Fast and reliable detection of SARS-CoV-2 antibodies based on surface plasmon resonance
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1039/d1ay00737h http://hdl.handle.net/11449/229286 |
Resumo: | Researchers worldwide have been studying alternatives to detect SARS-CoV-2 (COVID-19), and accurate and timely diagnosis is crucial for controlling the outbreaks of the disease. Surface plasmon resonance (SPR) is an effective strategy based on antibodies, and it can be used for simple and fast detection of antibodies due to COVID-19 infection. Accordingly, this paper reports on the highly sensitive and specific detection of antibody responses to SARS-CoV-2 spike (S) and nucleocapsid (N) proteins in COVID-19 patients. In this methodology, spike (S) and nucleocapsid (N) proteins belonging to the coronavirus genome were immobilized on the surface of a gold sensor using self-assembled monolayers. Previously, serum from COVID-19 patients was screened by immunochromatography-based COVID-19 IgG rapid test and/or ELISA in house to determine the presence of IgG titers. Serum from COVID-19-positive patients presenting with IgG were added on the surface and, at the time they bound to proteins, they caused refractive changes in the SPR angle. The antibody detection limit was determined through successive injections into the SPR apparatus-these injections ranged from pure (without dilution) to 1 : 200 μL. The system has shown good reproducibility between runs after coated surface regeneration with 0.1 M glycine-HCl solution (pH 3.0); all experiments were tested in triplicate. The antibodies targeted both S and N fragments and gave a high assay sensitivity by identifying 19 out of 20 COVID-19-positive patients. Most importantly, the assay time took less than 10 min. The results of this study indicate that the proposed simple strategy demonstrates high sensitivity and time-saving in the detection of SARS-CoV-2 response antibodies. |
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Fast and reliable detection of SARS-CoV-2 antibodies based on surface plasmon resonanceResearchers worldwide have been studying alternatives to detect SARS-CoV-2 (COVID-19), and accurate and timely diagnosis is crucial for controlling the outbreaks of the disease. Surface plasmon resonance (SPR) is an effective strategy based on antibodies, and it can be used for simple and fast detection of antibodies due to COVID-19 infection. Accordingly, this paper reports on the highly sensitive and specific detection of antibody responses to SARS-CoV-2 spike (S) and nucleocapsid (N) proteins in COVID-19 patients. In this methodology, spike (S) and nucleocapsid (N) proteins belonging to the coronavirus genome were immobilized on the surface of a gold sensor using self-assembled monolayers. Previously, serum from COVID-19 patients was screened by immunochromatography-based COVID-19 IgG rapid test and/or ELISA in house to determine the presence of IgG titers. Serum from COVID-19-positive patients presenting with IgG were added on the surface and, at the time they bound to proteins, they caused refractive changes in the SPR angle. The antibody detection limit was determined through successive injections into the SPR apparatus-these injections ranged from pure (without dilution) to 1 : 200 μL. The system has shown good reproducibility between runs after coated surface regeneration with 0.1 M glycine-HCl solution (pH 3.0); all experiments were tested in triplicate. The antibodies targeted both S and N fragments and gave a high assay sensitivity by identifying 19 out of 20 COVID-19-positive patients. Most importantly, the assay time took less than 10 min. The results of this study indicate that the proposed simple strategy demonstrates high sensitivity and time-saving in the detection of SARS-CoV-2 response antibodies.Institute of Biotechnology UNESPDepartment of Chemistry and Biochemistry Institute of Bioscience UNESPDepartment of Infectious Diseases Dermatology Diagnostic Imaging and Radiotherapy Faculty of Medicine of Botucatu UNESPDepartment of Medical Clinic Faculty of Medicine of Botucatu UNESPInstitute of Biotechnology UNESPDepartment of Chemistry and Biochemistry Institute of Bioscience UNESPDepartment of Infectious Diseases Dermatology Diagnostic Imaging and Radiotherapy Faculty of Medicine of Botucatu UNESPDepartment of Medical Clinic Faculty of Medicine of Botucatu UNESPUniversidade Estadual Paulista (UNESP)Basso, Caroline Rodrigues [UNESP]Malossi, Camila Dantas [UNESP]Haisi, Amanda [UNESP]De Albuquerque Pedrosa, Valber [UNESP]Barbosa, Alexandre Naime [UNESP]Grotto, Rejane Tommasini [UNESP]Araujo Junior, João Pessoa [UNESP]2022-04-29T08:31:33Z2022-04-29T08:31:33Z2021-08-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3297-3306http://dx.doi.org/10.1039/d1ay00737hAnalytical Methods, v. 13, n. 29, p. 3297-3306, 2021.1759-96791759-9660http://hdl.handle.net/11449/22928610.1039/d1ay00737h2-s2.0-85111993300Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnalytical Methodsinfo:eu-repo/semantics/openAccess2024-08-15T15:23:01Zoai:repositorio.unesp.br:11449/229286Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:23:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fast and reliable detection of SARS-CoV-2 antibodies based on surface plasmon resonance |
