Variant antigen diversity in Trypanosoma vivax is not driven by recombination
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41467-020-14575-8 http://hdl.handle.net/11449/201553 |
Resumo: | African trypanosomes (Trypanosoma) are vector-borne haemoparasites that survive in the vertebrate bloodstream through antigenic variation of their Variant Surface Glycoprotein (VSG). Recombination, or rather segmented gene conversion, is fundamental in Trypanosoma brucei for both VSG gene switching and for generating antigenic diversity during infections. Trypanosoma vivax is a related, livestock pathogen whose VSG lack structures that facilitate gene conversion in T. brucei and mechanisms underlying its antigenic diversity are poorly understood. Here we show that species-wide VSG repertoire is broadly conserved across diverse T. vivax clinical strains and has limited antigenic repertoire. We use variant antigen profiling, coalescent approaches and experimental infections to show that recombination plays little role in diversifying T. vivax VSG sequences. These results have immediate consequences for both the current mechanistic model of antigenic variation in African trypanosomes and species differences in virulence and transmission, requiring reconsideration of the wider epidemiology of animal African trypanosomiasis. |
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Variant antigen diversity in Trypanosoma vivax is not driven by recombinationAfrican trypanosomes (Trypanosoma) are vector-borne haemoparasites that survive in the vertebrate bloodstream through antigenic variation of their Variant Surface Glycoprotein (VSG). Recombination, or rather segmented gene conversion, is fundamental in Trypanosoma brucei for both VSG gene switching and for generating antigenic diversity during infections. Trypanosoma vivax is a related, livestock pathogen whose VSG lack structures that facilitate gene conversion in T. brucei and mechanisms underlying its antigenic diversity are poorly understood. Here we show that species-wide VSG repertoire is broadly conserved across diverse T. vivax clinical strains and has limited antigenic repertoire. We use variant antigen profiling, coalescent approaches and experimental infections to show that recombination plays little role in diversifying T. vivax VSG sequences. These results have immediate consequences for both the current mechanistic model of antigenic variation in African trypanosomes and species differences in virulence and transmission, requiring reconsideration of the wider epidemiology of animal African trypanosomiasis.Biotechnology and Biological Sciences Research CouncilBill and Melinda Gates FoundationWellcome TrustDepartment of Infection Biology Institute of Infection and Global Health University of Liverpool, 146 Brownlow HillDepartment of Veterinary Pathology Faculty of Agrarian and Veterinary Sciences São Paulo State University (UNESP)Institute of Integrative Biology University of Liverpool, Biosciences Building, Crown StreetLivestock Genetic Programme International Livestock Research Institute, 30709 Naivasha RoadInternational Research Centre for Livestock Development in the Sub-humid Zone (CIRDES), No. 559, rue 5-31 angle, Avenue du Gouverneur LouveauDepartment of Parasitology Institute of Biomedical Sciences University of Sao Paulo, Avenue Professor Lineu Prestes, 1374 Cidade UniversitariaDepartment of Veterinary Pathology Faculty of Agrarian and Veterinary Sciences São Paulo State University (UNESP)Biotechnology and Biological Sciences Research Council: BB/M022811/1Biotechnology and Biological Sciences Research Council: BB/R021139/1Bill and Melinda Gates Foundation: GCE Round 11Wellcome Trust: WT206815/Z/17/ZUniversity of LiverpoolUniversidade Estadual Paulista (Unesp)International Livestock Research InstituteInternational Research Centre for Livestock Development in the Sub-humid Zone (CIRDES)Universidade de São Paulo (USP)Silva Pereira, Sarade Almeida Castilho Neto, Kayo J. G. [UNESP]Duffy, Craig W.Richards, PeterNoyes, HarryOgugo, MosesRogério André, Marcos [UNESP]Bengaly, ZakariaKemp, SteveTeixeira, Marta M. G.Machado, Rosangela Z. [UNESP]Jackson, Andrew P.2020-12-12T02:35:34Z2020-12-12T02:35:34Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41467-020-14575-8Nature Communications, v. 11, n. 1, 2020.2041-1723http://hdl.handle.net/11449/20155310.1038/s41467-020-14575-82-s2.0-85079336540Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNature Communicationsinfo:eu-repo/semantics/openAccess2021-10-22T20:18:54Zoai:repositorio.unesp.br:11449/201553Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T20:18:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Variant antigen diversity in Trypanosoma vivax is not driven by recombination |
