The chemical composition and pharmacological activities of geopropolis produced by Melipona fasciculata Smith in northeast Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5455/jmp.20150204115607 http://hdl.handle.net/11449/137218 |
Resumo: | Aim: Geopropolis is produced by stingless bees from resinous materials of plants, adding salivary secretions, wax, mud or clay, and has been used in folk medicine for the treatment of respiratory diseases and dermatoses. Therefore, it is important to study its antibacterial, antitumor and immunomodulatory properties are important to confirm the ethnopharmacological applications. This work aimed to evaluate its chemical composition, and its antimicrobial, anti-tumoral and immunomodulatory activities. Materials and Methods: Geopropolis composition was investigated using GC-MS analysis. Antibacterial tests were performed to determine the minimum inhibitory concentration. HEp-2 cells viability was determined by the reduction of MTT, and cytokine production by human monocytes was determined by ELISA. Results: The major constituents of geopropolis compounds were carbohydrates and their derivatives, triterpenes, anacardic acid, alkylresorcinols, and sugar alcohols. Geopropolis alone showed no antibacterial activity against Staphylococcus aureus and Escherichia coli, but its combination with chloramphenicol exerted a greater action against S. aureus than chloramphenicol alone. Geopropolis exhibited a cytostatic action toward human laryngeal epidermoid carcinoma cells and stimulated tumor necrosis factor alpha and interleukin-10 production by human monocytes, showing an activator profile for human monocytes. Conclusion: The synergistic effect of geopropolis and chloramphenicol deserves further investigation due to bacterial resistance to antibiotics. Geopropolis also displayed antitumoral and immunomodulatory activity, and its biological properties may be due to triterpenes - one of its major chemical constituents. |
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The chemical composition and pharmacological activities of geopropolis produced by Melipona fasciculata Smith in northeast BrazilChemical compositionCytokineGeopropolisHuman laryngeal epidermoid-2 cellsPharmacological activitiesAim: Geopropolis is produced by stingless bees from resinous materials of plants, adding salivary secretions, wax, mud or clay, and has been used in folk medicine for the treatment of respiratory diseases and dermatoses. Therefore, it is important to study its antibacterial, antitumor and immunomodulatory properties are important to confirm the ethnopharmacological applications. This work aimed to evaluate its chemical composition, and its antimicrobial, anti-tumoral and immunomodulatory activities. Materials and Methods: Geopropolis composition was investigated using GC-MS analysis. Antibacterial tests were performed to determine the minimum inhibitory concentration. HEp-2 cells viability was determined by the reduction of MTT, and cytokine production by human monocytes was determined by ELISA. Results: The major constituents of geopropolis compounds were carbohydrates and their derivatives, triterpenes, anacardic acid, alkylresorcinols, and sugar alcohols. Geopropolis alone showed no antibacterial activity against Staphylococcus aureus and Escherichia coli, but its combination with chloramphenicol exerted a greater action against S. aureus than chloramphenicol alone. Geopropolis exhibited a cytostatic action toward human laryngeal epidermoid carcinoma cells and stimulated tumor necrosis factor alpha and interleukin-10 production by human monocytes, showing an activator profile for human monocytes. Conclusion: The synergistic effect of geopropolis and chloramphenicol deserves further investigation due to bacterial resistance to antibiotics. Geopropolis also displayed antitumoral and immunomodulatory activity, and its biological properties may be due to triterpenes - one of its major chemical constituents.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Instituto de Biociências de Botucatu (IBB), Departamento de Microbiologia e Imunologia, Botucatu, SP, BrasilBulgarian Academy of Sciences, Institute of Organic Chemistry with Centre of Phytochemistry, Sofia, BulgariaUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Instituto de Biociências de Botucatu (IBB), Departamento de Microbiologia e Imunologia, Botucatu, SP, BrasilFAPESP: 2010/50064-7FAPESP: 2010/01028-8Universidade Estadual Paulista (Unesp)Bulgarian Academy of SciencesAraújo, Maria José Abigail Mendes [UNESP]Búfalo, Michelle Cristiane [UNESP]Conti, Bruno José [UNESP]Fernandes Jr., Ary [UNESP]Trusheva, BoryanaBankova, VassyaSforcin, José Maurício [UNESP]2016-04-01T18:44:44Z2016-04-01T18:44:44Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12-20application/pdfhttp://dx.doi.org/10.5455/jmp.20150204115607Journal of Molecular Pathophysiology, v. 4, n. 1, p. 12-20, 2015.2146-832Xhttp://hdl.handle.net/11449/13721810.5455/jmp.20150204115607ISSN2146-832X-2015-04-01-12-20.pdfCurrículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Molecular Pathophysiologyinfo:eu-repo/semantics/openAccess2023-12-26T06:14:13Zoai:repositorio.unesp.br:11449/137218Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-26T06:14:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The chemical composition and pharmacological activities of geopropolis produced by Melipona fasciculata Smith in northeast Brazil |
