A seed-specific regulator of triterpene saponin biosynthesis in medicago truncatula
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1105/tpc.19.00609 http://hdl.handle.net/11449/200554 |
Resumo: | Plants produce a vast array of defense compounds to protect themselves from pathogen attack or herbivore predation. Saponins are a specific class of defense compounds comprising bioactive glycosides with a steroidal or triterpenoid aglycone backbone. The model legume Medicago truncatula synthesizes two types of saponins, hemolytic saponins and nonhemolytic soyasaponins, which accumulate as specific blends in different plant organs. Here, we report the identification of the seed-specific transcription factor TRITERPENE SAPONIN ACTIVATION REGULATOR3 (TSAR3), which controls hemolytic saponin biosynthesis in developing M. truncatula seeds. Analysis of genes that are coexpressed with TSAR3 in transcriptome data sets from developing M. truncatula seeds led to the identification of CYP88A13, a cytochrome P450 that catalyzes the C-16a hydroxylation of medicagenic acid toward zanhic acid, the final oxidation step of the hemolytic saponin biosynthesis branch in M. truncatula. In addition, two uridine diphosphate glycosyltransferases, UGT73F18 and UGT73F19, which glucosylate hemolytic sapogenins at the C-3 position, were identified. The genes encoding the identified biosynthetic enzymes are present in clusters of duplicated genes in the M. truncatula genome. This appears to be a common theme among saponin biosynthesis genes, especially glycosyltransferases, and may be the driving force of the metabolic evolution of saponins. |
id |
UNSP_cecb5dc79f5e550f544b06b160d78363 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/200554 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
A seed-specific regulator of triterpene saponin biosynthesis in medicago truncatulaPlants produce a vast array of defense compounds to protect themselves from pathogen attack or herbivore predation. Saponins are a specific class of defense compounds comprising bioactive glycosides with a steroidal or triterpenoid aglycone backbone. The model legume Medicago truncatula synthesizes two types of saponins, hemolytic saponins and nonhemolytic soyasaponins, which accumulate as specific blends in different plant organs. Here, we report the identification of the seed-specific transcription factor TRITERPENE SAPONIN ACTIVATION REGULATOR3 (TSAR3), which controls hemolytic saponin biosynthesis in developing M. truncatula seeds. Analysis of genes that are coexpressed with TSAR3 in transcriptome data sets from developing M. truncatula seeds led to the identification of CYP88A13, a cytochrome P450 that catalyzes the C-16a hydroxylation of medicagenic acid toward zanhic acid, the final oxidation step of the hemolytic saponin biosynthesis branch in M. truncatula. In addition, two uridine diphosphate glycosyltransferases, UGT73F18 and UGT73F19, which glucosylate hemolytic sapogenins at the C-3 position, were identified. The genes encoding the identified biosynthetic enzymes are present in clusters of duplicated genes in the M. truncatula genome. This appears to be a common theme among saponin biosynthesis genes, especially glycosyltransferases, and may be the driving force of the metabolic evolution of saponins.Ghent University Department of Plant Biotechnology and BioinformaticsVIB Center for Plant Systems BiologyDepartment of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)Institut de Recherche en Horticulture et Semences-Unités Mixtes de Recherche Université d’Angers INRAE Institut Agro, SFR 4207 QuaSaVVIB Metabolomics CoreDepartment of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)Ghent UniversityVIB Center for Plant Systems BiologyUniversidade Estadual Paulista (Unesp)Institut AgroVIB Metabolomics CoreRibeiro, BiancaLacchini, EliaBicalho, Keylla U. [UNESP]Mertens, JanArendt, PhilippBossche, Robin VandenCalegario, GabrielaGryffroy, LoreCeulemans, EviBuitink, JuliaGoossens, AlainPollier, Jacob2020-12-12T02:09:42Z2020-12-12T02:09:42Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-2042http://dx.doi.org/10.1105/tpc.19.00609Plant Cell, v. 32, n. 6, p. 2020-2042, 2020.1532-298X1040-4651http://hdl.handle.net/11449/20055410.1105/tpc.19.006092-s2.0-85085904658Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlant Cellinfo:eu-repo/semantics/openAccess2021-10-23T14:40:35Zoai:repositorio.unesp.br:11449/200554Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:38:25.305369Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A seed-specific regulator of triterpene saponin biosynthesis in medicago truncatula |
