Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1807-3107BOR-2020.VOL34.0050 http://hdl.handle.net/11449/200645 |
Resumo: | Candida infection is an important cause of morbidity and mortality in immunocompromised patients. The increase in its incidence has been associated with resistance to antimicrobial therapy and biofilm formation. The aim of this study was to evaluate the efficacy of tea tree oil (TTO) and its main component-terpinen-4-ol-against resistant Candida albicans strains (genotypes A and B) identified by molecular typing and against C. albicans ATCC 90028 and SC 5314 reference strains in planktonic and biofilm cultures. The minimum inhibitory concentration, minimum fungicidal concentration, and rate of biofilm development were used to evaluate antifungal activity. Results were obtained from analysis of the biofilm using the cell proliferation assay 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2Htetrazolium-5-carboxanilide (XTT) and confocal laser scanning microscopy (CLSM). Terpinen-4-ol and TTO inhibited C. albicans growth. CLSM confirmed that 17.92 mg/mL of TTO and 8.86 mg/mL of terpinen-4-ol applied for 60 s (rinse simulation) interfered with biofilm formation. Hence, this in vitro s tudy r evealed t hat n atural substances such as TTO and terpinen-4-ol present promising results for the treatment of oral candidiasis. |
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Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicansBiofilmsCandida albicansMycosesPhytotherapyCandida infection is an important cause of morbidity and mortality in immunocompromised patients. The increase in its incidence has been associated with resistance to antimicrobial therapy and biofilm formation. The aim of this study was to evaluate the efficacy of tea tree oil (TTO) and its main component-terpinen-4-ol-against resistant Candida albicans strains (genotypes A and B) identified by molecular typing and against C. albicans ATCC 90028 and SC 5314 reference strains in planktonic and biofilm cultures. The minimum inhibitory concentration, minimum fungicidal concentration, and rate of biofilm development were used to evaluate antifungal activity. Results were obtained from analysis of the biofilm using the cell proliferation assay 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2Htetrazolium-5-carboxanilide (XTT) and confocal laser scanning microscopy (CLSM). Terpinen-4-ol and TTO inhibited C. albicans growth. CLSM confirmed that 17.92 mg/mL of TTO and 8.86 mg/mL of terpinen-4-ol applied for 60 s (rinse simulation) interfered with biofilm formation. Hence, this in vitro s tudy r evealed t hat n atural substances such as TTO and terpinen-4-ol present promising results for the treatment of oral candidiasis.Universidade Estadual Paulista-Unesp School of Dentistry of Araraquara Department of Physiology and PathologyUniversidade Estadual de Campinas-Unicamp School of Dentistry of Piracicaba Department of Physiological SciencesUniversidade Estadual Paulista-Unesp School of Dentistry of Araraquara Department of Physiology and PathologyUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Francisconi, Renata Serignoli [UNESP]Huacho, Patricia Milagros Maquera [UNESP]Tonon, Caroline Coradi [UNESP]Bordini, Ester Alves Ferreira [UNESP]Correia, Marília Ferreira [UNESP]Sardi, Janaína de Cássia OrlandiSpolidorio, Denise Madalena Palomari [UNESP]2020-12-12T02:12:16Z2020-12-12T02:12:16Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/1807-3107BOR-2020.VOL34.0050Brazilian Oral Research, v. 34.1807-31071806-8324http://hdl.handle.net/11449/20064510.1590/1807-3107BOR-2020.VOL34.0050S1806-832420200001002362-s2.0-85086983898S1806-83242020000100236.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Researchinfo:eu-repo/semantics/openAccess2024-01-09T06:27:49Zoai:repositorio.unesp.br:11449/200645Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-09T06:27:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans |
title |
Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans |
spellingShingle |
Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans Francisconi, Renata Serignoli [UNESP] Biofilms Candida albicans Mycoses Phytotherapy |
title_short |
Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans |
title_full |
Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans |
title_fullStr |
Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans |
title_full_unstemmed |
Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans |
title_sort |
Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans |
author |
Francisconi, Renata Serignoli [UNESP] |
author_facet |
Francisconi, Renata Serignoli [UNESP] Huacho, Patricia Milagros Maquera [UNESP] Tonon, Caroline Coradi [UNESP] Bordini, Ester Alves Ferreira [UNESP] Correia, Marília Ferreira [UNESP] Sardi, Janaína de Cássia Orlandi Spolidorio, Denise Madalena Palomari [UNESP] |
author_role |
author |
author2 |
Huacho, Patricia Milagros Maquera [UNESP] Tonon, Caroline Coradi [UNESP] Bordini, Ester Alves Ferreira [UNESP] Correia, Marília Ferreira [UNESP] Sardi, Janaína de Cássia Orlandi Spolidorio, Denise Madalena Palomari [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Francisconi, Renata Serignoli [UNESP] Huacho, Patricia Milagros Maquera [UNESP] Tonon, Caroline Coradi [UNESP] Bordini, Ester Alves Ferreira [UNESP] Correia, Marília Ferreira [UNESP] Sardi, Janaína de Cássia Orlandi Spolidorio, Denise Madalena Palomari [UNESP] |
dc.subject.por.fl_str_mv |
Biofilms Candida albicans Mycoses Phytotherapy |
topic |
Biofilms Candida albicans Mycoses Phytotherapy |
description |
Candida infection is an important cause of morbidity and mortality in immunocompromised patients. The increase in its incidence has been associated with resistance to antimicrobial therapy and biofilm formation. The aim of this study was to evaluate the efficacy of tea tree oil (TTO) and its main component-terpinen-4-ol-against resistant Candida albicans strains (genotypes A and B) identified by molecular typing and against C. albicans ATCC 90028 and SC 5314 reference strains in planktonic and biofilm cultures. The minimum inhibitory concentration, minimum fungicidal concentration, and rate of biofilm development were used to evaluate antifungal activity. Results were obtained from analysis of the biofilm using the cell proliferation assay 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2Htetrazolium-5-carboxanilide (XTT) and confocal laser scanning microscopy (CLSM). Terpinen-4-ol and TTO inhibited C. albicans growth. CLSM confirmed that 17.92 mg/mL of TTO and 8.86 mg/mL of terpinen-4-ol applied for 60 s (rinse simulation) interfered with biofilm formation. Hence, this in vitro s tudy r evealed t hat n atural substances such as TTO and terpinen-4-ol present promising results for the treatment of oral candidiasis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:12:16Z 2020-12-12T02:12:16Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1807-3107BOR-2020.VOL34.0050 Brazilian Oral Research, v. 34. 1807-3107 1806-8324 http://hdl.handle.net/11449/200645 10.1590/1807-3107BOR-2020.VOL34.0050 S1806-83242020000100236 2-s2.0-85086983898 S1806-83242020000100236.pdf |
url |
http://dx.doi.org/10.1590/1807-3107BOR-2020.VOL34.0050 http://hdl.handle.net/11449/200645 |
identifier_str_mv |
Brazilian Oral Research, v. 34. 1807-3107 1806-8324 10.1590/1807-3107BOR-2020.VOL34.0050 S1806-83242020000100236 2-s2.0-85086983898 S1806-83242020000100236.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Oral Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965567823642624 |