Mitotic Chromosome Preparations of Freshwater Stingrays
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | |
Tipo de documento: | Capítulo de livro |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1201/b18534-8 http://hdl.handle.net/11449/247596 |
Resumo: | The technique for obtaining mitotic chromosomes proposed by Foresti et al. (1981) when applied in freshwater stingrays didn’t result in good quality chromosome preparations. Only a few studies have used this technique to obtain good quality chromosomes in this fi sh group (Valentim et al. 2006; Cruz et al. 2011). Therefore, it was necessary to adapt the technique to freshwater stingrays. Classical in vivo methods basically consist of mitotic stimulation followed by colchicine injection, according to the technique described in Chapter 2. In this chapter we present modifi cations of this technique to obtain a better quality of chromosomes in stingrays. Typically, the kidney tissue is used in this technique, but in stingrays the tissue that showed the best quality and quantity of metaphases was the spleen. Other important modifi cations are the time of colchicine and hypotonic treatments. |
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Mitotic Chromosome Preparations of Freshwater StingraysThe technique for obtaining mitotic chromosomes proposed by Foresti et al. (1981) when applied in freshwater stingrays didn’t result in good quality chromosome preparations. Only a few studies have used this technique to obtain good quality chromosomes in this fi sh group (Valentim et al. 2006; Cruz et al. 2011). Therefore, it was necessary to adapt the technique to freshwater stingrays. Classical in vivo methods basically consist of mitotic stimulation followed by colchicine injection, according to the technique described in Chapter 2. In this chapter we present modifi cations of this technique to obtain a better quality of chromosomes in stingrays. Typically, the kidney tissue is used in this technique, but in stingrays the tissue that showed the best quality and quantity of metaphases was the spleen. Other important modifi cations are the time of colchicine and hypotonic treatments.Departamento de Morfologia Instituto de Biociências de Botucatu UNESP, SPDepartamento de Morfologia Instituto de Biociências de Botucatu UNESP, SPUniversidade Estadual Paulista (UNESP)Paes da Cruz, V. [UNESP]Foresti, F. [UNESP]2023-07-29T13:20:21Z2023-07-29T13:20:21Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPart32-36http://dx.doi.org/10.1201/b18534-8Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans, p. 32-36.http://hdl.handle.net/11449/24759610.1201/b18534-82-s2.0-85136235156Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyansinfo:eu-repo/semantics/openAccess2023-07-29T13:20:21Zoai:repositorio.unesp.br:11449/247596Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:42:48.528528Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mitotic Chromosome Preparations of Freshwater Stingrays |
title |
Mitotic Chromosome Preparations of Freshwater Stingrays |
spellingShingle |
Mitotic Chromosome Preparations of Freshwater Stingrays Paes da Cruz, V. [UNESP] |
title_short |
Mitotic Chromosome Preparations of Freshwater Stingrays |
title_full |
Mitotic Chromosome Preparations of Freshwater Stingrays |
title_fullStr |
Mitotic Chromosome Preparations of Freshwater Stingrays |
title_full_unstemmed |
Mitotic Chromosome Preparations of Freshwater Stingrays |
title_sort |
Mitotic Chromosome Preparations of Freshwater Stingrays |
author |
Paes da Cruz, V. [UNESP] |
author_facet |
Paes da Cruz, V. [UNESP] Foresti, F. [UNESP] |
author_role |
author |
author2 |
Foresti, F. [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Paes da Cruz, V. [UNESP] Foresti, F. [UNESP] |
description |
The technique for obtaining mitotic chromosomes proposed by Foresti et al. (1981) when applied in freshwater stingrays didn’t result in good quality chromosome preparations. Only a few studies have used this technique to obtain good quality chromosomes in this fi sh group (Valentim et al. 2006; Cruz et al. 2011). Therefore, it was necessary to adapt the technique to freshwater stingrays. Classical in vivo methods basically consist of mitotic stimulation followed by colchicine injection, according to the technique described in Chapter 2. In this chapter we present modifi cations of this technique to obtain a better quality of chromosomes in stingrays. Typically, the kidney tissue is used in this technique, but in stingrays the tissue that showed the best quality and quantity of metaphases was the spleen. Other important modifi cations are the time of colchicine and hypotonic treatments. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2023-07-29T13:20:21Z 2023-07-29T13:20:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/bookPart |
format |
bookPart |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1201/b18534-8 Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans, p. 32-36. http://hdl.handle.net/11449/247596 10.1201/b18534-8 2-s2.0-85136235156 |
url |
http://dx.doi.org/10.1201/b18534-8 http://hdl.handle.net/11449/247596 |
identifier_str_mv |
Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans, p. 32-36. 10.1201/b18534-8 2-s2.0-85136235156 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
32-36 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129545119727616 |