title |
Fast and reliable detection of SARS-CoV-2 antibodies based on surface plasmon resonance |
spellingShingle |
Fast and reliable detection of SARS-CoV-2 antibodies based on surface plasmon resonance Basso, Caroline Rodrigues [UNESP] |
title_short |
Fast and reliable detection of SARS-CoV-2 antibodies based on surface plasmon resonance |
title_full |
Fast and reliable detection of SARS-CoV-2 antibodies based on surface plasmon resonance |
title_fullStr |
Fast and reliable detection of SARS-CoV-2 antibodies based on surface plasmon resonance |
title_full_unstemmed |
Fast and reliable detection of SARS-CoV-2 antibodies based on surface plasmon resonance |
title_sort |
Fast and reliable detection of SARS-CoV-2 antibodies based on surface plasmon resonance |
author |
Basso, Caroline Rodrigues [UNESP] |
author_facet |
Basso, Caroline Rodrigues [UNESP] Malossi, Camila Dantas [UNESP] Haisi, Amanda [UNESP] De Albuquerque Pedrosa, Valber [UNESP] Barbosa, Alexandre Naime [UNESP] Grotto, Rejane Tommasini [UNESP] Araujo Junior, João Pessoa [UNESP] |
author_role |
author |
author2 |
Malossi, Camila Dantas [UNESP] Haisi, Amanda [UNESP] De Albuquerque Pedrosa, Valber [UNESP] Barbosa, Alexandre Naime [UNESP] Grotto, Rejane Tommasini [UNESP] Araujo Junior, João Pessoa [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Basso, Caroline Rodrigues [UNESP] Malossi, Camila Dantas [UNESP] Haisi, Amanda [UNESP] De Albuquerque Pedrosa, Valber [UNESP] Barbosa, Alexandre Naime [UNESP] Grotto, Rejane Tommasini [UNESP] Araujo Junior, João Pessoa [UNESP] |
description |
Researchers worldwide have been studying alternatives to detect SARS-CoV-2 (COVID-19), and accurate and timely diagnosis is crucial for controlling the outbreaks of the disease. Surface plasmon resonance (SPR) is an effective strategy based on antibodies, and it can be used for simple and fast detection of antibodies due to COVID-19 infection. Accordingly, this paper reports on the highly sensitive and specific detection of antibody responses to SARS-CoV-2 spike (S) and nucleocapsid (N) proteins in COVID-19 patients. In this methodology, spike (S) and nucleocapsid (N) proteins belonging to the coronavirus genome were immobilized on the surface of a gold sensor using self-assembled monolayers. Previously, serum from COVID-19 patients was screened by immunochromatography-based COVID-19 IgG rapid test and/or ELISA in house to determine the presence of IgG titers. Serum from COVID-19-positive patients presenting with IgG were added on the surface and, at the time they bound to proteins, they caused refractive changes in the SPR angle. The antibody detection limit was determined through successive injections into the SPR apparatus-these injections ranged from pure (without dilution) to 1 : 200 μL. The system has shown good reproducibility between runs after coated surface regeneration with 0.1 M glycine-HCl solution (pH 3.0); all experiments were tested in triplicate. The antibodies targeted both S and N fragments and gave a high assay sensitivity by identifying 19 out of 20 COVID-19-positive patients. Most importantly, the assay time took less than 10 min. The results of this study indicate that the proposed simple strategy demonstrates high sensitivity and time-saving in the detection of SARS-CoV-2 response antibodies. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-07 2022-04-29T08:31:33Z 2022-04-29T08:31:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1039/d1ay00737h Analytical Methods, v. 13, n. 29, p. 3297-3306, 2021. 1759-9679 1759-9660 http://hdl.handle.net/11449/229286 10.1039/d1ay00737h 2-s2.0-85111993300 |
url |
http://dx.doi.org/10.1039/d1ay00737h http://hdl.handle.net/11449/229286 |
identifier_str_mv |
Analytical Methods, v. 13, n. 29, p. 3297-3306, 2021. 1759-9679 1759-9660 10.1039/d1ay00737h 2-s2.0-85111993300 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Analytical Methods |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3297-3306 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128162120335360 |