Synchronization of Leishmania amazonensis Cell Cycle Using Hydroxyurea
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Capítulo de livro |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/978-1-0716-2736-5_10 http://hdl.handle.net/11449/240740 |
Resumo: | Leishmania spp. comprises a group of protozoan parasites that affect millions of people around the world. Understanding the main cell cycle-dependent events could provide an important route for developing specific therapies since some factors involved in cell cycle control may have low similarity relative to their homologs in mammals. Furthermore, accurate cell cycle-dependent analyses often require many cells, which can be achieved through cell cycle synchronization. Here, we described a useful method to synchronize procyclic promastigote forms of Leishmania amazonensis using hydroxyurea (HU) and the analysis of its DNA content profile. This approach can be extended to other trypanosomatids, such as Trypanosoma cruzi or Trypanosoma brucei, and provides an effective method for arresting more than 80% of cells at the G1/S phase transition. |
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Repositório Institucional da UNESP |
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Synchronization of Leishmania amazonensis Cell Cycle Using HydroxyureaCell cycle synchronizationDNA content profileHydroxyureaLeishmania amazonensisLeishmania spp. comprises a group of protozoan parasites that affect millions of people around the world. Understanding the main cell cycle-dependent events could provide an important route for developing specific therapies since some factors involved in cell cycle control may have low similarity relative to their homologs in mammals. Furthermore, accurate cell cycle-dependent analyses often require many cells, which can be achieved through cell cycle synchronization. Here, we described a useful method to synchronize procyclic promastigote forms of Leishmania amazonensis using hydroxyurea (HU) and the analysis of its DNA content profile. This approach can be extended to other trypanosomatids, such as Trypanosoma cruzi or Trypanosoma brucei, and provides an effective method for arresting more than 80% of cells at the G1/S phase transition.Telomeres Laboratory Department of Chemical and Biological Sciences Biosciences Institute São Paulo State University (UNESP), SPDNA Replication and Repair Laboratory (DRRL) Department of Chemical and Biological Sciences Biosciences Institute São Paulo State University (UNESP), SPTelomeres Laboratory Department of Chemical and Biological Sciences Biosciences Institute São Paulo State University (UNESP), SPDNA Replication and Repair Laboratory (DRRL) Department of Chemical and Biological Sciences Biosciences Institute São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)Oliveira, Beatriz C. D. de [UNESP]Assis, Luiz H. C. [UNESP]Shiburah, Mark E. [UNESP]Paiva, Stephany C. [UNESP]Fontes, Veronica S. [UNESP]Oliveira, Leilane S. de [UNESP]Silva, Vitor L. da [UNESP]Silva, Marcelo S. da [UNESP]Cano, Maria Isabel N. [UNESP]2023-03-01T20:30:49Z2023-03-01T20:30:49Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPart127-135http://dx.doi.org/10.1007/978-1-0716-2736-5_10Methods in Molecular Biology, v. 2579, p. 127-135.1940-60291064-3745http://hdl.handle.net/11449/24074010.1007/978-1-0716-2736-5_102-s2.0-85137031152Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMethods in Molecular Biologyinfo:eu-repo/semantics/openAccess2023-03-01T20:30:49Zoai:repositorio.unesp.br:11449/240740Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:53:53.301148Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Synchronization of Leishmania amazonensis Cell Cycle Using Hydroxyurea |
title |
Synchronization of Leishmania amazonensis Cell Cycle Using Hydroxyurea |
spellingShingle |
Synchronization of Leishmania amazonensis Cell Cycle Using Hydroxyurea Oliveira, Beatriz C. D. de [UNESP] Cell cycle synchronization DNA content profile Hydroxyurea Leishmania amazonensis |
title_short |
Synchronization of Leishmania amazonensis Cell Cycle Using Hydroxyurea |
title_full |
Synchronization of Leishmania amazonensis Cell Cycle Using Hydroxyurea |
title_fullStr |
Synchronization of Leishmania amazonensis Cell Cycle Using Hydroxyurea |
title_full_unstemmed |
Synchronization of Leishmania amazonensis Cell Cycle Using Hydroxyurea |
title_sort |
Synchronization of Leishmania amazonensis Cell Cycle Using Hydroxyurea |
author |
Oliveira, Beatriz C. D. de [UNESP] |
author_facet |
Oliveira, Beatriz C. D. de [UNESP] Assis, Luiz H. C. [UNESP] Shiburah, Mark E. [UNESP] Paiva, Stephany C. [UNESP] Fontes, Veronica S. [UNESP] Oliveira, Leilane S. de [UNESP] Silva, Vitor L. da [UNESP] Silva, Marcelo S. da [UNESP] Cano, Maria Isabel N. [UNESP] |
author_role |
author |
author2 |
Assis, Luiz H. C. [UNESP] Shiburah, Mark E. [UNESP] Paiva, Stephany C. [UNESP] Fontes, Veronica S. [UNESP] Oliveira, Leilane S. de [UNESP] Silva, Vitor L. da [UNESP] Silva, Marcelo S. da [UNESP] Cano, Maria Isabel N. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Oliveira, Beatriz C. D. de [UNESP] Assis, Luiz H. C. [UNESP] Shiburah, Mark E. [UNESP] Paiva, Stephany C. [UNESP] Fontes, Veronica S. [UNESP] Oliveira, Leilane S. de [UNESP] Silva, Vitor L. da [UNESP] Silva, Marcelo S. da [UNESP] Cano, Maria Isabel N. [UNESP] |
dc.subject.por.fl_str_mv |
Cell cycle synchronization DNA content profile Hydroxyurea Leishmania amazonensis |
topic |
Cell cycle synchronization DNA content profile Hydroxyurea Leishmania amazonensis |
description |
Leishmania spp. comprises a group of protozoan parasites that affect millions of people around the world. Understanding the main cell cycle-dependent events could provide an important route for developing specific therapies since some factors involved in cell cycle control may have low similarity relative to their homologs in mammals. Furthermore, accurate cell cycle-dependent analyses often require many cells, which can be achieved through cell cycle synchronization. Here, we described a useful method to synchronize procyclic promastigote forms of Leishmania amazonensis using hydroxyurea (HU) and the analysis of its DNA content profile. This approach can be extended to other trypanosomatids, such as Trypanosoma cruzi or Trypanosoma brucei, and provides an effective method for arresting more than 80% of cells at the G1/S phase transition. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-01T20:30:49Z 2023-03-01T20:30:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/bookPart |
format |
bookPart |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/978-1-0716-2736-5_10 Methods in Molecular Biology, v. 2579, p. 127-135. 1940-6029 1064-3745 http://hdl.handle.net/11449/240740 10.1007/978-1-0716-2736-5_10 2-s2.0-85137031152 |
url |
http://dx.doi.org/10.1007/978-1-0716-2736-5_10 http://hdl.handle.net/11449/240740 |
identifier_str_mv |
Methods in Molecular Biology, v. 2579, p. 127-135. 1940-6029 1064-3745 10.1007/978-1-0716-2736-5_10 2-s2.0-85137031152 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Methods in Molecular Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
127-135 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128718517829632 |