Citotoxicidade transamelodentinária de uma fita clareadora com 10% de peróxido de hidrogênio sobre células de linhagem odontoblástica

• Autor(a) principal: Pontes, Elaine C.V. [UNESP]
• Data de Publicação: 2012
• Outros Autores: Gomes, Livia R. M., Soares, Diana Gabriela de Souza [UNESP], Ribeiro, Ana Paula Dias [UNESP], Hebling, Josimeri [UNESP], Costa, Carlos Alberto de Souza [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://www.robrac.org.br/seer/index.php/ROBRAC/article/view/630; http://hdl.handle.net/11449/125709
• topic: Tooth bleaching; Toxicity; Odontoblasts; Clareamento dental; Toxicidade; Odontoblastos

To evaluate the cytotoxicity of a whitestrip (WS) with 10% hydrogen peroxide (HP) on cultured odontoblast-like cells. Material and Methods: Enamel/dentin discs were adapted to artificial pulp chambers and placed in wells with culture medium (DMEM). The WS was applied on enamel for 60 min (2 x 30 min) during 1 or 5 days. The extracts (DMEM + WS components that diffused across enamel and dentin) were applied to the cells for 1 h. In the positive control, the bleaching gel with 35% HP was applied for 45 min (3 x 15 min). In the negative control, no treatment was performed. The cell metabolism (MTT Assay) and alkaline phosphatase (ALP) activity were assessed and the data submitted to statistical analysis (One-way Anova/ Tukey’s test; α=5%;). Cell membrane damage was evaluated by flow cytometry. Results: For the MTT and ALP activity assays, there was no significant difference among the bleached groups with WS and negative control, regardless the time of WS application (1 or 5 days) on the cells (p>0.05). However, there was no significant difference among the bleached group with FC for 5 days and positive control. Flow cytometry analysis showed an increase of cell membrane damage only for the positive control. Conclusion: It was concluded that the whitestrip with 10% of HP did not cause significant trans-enamel and trans-dentinal toxic effects to the MDPC-23 cells.