Mechanical, biochemical, and morphological changes in the heart from chronic food-restricted rats

Detalhes bibliográficos
Autor(a) principal: Okoshi, M. P. [UNESP]
Data de Publicação: 2001
Outros Autores: Okoshi, K. [UNESP], Pai, V. D. [UNESP], Pai-Silva, M. D. [UNESP], Matsubara, L. S. [UNESP], Cicogna, A. C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1139/y01-060
http://hdl.handle.net/11449/224191
Resumo: Food restriction (FR) has been shown to induce important morphological changes in rat myocardium. However, its influence on myocardial performance is not completely defined. We examined the effects of chronic FR on cardiac muscle function and morphology. Sixty-day-old Wistar-Kyoto rats were fed a control (C) or a restricted diet (daily intake reduced to 50% of the amount of food consumed by the control group) for 90 days. Myocardial performance was studied in isolated left ventricular (LV) papillary muscle. Fragments of the LV free wall were analysed by light microscopy, and the ultrastructure of the myocardium was examined in the LV papillary muscle. The myocardial collagen concentration was also evaluated. FR decreased body weight (BW) and LV weight (LVW); the LVW/BW ratio was higher in the restricted group (C, 1.86 ± 0.17 mg/g; FR, 2.19 ± 0.31 mg/g; p < 0.01). In the FR animals, the cardiac fibers were polymorphic, some of them were of small diameter and others presented lateral infoldings; the ultrastructural alterations were focal and included reduction of sarcoplasmic content, absence and (or) disorganization of myofilaments and Z line, numerous electron dense and polymorphic mitochondria, and deep infoldings of the plasma membrane. The hydroxyproline concentration was higher in the FR animals (p < 0.01). FR prolonged the contraction and relaxation time of the papillary muscle and did not change its ability to contract and shorten. In conclusion, although a 90-day period of FR caused striking myocardial ultrastructural alterations and increased the collagen concentration, it only minimally affected the mechanical function.
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spelling Mechanical, biochemical, and morphological changes in the heart from chronic food-restricted ratsDietMyocardiumPapillary muscleUltrastructureUndernutritionFood restriction (FR) has been shown to induce important morphological changes in rat myocardium. However, its influence on myocardial performance is not completely defined. We examined the effects of chronic FR on cardiac muscle function and morphology. Sixty-day-old Wistar-Kyoto rats were fed a control (C) or a restricted diet (daily intake reduced to 50% of the amount of food consumed by the control group) for 90 days. Myocardial performance was studied in isolated left ventricular (LV) papillary muscle. Fragments of the LV free wall were analysed by light microscopy, and the ultrastructure of the myocardium was examined in the LV papillary muscle. The myocardial collagen concentration was also evaluated. FR decreased body weight (BW) and LV weight (LVW); the LVW/BW ratio was higher in the restricted group (C, 1.86 ± 0.17 mg/g; FR, 2.19 ± 0.31 mg/g; p < 0.01). In the FR animals, the cardiac fibers were polymorphic, some of them were of small diameter and others presented lateral infoldings; the ultrastructural alterations were focal and included reduction of sarcoplasmic content, absence and (or) disorganization of myofilaments and Z line, numerous electron dense and polymorphic mitochondria, and deep infoldings of the plasma membrane. The hydroxyproline concentration was higher in the FR animals (p < 0.01). FR prolonged the contraction and relaxation time of the papillary muscle and did not change its ability to contract and shorten. In conclusion, although a 90-day period of FR caused striking myocardial ultrastructural alterations and increased the collagen concentration, it only minimally affected the mechanical function.Departamento de Clinica Medica Faculdade de Medicina de Botucatu UNESP, Rubião Júnior, S/N, CEP 18.618-000 Botucatu, Sao PauloDepartamento de Clinica Medica Faculdade de Medicina de Botucatu UNESP, Rubião Júnior, S/N, CEP 18.618-000 Botucatu, Sao PauloUniversidade Estadual Paulista (UNESP)Okoshi, M. P. [UNESP]Okoshi, K. [UNESP]Pai, V. D. [UNESP]Pai-Silva, M. D. [UNESP]Matsubara, L. S. [UNESP]Cicogna, A. C. [UNESP]2022-04-28T19:55:05Z2022-04-28T19:55:05Z2001-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article754-760http://dx.doi.org/10.1139/y01-060Canadian Journal of Physiology and Pharmacology, v. 79, n. 9, p. 754-760, 2001.0008-4212http://hdl.handle.net/11449/22419110.1139/y01-0602-s2.0-0034756528Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCanadian Journal of Physiology and Pharmacologyinfo:eu-repo/semantics/openAccess2024-08-14T17:23:09Zoai:repositorio.unesp.br:11449/224191Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mechanical, biochemical, and morphological changes in the heart from chronic food-restricted rats