title |
A seed-specific regulator of triterpene saponin biosynthesis in medicago truncatula |
spellingShingle |
A seed-specific regulator of triterpene saponin biosynthesis in medicago truncatula Ribeiro, Bianca |
title_short |
A seed-specific regulator of triterpene saponin biosynthesis in medicago truncatula |
title_full |
A seed-specific regulator of triterpene saponin biosynthesis in medicago truncatula |
title_fullStr |
A seed-specific regulator of triterpene saponin biosynthesis in medicago truncatula |
title_full_unstemmed |
A seed-specific regulator of triterpene saponin biosynthesis in medicago truncatula |
title_sort |
A seed-specific regulator of triterpene saponin biosynthesis in medicago truncatula |
author |
Ribeiro, Bianca |
author_facet |
Ribeiro, Bianca Lacchini, Elia Bicalho, Keylla U. [UNESP] Mertens, Jan Arendt, Philipp Bossche, Robin Vanden Calegario, Gabriela Gryffroy, Lore Ceulemans, Evi Buitink, Julia Goossens, Alain Pollier, Jacob |
author_role |
author |
author2 |
Lacchini, Elia Bicalho, Keylla U. [UNESP] Mertens, Jan Arendt, Philipp Bossche, Robin Vanden Calegario, Gabriela Gryffroy, Lore Ceulemans, Evi Buitink, Julia Goossens, Alain Pollier, Jacob |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Ghent University VIB Center for Plant Systems Biology Universidade Estadual Paulista (Unesp) Institut Agro VIB Metabolomics Core |
dc.contributor.author.fl_str_mv |
Ribeiro, Bianca Lacchini, Elia Bicalho, Keylla U. [UNESP] Mertens, Jan Arendt, Philipp Bossche, Robin Vanden Calegario, Gabriela Gryffroy, Lore Ceulemans, Evi Buitink, Julia Goossens, Alain Pollier, Jacob |
description |
Plants produce a vast array of defense compounds to protect themselves from pathogen attack or herbivore predation. Saponins are a specific class of defense compounds comprising bioactive glycosides with a steroidal or triterpenoid aglycone backbone. The model legume Medicago truncatula synthesizes two types of saponins, hemolytic saponins and nonhemolytic soyasaponins, which accumulate as specific blends in different plant organs. Here, we report the identification of the seed-specific transcription factor TRITERPENE SAPONIN ACTIVATION REGULATOR3 (TSAR3), which controls hemolytic saponin biosynthesis in developing M. truncatula seeds. Analysis of genes that are coexpressed with TSAR3 in transcriptome data sets from developing M. truncatula seeds led to the identification of CYP88A13, a cytochrome P450 that catalyzes the C-16a hydroxylation of medicagenic acid toward zanhic acid, the final oxidation step of the hemolytic saponin biosynthesis branch in M. truncatula. In addition, two uridine diphosphate glycosyltransferases, UGT73F18 and UGT73F19, which glucosylate hemolytic sapogenins at the C-3 position, were identified. The genes encoding the identified biosynthetic enzymes are present in clusters of duplicated genes in the M. truncatula genome. This appears to be a common theme among saponin biosynthesis genes, especially glycosyltransferases, and may be the driving force of the metabolic evolution of saponins. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:09:42Z 2020-12-12T02:09:42Z 2020-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1105/tpc.19.00609 Plant Cell, v. 32, n. 6, p. 2020-2042, 2020. 1532-298X 1040-4651 http://hdl.handle.net/11449/200554 10.1105/tpc.19.00609 2-s2.0-85085904658 |
url |
http://dx.doi.org/10.1105/tpc.19.00609 http://hdl.handle.net/11449/200554 |
identifier_str_mv |
Plant Cell, v. 32, n. 6, p. 2020-2042, 2020. 1532-298X 1040-4651 10.1105/tpc.19.00609 2-s2.0-85085904658 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plant Cell |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2020-2042 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128542986207232 |