Mitotic Chromosome Preparations of Freshwater Stingrays

Detalhes bibliográficos
Autor(a) principal: Paes da Cruz, V. [UNESP]
Data de Publicação: 2015
Outros Autores: Foresti, F. [UNESP]
Tipo de documento: Capítulo de livro
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1201/b18534-8
http://hdl.handle.net/11449/247596
Resumo: The technique for obtaining mitotic chromosomes proposed by Foresti et al. (1981) when applied in freshwater stingrays didn’t result in good quality chromosome preparations. Only a few studies have used this technique to obtain good quality chromosomes in this fi sh group (Valentim et al. 2006; Cruz et al. 2011). Therefore, it was necessary to adapt the technique to freshwater stingrays. Classical in vivo methods basically consist of mitotic stimulation followed by colchicine injection, according to the technique described in Chapter 2. In this chapter we present modifi cations of this technique to obtain a better quality of chromosomes in stingrays. Typically, the kidney tissue is used in this technique, but in stingrays the tissue that showed the best quality and quantity of metaphases was the spleen. Other important modifi cations are the time of colchicine and hypotonic treatments.
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spelling Mitotic Chromosome Preparations of Freshwater StingraysThe technique for obtaining mitotic chromosomes proposed by Foresti et al. (1981) when applied in freshwater stingrays didn’t result in good quality chromosome preparations. Only a few studies have used this technique to obtain good quality chromosomes in this fi sh group (Valentim et al. 2006; Cruz et al. 2011). Therefore, it was necessary to adapt the technique to freshwater stingrays. Classical in vivo methods basically consist of mitotic stimulation followed by colchicine injection, according to the technique described in Chapter 2. In this chapter we present modifi cations of this technique to obtain a better quality of chromosomes in stingrays. Typically, the kidney tissue is used in this technique, but in stingrays the tissue that showed the best quality and quantity of metaphases was the spleen. Other important modifi cations are the time of colchicine and hypotonic treatments.Departamento de Morfologia Instituto de Biociências de Botucatu UNESP, SPDepartamento de Morfologia Instituto de Biociências de Botucatu UNESP, SPUniversidade Estadual Paulista (UNESP)Paes da Cruz, V. [UNESP]Foresti, F. [UNESP]2023-07-29T13:20:21Z2023-07-29T13:20:21Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPart32-36http://dx.doi.org/10.1201/b18534-8Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans, p. 32-36.http://hdl.handle.net/11449/24759610.1201/b18534-82-s2.0-85136235156Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyansinfo:eu-repo/semantics/openAccess2023-07-29T13:20:21Zoai:repositorio.unesp.br:11449/247596Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:42:48.528528Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mitotic Chromosome Preparations of Freshwater Stingrays
title Mitotic Chromosome Preparations of Freshwater Stingrays
spellingShingle Mitotic Chromosome Preparations of Freshwater Stingrays
Paes da Cruz, V. [UNESP]
title_short Mitotic Chromosome Preparations of Freshwater Stingrays
title_full Mitotic Chromosome Preparations of Freshwater Stingrays
title_fullStr Mitotic Chromosome Preparations of Freshwater Stingrays
title_full_unstemmed Mitotic Chromosome Preparations of Freshwater Stingrays
title_sort Mitotic Chromosome Preparations of Freshwater Stingrays
author Paes da Cruz, V. [UNESP]
author_facet Paes da Cruz, V. [UNESP]
Foresti, F. [UNESP]
author_role author
author2 Foresti, F. [UNESP]
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Paes da Cruz, V. [UNESP]
Foresti, F. [UNESP]
description The technique for obtaining mitotic chromosomes proposed by Foresti et al. (1981) when applied in freshwater stingrays didn’t result in good quality chromosome preparations. Only a few studies have used this technique to obtain good quality chromosomes in this fi sh group (Valentim et al. 2006; Cruz et al. 2011). Therefore, it was necessary to adapt the technique to freshwater stingrays. Classical in vivo methods basically consist of mitotic stimulation followed by colchicine injection, according to the technique described in Chapter 2. In this chapter we present modifi cations of this technique to obtain a better quality of chromosomes in stingrays. Typically, the kidney tissue is used in this technique, but in stingrays the tissue that showed the best quality and quantity of metaphases was the spleen. Other important modifi cations are the time of colchicine and hypotonic treatments.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01
2023-07-29T13:20:21Z
2023-07-29T13:20:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1201/b18534-8
Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans, p. 32-36.
http://hdl.handle.net/11449/247596
10.1201/b18534-8
2-s2.0-85136235156
url http://dx.doi.org/10.1201/b18534-8
http://hdl.handle.net/11449/247596
identifier_str_mv Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans, p. 32-36.
10.1201/b18534-8
2-s2.0-85136235156
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 32-36
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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