Calcium pretreatment enhances fluoride reactivity with enamel and dentine

Detalhes bibliográficos
Autor(a) principal: Spinola, Manuela Da Silva
Data de Publicação: 2022
Outros Autores: Tenuta, Livia Maria Andalo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2021.105338
http://hdl.handle.net/11449/237555
Resumo: Objective: High fluoride concentration treatments are known to react with enamel and dentine forming calcium fluoride (CaF2)-like deposits, but strategies to improve this reactivity beyond increasing fluoride concentration/ reducing pH in fluoride treatments have not been explored. Here we investigated the ability of a calcium pretreatment to improve fluoride reactivity.& nbsp;Design: In a blind and randomized in vitro study, sound and carious enamel and dentine slabs (n = 11/group) were randomly allocated into one of the following treatments: Deionized water (negative control); 0.05% sodium fluoride (F, positive control); 150 mM calcium lactate solution followed by 0.05% sodium fluoride solution (Ca-*F); 150 mM calcium lactate solution premixed with 0.05% sodium fluoride solution (CaF2, active control). Alkali-soluble fluoride (representing CaF2-like deposits formed on the substrates) was extracted from the slabs using 1 M KOH for 24 h and measured by an ion-specific electrode. Carious slabs were further observed under scanning electron microscopy (SEM). Data were analyzed by two-way ANOVA followed by Tukey test.& nbsp;Results: The Ca-*F treatment enhanced fluoride reactivity with all tested substrates when compared with F alone. Carious substrates had a greater reactivity with F and Ca-*F than their respective sound substrates, confirming that increased porosity enhances the reactivity with fluoride. Alkali-soluble fluoride concentration after the CaF(2 & nbsp;)treatment did not differ among the different substrates, suggesting this treatment causes only contamination with preformed CaF2, which was noted under SEM.& nbsp;Conclusion: A calcium pretreatment enhances the reactivity of fluoride with enamel and dentine.
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spelling Calcium pretreatment enhances fluoride reactivity with enamel and dentineFluorideCalcium fluorideDental cariesRootPreventionObjective: High fluoride concentration treatments are known to react with enamel and dentine forming calcium fluoride (CaF2)-like deposits, but strategies to improve this reactivity beyond increasing fluoride concentration/ reducing pH in fluoride treatments have not been explored. Here we investigated the ability of a calcium pretreatment to improve fluoride reactivity.& nbsp;Design: In a blind and randomized in vitro study, sound and carious enamel and dentine slabs (n = 11/group) were randomly allocated into one of the following treatments: Deionized water (negative control); 0.05% sodium fluoride (F, positive control); 150 mM calcium lactate solution followed by 0.05% sodium fluoride solution (Ca-*F); 150 mM calcium lactate solution premixed with 0.05% sodium fluoride solution (CaF2, active control). Alkali-soluble fluoride (representing CaF2-like deposits formed on the substrates) was extracted from the slabs using 1 M KOH for 24 h and measured by an ion-specific electrode. Carious slabs were further observed under scanning electron microscopy (SEM). Data were analyzed by two-way ANOVA followed by Tukey test.& nbsp;Results: The Ca-*F treatment enhanced fluoride reactivity with all tested substrates when compared with F alone. Carious substrates had a greater reactivity with F and Ca-*F than their respective sound substrates, confirming that increased porosity enhances the reactivity with fluoride. Alkali-soluble fluoride concentration after the CaF(2 & nbsp;)treatment did not differ among the different substrates, suggesting this treatment causes only contamination with preformed CaF2, which was noted under SEM.