title Mechanical, biochemical, and morphological changes in the heart from chronic food-restricted rats
spellingShingle Mechanical, biochemical, and morphological changes in the heart from chronic food-restricted rats
Okoshi, M. P. [UNESP]
Diet
Myocardium
Papillary muscle
Ultrastructure
Undernutrition
title_short Mechanical, biochemical, and morphological changes in the heart from chronic food-restricted rats
title_full Mechanical, biochemical, and morphological changes in the heart from chronic food-restricted rats
title_fullStr Mechanical, biochemical, and morphological changes in the heart from chronic food-restricted rats
title_full_unstemmed Mechanical, biochemical, and morphological changes in the heart from chronic food-restricted rats
title_sort Mechanical, biochemical, and morphological changes in the heart from chronic food-restricted rats
author Okoshi, M. P. [UNESP]
author_facet Okoshi, M. P. [UNESP]
Okoshi, K. [UNESP]
Pai, V. D. [UNESP]
Pai-Silva, M. D. [UNESP]
Matsubara, L. S. [UNESP]
Cicogna, A. C. [UNESP]
author_role author
author2 Okoshi, K. [UNESP]
Pai, V. D. [UNESP]
Pai-Silva, M. D. [UNESP]
Matsubara, L. S. [UNESP]
Cicogna, A. C. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Okoshi, M. P. [UNESP]
Okoshi, K. [UNESP]
Pai, V. D. [UNESP]
Pai-Silva, M. D. [UNESP]
Matsubara, L. S. [UNESP]
Cicogna, A. C. [UNESP]
dc.subject.por.fl_str_mv Diet
Myocardium
Papillary muscle
Ultrastructure
Undernutrition
topic Diet
Myocardium
Papillary muscle
Ultrastructure
Undernutrition
description Food restriction (FR) has been shown to induce important morphological changes in rat myocardium. However, its influence on myocardial performance is not completely defined. We examined the effects of chronic FR on cardiac muscle function and morphology. Sixty-day-old Wistar-Kyoto rats were fed a control (C) or a restricted diet (daily intake reduced to 50% of the amount of food consumed by the control group) for 90 days. Myocardial performance was studied in isolated left ventricular (LV) papillary muscle. Fragments of the LV free wall were analysed by light microscopy, and the ultrastructure of the myocardium was examined in the LV papillary muscle. The myocardial collagen concentration was also evaluated. FR decreased body weight (BW) and LV weight (LVW); the LVW/BW ratio was higher in the restricted group (C, 1.86 ± 0.17 mg/g; FR, 2.19 ± 0.31 mg/g; p < 0.01). In the FR animals, the cardiac fibers were polymorphic, some of them were of small diameter and others presented lateral infoldings; the ultrastructural alterations were focal and included reduction of sarcoplasmic content, absence and (or) disorganization of myofilaments and Z line, numerous electron dense and polymorphic mitochondria, and deep infoldings of the plasma membrane. The hydroxyproline concentration was higher in the FR animals (p < 0.01). FR prolonged the contraction and relaxation time of the papillary muscle and did not change its ability to contract and shorten. In conclusion, although a 90-day period of FR caused striking myocardial ultrastructural alterations and increased the collagen concentration, it only minimally affected the mechanical function.
publishDate 2001
dc.date.none.fl_str_mv 2001-01-01
2022-04-28T19:55:05Z
2022-04-28T19:55:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1139/y01-060
Canadian Journal of Physiology and Pharmacology, v. 79, n. 9, p. 754-760, 2001.
0008-4212
http://hdl.handle.net/11449/224191
10.1139/y01-060
2-s2.0-0034756528
url http://dx.doi.org/10.1139/y01-060
http://hdl.handle.net/11449/224191
identifier_str_mv Canadian Journal of Physiology and Pharmacology, v. 79, n. 9, p. 754-760, 2001.
0008-4212
10.1139/y01-060
2-s2.0-0034756528
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Canadian Journal of Physiology and Pharmacology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 754-760
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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