title |
The chemical composition and pharmacological activities of geopropolis produced by Melipona fasciculata Smith in northeast Brazil |
spellingShingle |
The chemical composition and pharmacological activities of geopropolis produced by Melipona fasciculata Smith in northeast Brazil Araújo, Maria José Abigail Mendes [UNESP] Chemical composition Cytokine Geopropolis Human laryngeal epidermoid-2 cells Pharmacological activities |
title_short |
The chemical composition and pharmacological activities of geopropolis produced by Melipona fasciculata Smith in northeast Brazil |
title_full |
The chemical composition and pharmacological activities of geopropolis produced by Melipona fasciculata Smith in northeast Brazil |
title_fullStr |
The chemical composition and pharmacological activities of geopropolis produced by Melipona fasciculata Smith in northeast Brazil |
title_full_unstemmed |
The chemical composition and pharmacological activities of geopropolis produced by Melipona fasciculata Smith in northeast Brazil |
title_sort |
The chemical composition and pharmacological activities of geopropolis produced by Melipona fasciculata Smith in northeast Brazil |
author |
Araújo, Maria José Abigail Mendes [UNESP] |
author_facet |
Araújo, Maria José Abigail Mendes [UNESP] Búfalo, Michelle Cristiane [UNESP] Conti, Bruno José [UNESP] Fernandes Jr., Ary [UNESP] Trusheva, Boryana Bankova, Vassya Sforcin, José Maurício [UNESP] |
author_role |
author |
author2 |
Búfalo, Michelle Cristiane [UNESP] Conti, Bruno José [UNESP] Fernandes Jr., Ary [UNESP] Trusheva, Boryana Bankova, Vassya Sforcin, José Maurício [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Bulgarian Academy of Sciences |
dc.contributor.author.fl_str_mv |
Araújo, Maria José Abigail Mendes [UNESP] Búfalo, Michelle Cristiane [UNESP] Conti, Bruno José [UNESP] Fernandes Jr., Ary [UNESP] Trusheva, Boryana Bankova, Vassya Sforcin, José Maurício [UNESP] |
dc.subject.por.fl_str_mv |
Chemical composition Cytokine Geopropolis Human laryngeal epidermoid-2 cells Pharmacological activities |
topic |
Chemical composition Cytokine Geopropolis Human laryngeal epidermoid-2 cells Pharmacological activities |
description |
Aim: Geopropolis is produced by stingless bees from resinous materials of plants, adding salivary secretions, wax, mud or clay, and has been used in folk medicine for the treatment of respiratory diseases and dermatoses. Therefore, it is important to study its antibacterial, antitumor and immunomodulatory properties are important to confirm the ethnopharmacological applications. This work aimed to evaluate its chemical composition, and its antimicrobial, anti-tumoral and immunomodulatory activities. Materials and Methods: Geopropolis composition was investigated using GC-MS analysis. Antibacterial tests were performed to determine the minimum inhibitory concentration. HEp-2 cells viability was determined by the reduction of MTT, and cytokine production by human monocytes was determined by ELISA. Results: The major constituents of geopropolis compounds were carbohydrates and their derivatives, triterpenes, anacardic acid, alkylresorcinols, and sugar alcohols. Geopropolis alone showed no antibacterial activity against Staphylococcus aureus and Escherichia coli, but its combination with chloramphenicol exerted a greater action against S. aureus than chloramphenicol alone. Geopropolis exhibited a cytostatic action toward human laryngeal epidermoid carcinoma cells and stimulated tumor necrosis factor alpha and interleukin-10 production by human monocytes, showing an activator profile for human monocytes. Conclusion: The synergistic effect of geopropolis and chloramphenicol deserves further investigation due to bacterial resistance to antibiotics. Geopropolis also displayed antitumoral and immunomodulatory activity, and its biological properties may be due to triterpenes - one of its major chemical constituents. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2016-04-01T18:44:44Z 2016-04-01T18:44:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5455/jmp.20150204115607 Journal of Molecular Pathophysiology, v. 4, n. 1, p. 12-20, 2015. 2146-832X http://hdl.handle.net/11449/137218 10.5455/jmp.20150204115607 ISSN2146-832X-2015-04-01-12-20.pdf |
url |
http://dx.doi.org/10.5455/jmp.20150204115607 http://hdl.handle.net/11449/137218 |
identifier_str_mv |
Journal of Molecular Pathophysiology, v. 4, n. 1, p. 12-20, 2015. 2146-832X 10.5455/jmp.20150204115607 ISSN2146-832X-2015-04-01-12-20.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Molecular Pathophysiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12-20 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965407915802624 |