Use of nanoparticle concentration as a tool to understand the structural properties of colloids
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-017-18573-7 http://hdl.handle.net/11449/175841 |
Resumo: | Elucidation of the structural properties of colloids is paramount for a successful formulation. However, the intrinsic dynamism of colloidal systems makes their characterization a difficult task and, in particular, there is a lack of physicochemical techniques that can be correlated to their biological performance. Nanoparticle tracking analysis (NTA) allows measurements of size distribution and nanoparticle concentration in real time. Its analysis over time also enables the early detection of physical instability in the systems not assessed by subtle changes in size distribution. Nanoparticle concentration is a parameter with the potential to bridge the gap between in vitro characterization and biological performance of colloids, and therefore should be monitored in stability studies of formulations. To demonstrate this, we have followed two systems: extruded liposomes exposed to increasing CHCl3 concentrations, and solid lipid nanoparticles prepared with decreasing amounts of poloxamer 188. NTA and dynamic light scattering (DLS) were used to monitor changes in nanoparticle number and size, and to estimate the number of lipid components per particle. The results revealed a strong negative correlation between particle size (determined by DLS) and concentration (assessed by NTA) in diluted samples, which should be adopted to monitor nanocolloidal stability, especially in drug delivery. |
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Use of nanoparticle concentration as a tool to understand the structural properties of colloidsElucidation of the structural properties of colloids is paramount for a successful formulation. However, the intrinsic dynamism of colloidal systems makes their characterization a difficult task and, in particular, there is a lack of physicochemical techniques that can be correlated to their biological performance. Nanoparticle tracking analysis (NTA) allows measurements of size distribution and nanoparticle concentration in real time. Its analysis over time also enables the early detection of physical instability in the systems not assessed by subtle changes in size distribution. Nanoparticle concentration is a parameter with the potential to bridge the gap between in vitro characterization and biological performance of colloids, and therefore should be monitored in stability studies of formulations. To demonstrate this, we have followed two systems: extruded liposomes exposed to increasing CHCl3 concentrations, and solid lipid nanoparticles prepared with decreasing amounts of poloxamer 188. NTA and dynamic light scattering (DLS) were used to monitor changes in nanoparticle number and size, and to estimate the number of lipid components per particle. The results revealed a strong negative correlation between particle size (determined by DLS) and concentration (assessed by NTA) in diluted samples, which should be adopted to monitor nanocolloidal stability, especially in drug delivery.Department of Biochemistry and Tissue Biology Institute of Biology University of Campinas (UNICAMP)São Paulo State University (UNESP) Institute of Science and Technology of Sorocaba Laboratory of Environmental NanotechnologySão Paulo State University (UNESP) Institute of Science and Technology of Sorocaba Laboratory of Environmental NanotechnologyUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Ribeiro, Lígia Nunes De MoraisCouto, Verônica MunizFraceto, Leonardo Fernandes [UNESP]De Paula, Eneida2018-12-11T17:17:48Z2018-12-11T17:17:48Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1038/s41598-017-18573-7Scientific Reports, v. 8, n. 1, 2018.2045-2322http://hdl.handle.net/11449/17584110.1038/s41598-017-18573-72-s2.0-850415832202-s2.0-85041583220.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reports1,533info:eu-repo/semantics/openAccess2023-11-18T06:18:07Zoai:repositorio.unesp.br:11449/175841Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:06:07.892293Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of nanoparticle concentration as a tool to understand the structural properties of colloids |
title |
Use of nanoparticle concentration as a tool to understand the structural properties of colloids |
spellingShingle |
Use of nanoparticle concentration as a tool to understand the structural properties of colloids Ribeiro, Lígia Nunes De Morais |
title_short |
Use of nanoparticle concentration as a tool to understand the structural properties of colloids |
title_full |
Use of nanoparticle concentration as a tool to understand the structural properties of colloids |
title_fullStr |
Use of nanoparticle concentration as a tool to understand the structural properties of colloids |
title_full_unstemmed |
Use of nanoparticle concentration as a tool to understand the structural properties of colloids |
title_sort |
Use of nanoparticle concentration as a tool to understand the structural properties of colloids |
author |
Ribeiro, Lígia Nunes De Morais |
author_facet |
Ribeiro, Lígia Nunes De Morais Couto, Verônica Muniz Fraceto, Leonardo Fernandes [UNESP] De Paula, Eneida |
author_role |
author |
author2 |
Couto, Verônica Muniz Fraceto, Leonardo Fernandes [UNESP] De Paula, Eneida |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ribeiro, Lígia Nunes De Morais Couto, Verônica Muniz Fraceto, Leonardo Fernandes [UNESP] De Paula, Eneida |
description |
Elucidation of the structural properties of colloids is paramount for a successful formulation. However, the intrinsic dynamism of colloidal systems makes their characterization a difficult task and, in particular, there is a lack of physicochemical techniques that can be correlated to their biological performance. Nanoparticle tracking analysis (NTA) allows measurements of size distribution and nanoparticle concentration in real time. Its analysis over time also enables the early detection of physical instability in the systems not assessed by subtle changes in size distribution. Nanoparticle concentration is a parameter with the potential to bridge the gap between in vitro characterization and biological performance of colloids, and therefore should be monitored in stability studies of formulations. To demonstrate this, we have followed two systems: extruded liposomes exposed to increasing CHCl3 concentrations, and solid lipid nanoparticles prepared with decreasing amounts of poloxamer 188. NTA and dynamic light scattering (DLS) were used to monitor changes in nanoparticle number and size, and to estimate the number of lipid components per particle. The results revealed a strong negative correlation between particle size (determined by DLS) and concentration (assessed by NTA) in diluted samples, which should be adopted to monitor nanocolloidal stability, especially in drug delivery. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:17:48Z 2018-12-11T17:17:48Z 2018-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-017-18573-7 Scientific Reports, v. 8, n. 1, 2018. 2045-2322 http://hdl.handle.net/11449/175841 10.1038/s41598-017-18573-7 2-s2.0-85041583220 2-s2.0-85041583220.pdf |
url |
http://dx.doi.org/10.1038/s41598-017-18573-7 http://hdl.handle.net/11449/175841 |
identifier_str_mv |
Scientific Reports, v. 8, n. 1, 2018. 2045-2322 10.1038/s41598-017-18573-7 2-s2.0-85041583220 2-s2.0-85041583220.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports 1,533 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128894185766912 |