& nbsp;Conclusion: A calcium pretreatment enhances the reactivity of fluoride with enamel and dentine.University of Michigan College of Engineering and National Science Foundation (NSF, USA)Univ Michigan Sch Dent, Dept Cariol, Restorat Sci & Endodont, 1011 N Univ Ave, Ann Arbor, MI 48109 USAUniv Michigan, Dept Cariol Restorat Sci & Endodont, Sch Dent, 1011 N Univ Ave rm 2217, Ann Arbor, MI 48109 USAUniversity of Michigan College of Engineering and National Science Foundation (NSF, USA): DMR-1625671Elsevier B.V.Univ Michigan Sch DentUniv MichiganUniversidade Estadual Paulista (UNESP)Spinola, Manuela Da SilvaTenuta, Livia Maria Andalo2022-11-30T13:38:29Z2022-11-30T13:38:29Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5http://dx.doi.org/10.1016/j.archoralbio.2021.105338Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 134, 5 p., 2022.0003-9969http://hdl.handle.net/11449/23755510.1016/j.archoralbio.2021.105338WOS:000791259900003Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives Of Oral Biologyinfo:eu-repo/semantics/openAccess2022-11-30T13:38:29Zoai:repositorio.unesp.br:11449/237555Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:53:45.448961Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Calcium pretreatment enhances fluoride reactivity with enamel and dentine
title Calcium pretreatment enhances fluoride reactivity with enamel and dentine
spellingShingle Calcium pretreatment enhances fluoride reactivity with enamel and dentine
Spinola, Manuela Da Silva
Fluoride
Calcium fluoride
Dental caries
Root
Prevention
title_short Calcium pretreatment enhances fluoride reactivity with enamel and dentine
title_full Calcium pretreatment enhances fluoride reactivity with enamel and dentine
title_fullStr Calcium pretreatment enhances fluoride reactivity with enamel and dentine
title_full_unstemmed Calcium pretreatment enhances fluoride reactivity with enamel and dentine
title_sort Calcium pretreatment enhances fluoride reactivity with enamel and dentine
author Spinola, Manuela Da Silva
author_facet Spinola, Manuela Da Silva
Tenuta, Livia Maria Andalo
author_role author
author2 Tenuta, Livia Maria Andalo
author2_role author
dc.contributor.none.fl_str_mv Univ Michigan Sch Dent
Univ Michigan
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Spinola, Manuela Da Silva
Tenuta, Livia Maria Andalo
dc.subject.por.fl_str_mv Fluoride
Calcium fluoride
Dental caries
Root
Prevention
topic Fluoride
Calcium fluoride
Dental caries
Root
Prevention
description Objective: High fluoride concentration treatments are known to react with enamel and dentine forming calcium fluoride (CaF2)-like deposits, but strategies to improve this reactivity beyond increasing fluoride concentration/ reducing pH in fluoride treatments have not been explored. Here we investigated the ability of a calcium pretreatment to improve fluoride reactivity.& nbsp;Design: In a blind and randomized in vitro study, sound and carious enamel and dentine slabs (n = 11/group) were randomly allocated into one of the following treatments: Deionized water (negative control); 0.05% sodium fluoride (F, positive control); 150 mM calcium lactate solution followed by 0.05% sodium fluoride solution (Ca-*F); 150 mM calcium lactate solution premixed with 0.05% sodium fluoride solution (CaF2, active control). Alkali-soluble fluoride (representing CaF2-like deposits formed on the substrates) was extracted from the slabs using 1 M KOH for 24 h and measured by an ion-specific electrode. Carious slabs were further observed under scanning electron microscopy (SEM). Data were analyzed by two-way ANOVA followed by Tukey test.& nbsp;Results: The Ca-*F treatment enhanced fluoride reactivity with all tested substrates when compared with F alone. Carious substrates had a greater reactivity with F and Ca-*F than their respective sound substrates, confirming that increased porosity enhances the reactivity with fluoride. Alkali-soluble fluoride concentration after the CaF(2 & nbsp;)treatment did not differ among the different substrates, suggesting this treatment causes only contamination with preformed CaF2, which was noted under SEM.& nbsp;Conclusion: A calcium pretreatment enhances the reactivity of fluoride with enamel and dentine.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-30T13:38:29Z
2022-11-30T13:38:29Z
2022-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2021.105338
Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 134, 5 p., 2022.
0003-9969
http://hdl.handle.net/11449/237555
10.1016/j.archoralbio.2021.105338
WOS:000791259900003
url http://dx.doi.org/10.1016/j.archoralbio.2021.105338
http://hdl.handle.net/11449/237555
identifier_str_mv Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 134, 5 p., 2022.
0003-9969
10.1016/j.archoralbio.2021.105338
WOS:000791259900003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives Of Oral Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 5
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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