title |
Variant antigen diversity in Trypanosoma vivax is not driven by recombination |
spellingShingle |
Variant antigen diversity in Trypanosoma vivax is not driven by recombination Silva Pereira, Sara |
title_short |
Variant antigen diversity in Trypanosoma vivax is not driven by recombination |
title_full |
Variant antigen diversity in Trypanosoma vivax is not driven by recombination |
title_fullStr |
Variant antigen diversity in Trypanosoma vivax is not driven by recombination |
title_full_unstemmed |
Variant antigen diversity in Trypanosoma vivax is not driven by recombination |
title_sort |
Variant antigen diversity in Trypanosoma vivax is not driven by recombination |
author |
Silva Pereira, Sara |
author_facet |
Silva Pereira, Sara de Almeida Castilho Neto, Kayo J. G. [UNESP] Duffy, Craig W. Richards, Peter Noyes, Harry Ogugo, Moses Rogério André, Marcos [UNESP] Bengaly, Zakaria Kemp, Steve Teixeira, Marta M. G. Machado, Rosangela Z. [UNESP] Jackson, Andrew P. |
author_role |
author |
author2 |
de Almeida Castilho Neto, Kayo J. G. [UNESP] Duffy, Craig W. Richards, Peter Noyes, Harry Ogugo, Moses Rogério André, Marcos [UNESP] Bengaly, Zakaria Kemp, Steve Teixeira, Marta M. G. Machado, Rosangela Z. [UNESP] Jackson, Andrew P. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Liverpool Universidade Estadual Paulista (Unesp) International Livestock Research Institute International Research Centre for Livestock Development in the Sub-humid Zone (CIRDES) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Silva Pereira, Sara de Almeida Castilho Neto, Kayo J. G. [UNESP] Duffy, Craig W. Richards, Peter Noyes, Harry Ogugo, Moses Rogério André, Marcos [UNESP] Bengaly, Zakaria Kemp, Steve Teixeira, Marta M. G. Machado, Rosangela Z. [UNESP] Jackson, Andrew P. |
description |
African trypanosomes (Trypanosoma) are vector-borne haemoparasites that survive in the vertebrate bloodstream through antigenic variation of their Variant Surface Glycoprotein (VSG). Recombination, or rather segmented gene conversion, is fundamental in Trypanosoma brucei for both VSG gene switching and for generating antigenic diversity during infections. Trypanosoma vivax is a related, livestock pathogen whose VSG lack structures that facilitate gene conversion in T. brucei and mechanisms underlying its antigenic diversity are poorly understood. Here we show that species-wide VSG repertoire is broadly conserved across diverse T. vivax clinical strains and has limited antigenic repertoire. We use variant antigen profiling, coalescent approaches and experimental infections to show that recombination plays little role in diversifying T. vivax VSG sequences. These results have immediate consequences for both the current mechanistic model of antigenic variation in African trypanosomes and species differences in virulence and transmission, requiring reconsideration of the wider epidemiology of animal African trypanosomiasis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:35:34Z 2020-12-12T02:35:34Z 2020-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41467-020-14575-8 Nature Communications, v. 11, n. 1, 2020. 2041-1723 http://hdl.handle.net/11449/201553 10.1038/s41467-020-14575-8 2-s2.0-85079336540 |
url |
http://dx.doi.org/10.1038/s41467-020-14575-8 http://hdl.handle.net/11449/201553 |
identifier_str_mv |
Nature Communications, v. 11, n. 1, 2020. 2041-1723 10.1038/s41467-020-14575-8 2-s2.0-85079336540 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nature Communications |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965344341688320 |