (n-6 gingivitis, periodontitis light n-8 and advanced n-6). The control group was made up of the same 20 dogs, 30 days after periodontal treatment, all without any changes on physical examination, serum chemistry profile and complete blood count. To assess oxidative metabolism of circulating neutrophils was measured superoxide production by cytochemical test nitroblue tetrazolium reduction (NBT), and total oxidant concentration (TOC). The concentration of the tiobartbitúrico reactive species (TBARS) in plasma was used to estimate the lipid peroxidation. The local oxidative stress was evaluated by quantifying the TOC and concentration of the main antioxidant in saliva (uric acid, albumin and bilirubin). The systemic oxidative stress in PD has occurred regardless of the degree of injury and is characterized by TOC increase, NBT and TBARS. Changes in oxidative stress biomarkers measured in saliva were not significant and did not show correlation with the values obtained in plasma. Contrary to our hypothesis, the dog saliva analysis does not clearly expressed oxidative stress caused by PD |
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Marcadores de estresse oxidativo da saliva de cães com doença periodontalCãesDoenças periodontaisAntioxidantesSalivaAgentes oxidantesPeriodontiteInflamaçãoNeutrófilosPeriodontitisPeriodontal disease (PD) is the most common disease of the oral cavity in dogs with an inflammatory character disease, chronic and infectious. With an excessive production of reactive oxygen species, oxidative stress occurs which is an imbalance between oxidants and antioxidants. Human saliva is considered the first line of defense against oxidative stress in periodontal diseases. Due to lack of studies in dogs on this issue, this study hypotheses were investigated that the similarity to what is described in humans, the DP in dogs cause systemic oxidative stress associated with activation of neutrophils and that saliva can be used to assess this oxidative stress. We selected 20 adult dogs of different breeds and sex, grouped according to the degree of injury (n-6 gingivitis, periodontitis light n-8 and advanced n-6). The control group was made up of the same 20 dogs, 30 days after periodontal treatment, all without any changes on physical examination, serum chemistry profile and complete blood count. To assess oxidative metabolism of circulating neutrophils was measured superoxide production by cytochemical test nitroblue tetrazolium reduction (NBT), and total oxidant concentration (TOC). The concentration of the tiobartbitúrico reactive species (TBARS) in plasma was used to estimate the lipid peroxidation. The local oxidative stress was evaluated by quantifying the TOC and concentration of the main antioxidant in saliva (uric acid, albumin and bilirubin). The systemic oxidative stress in PD has occurred regardless of the degree of injury and is characterized by TOC increase, NBT and TBARS. Changes in oxidative stress biomarkers measured in saliva were not significant and did not show correlation with the values obtained in plasma. Contrary to our hypothesis, the dog saliva analysis does not clearly expressed oxidative stress caused by PDCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista (Unesp)Ciarlini, Paulo César [UNESP]Universidade Estadual Paulista (Unesp)Silva, Mirtes Rosa da [UNESP]2016-09-27T13:40:02Z2016-09-27T13:40:02Z2015-01-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis56 f.application/pdfSILVA, Mirtes Rosa da. Marcadores de estresse oxidativo da saliva de cães com doença periodontal. 2015. 56 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária, 2015.http://hdl.handle.net/11449/144068000870640http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/08-09-2016/000870640.pdf33004021075P836139400182995000000-0003-1480-5208Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-10-31T06:08:26Zoai:repositorio.unesp.br:11449/144068Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:31:12.479923Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Marcadores de estresse oxidativo da saliva de cães com doença periodontal |
title |
Marcadores de estresse oxidativo da saliva de cães com doença periodontal |
spellingShingle |
Marcadores de estresse oxidativo da saliva de cães com doença periodontal Silva, Mirtes Rosa da [UNESP] Cães Doenças periodontais Antioxidantes Saliva Agentes oxidantes Periodontite Inflamação Neutrófilos Periodontitis |
title_short |
Marcadores de estresse oxidativo da saliva de cães com doença periodontal |
title_full |
Marcadores de estresse oxidativo da saliva de cães com doença periodontal |
title_fullStr |
Marcadores de estresse oxidativo da saliva de cães com doença periodontal |
title_full_unstemmed |
Marcadores de estresse oxidativo da saliva de cães com doença periodontal |
title_sort |
Marcadores de estresse oxidativo da saliva de cães com doença periodontal |
author |
Silva, Mirtes Rosa da [UNESP] |
author_facet |
Silva, Mirtes Rosa da [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Ciarlini, Paulo César [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, Mirtes Rosa da [UNESP] |
dc.subject.por.fl_str_mv |
Cães Doenças periodontais Antioxidantes Saliva Agentes oxidantes Periodontite Inflamação Neutrófilos Periodontitis |
topic |
Cães Doenças periodontais Antioxidantes Saliva Agentes oxidantes Periodontite Inflamação Neutrófilos Periodontitis |
description |
Periodontal disease (PD) is the most common disease of the oral cavity in dogs with an inflammatory character disease, chronic and infectious. With an excessive production of reactive oxygen species, oxidative stress occurs which is an imbalance between oxidants and antioxidants. Human saliva is considered the first line of defense against oxidative stress in periodontal diseases. Due to lack of studies in dogs on this issue, this study hypotheses were investigated that the similarity to what is described in humans, the DP in dogs cause systemic oxidative stress associated with activation of neutrophils and that saliva can be used to assess this oxidative stress. We selected 20 adult dogs of different breeds and sex, grouped according to the degree of injury (n-6 gingivitis, periodontitis light n-8 and advanced n-6). The control group was made up of the same 20 dogs, 30 days after periodontal treatment, all without any changes on physical examination, serum chemistry profile and complete blood count. To assess oxidative metabolism of circulating neutrophils was measured superoxide production by cytochemical test nitroblue tetrazolium reduction (NBT), and total oxidant concentration (TOC). The concentration of the tiobartbitúrico reactive species (TBARS) in plasma was used to estimate the lipid peroxidation. The local oxidative stress was evaluated by quantifying the TOC and concentration of the main antioxidant in saliva (uric acid, albumin and bilirubin). The systemic oxidative stress in PD has occurred regardless of the degree of injury and is characterized by TOC increase, NBT and TBARS. Changes in oxidative stress biomarkers measured in saliva were not significant and did not show correlation with the values obtained in plasma. Contrary to our hypothesis, the dog saliva analysis does not clearly expressed oxidative stress caused by PD |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-19 2016-09-27T13:40:02Z 2016-09-27T13:40:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SILVA, Mirtes Rosa da. Marcadores de estresse oxidativo da saliva de cães com doença periodontal. 2015. 56 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária, 2015. http://hdl.handle.net/11449/144068 000870640 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/08-09-2016/000870640.pdf 33004021075P8 3613940018299500 0000-0003-1480-5208 |
identifier_str_mv |
SILVA, Mirtes Rosa da. Marcadores de estresse oxidativo da saliva de cães com doença periodontal. 2015. 56 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária, 2015. 000870640 33004021075P8 3613940018299500 0000-0003-1480-5208 |
url |
http://hdl.handle.net/11449/144068 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/08-09-2016/000870640.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
56 f. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128665630801920 |