Lectinhistochemical staining of granuloma induced by bacillus Calmette- Guerin in Piaractus mesopotamicus
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0122-02682013000300002 http://hdl.handle.net/11449/113210 |
Resumo: | Objetive: This study was conducted to evaluate, by means of lectinhistochemistry (LHC), the expression of carbohydrates in granulomas induced by the bacillus Calmette-Guerin (BCG) in muscle tissue of Piaractus mesopotamicus after 33 days. Material and methods. Histological sections with 3 mu m thick were incubated with the following lectins : WGA (Wheat germ agglutinin), DBA (Dolichos biflorus agglutinin) and HPA (Helix pomatia agglutinin), and the results were evaluated by light microscopy. Results. Acid fast bacilli were stained by Ziehl Neelsen (ZN) and strong labeled by WGA in the cytoplasm of macrophages. Labeling with DBA was intense in fibroblasts and weak in macrophages. On the other hand, HPA binding was stronger in macrophages, especially in those that were in close contact with epithelioid cells, without evidence of binding to fibroblasts. The epithelioid cells were not labeled by the used lectins, but they were identified by Hematoxilin-Eosin (HE). The lectins labeled specific type saccharides in glycoproteins, as N-acetylglucosamine present in bacilli and macrophages, as well as N-acetyl-galactosamine in macrophages. The control group showed no inflammation or lectin binding. Conclusions. This technique may be useful in identifying receptors for WGA, DBA and the HPA lectins in epithelioid granuloma induced by BCG in P. mesopotamicus. |
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Lectinhistochemical staining of granuloma induced by bacillus Calmette- Guerin in Piaractus mesopotamicusGlycansgranulomalectinhistochemistrymycobacteriumTeleostObjetive: This study was conducted to evaluate, by means of lectinhistochemistry (LHC), the expression of carbohydrates in granulomas induced by the bacillus Calmette-Guerin (BCG) in muscle tissue of Piaractus mesopotamicus after 33 days. Material and methods. Histological sections with 3 mu m thick were incubated with the following lectins : WGA (Wheat germ agglutinin), DBA (Dolichos biflorus agglutinin) and HPA (Helix pomatia agglutinin), and the results were evaluated by light microscopy. Results. Acid fast bacilli were stained by Ziehl Neelsen (ZN) and strong labeled by WGA in the cytoplasm of macrophages. Labeling with DBA was intense in fibroblasts and weak in macrophages. On the other hand, HPA binding was stronger in macrophages, especially in those that were in close contact with epithelioid cells, without evidence of binding to fibroblasts. The epithelioid cells were not labeled by the used lectins, but they were identified by Hematoxilin-Eosin (HE). The lectins labeled specific type saccharides in glycoproteins, as N-acetylglucosamine present in bacilli and macrophages, as well as N-acetyl-galactosamine in macrophages. The control group showed no inflammation or lectin binding. Conclusions. This technique may be useful in identifying receptors for WGA, DBA and the HPA lectins in epithelioid granuloma induced by BCG in P. mesopotamicus.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Sch Agr & Vet Sci, Dept Vet Pathol, BR-14884900 Jaboticabal, SP, BrazilNatl Univ La Plata, Dept Anim Pathol, Fac Vet Med, La Plata, Buenos Aires, ArgentinaSao Paulo State Univ, Sch Agr & Vet Sci, Dept Vet Pathol, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 09/17640-7Univ CordobaUniversidade Estadual Paulista (Unesp)Natl Univ La PlataManrique, Wilson G. [UNESP]Claudiano, Gustavo S. [UNESP]Figueiredo, Mayra A. P. [UNESP]Petrillo, Thalita R. [UNESP]Marcusso, Paulo F. [UNESP]Gimeno, Eduardo J.Moraes, Julieta R. E. [UNESP]Moraes, Flavio R. [UNESP]2014-12-03T13:11:30Z2014-12-03T13:11:30Z2013-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3753-3758application/pdfhttp://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0122-02682013000300002Revista Mvz Cordoba. Monteria: Univ Cordoba, v. 18, n. 3, p. 3753-3758, 2013.0122-0268http://hdl.handle.net/11449/113210S0122-02682013000300002WOS:000330510900002S0122-02682013000300002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista MVZ Cordoba0.1620,173info:eu-repo/semantics/openAccess2023-11-10T06:09:19Zoai:repositorio.unesp.br:11449/113210Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-10T06:09:19Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Lectinhistochemical staining of granuloma induced by bacillus Calmette- Guerin in Piaractus mesopotamicus |
title |
Lectinhistochemical staining of granuloma induced by bacillus Calmette- Guerin in Piaractus mesopotamicus |
spellingShingle |
Lectinhistochemical staining of granuloma induced by bacillus Calmette- Guerin in Piaractus mesopotamicus Manrique, Wilson G. [UNESP] Glycans granuloma lectinhistochemistry mycobacterium Teleost |
title_short |
Lectinhistochemical staining of granuloma induced by bacillus Calmette- Guerin in Piaractus mesopotamicus |
title_full |
Lectinhistochemical staining of granuloma induced by bacillus Calmette- Guerin in Piaractus mesopotamicus |
title_fullStr |
Lectinhistochemical staining of granuloma induced by bacillus Calmette- Guerin in Piaractus mesopotamicus |
title_full_unstemmed |
Lectinhistochemical staining of granuloma induced by bacillus Calmette- Guerin in Piaractus mesopotamicus |
title_sort |
Lectinhistochemical staining of granuloma induced by bacillus Calmette- Guerin in Piaractus mesopotamicus |
author |
Manrique, Wilson G. [UNESP] |
author_facet |
Manrique, Wilson G. [UNESP] Claudiano, Gustavo S. [UNESP] Figueiredo, Mayra A. P. [UNESP] Petrillo, Thalita R. [UNESP] Marcusso, Paulo F. [UNESP] Gimeno, Eduardo J. Moraes, Julieta R. E. [UNESP] Moraes, Flavio R. [UNESP] |
author_role |
author |
author2 |
Claudiano, Gustavo S. [UNESP] Figueiredo, Mayra A. P. [UNESP] Petrillo, Thalita R. [UNESP] Marcusso, Paulo F. [UNESP] Gimeno, Eduardo J. Moraes, Julieta R. E. [UNESP] Moraes, Flavio R. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Natl Univ La Plata |
dc.contributor.author.fl_str_mv |
Manrique, Wilson G. [UNESP] Claudiano, Gustavo S. [UNESP] Figueiredo, Mayra A. P. [UNESP] Petrillo, Thalita R. [UNESP] Marcusso, Paulo F. [UNESP] Gimeno, Eduardo J. Moraes, Julieta R. E. [UNESP] Moraes, Flavio R. [UNESP] |
dc.subject.por.fl_str_mv |
Glycans granuloma lectinhistochemistry mycobacterium Teleost |
topic |
Glycans granuloma lectinhistochemistry mycobacterium Teleost |
description |
Objetive: This study was conducted to evaluate, by means of lectinhistochemistry (LHC), the expression of carbohydrates in granulomas induced by the bacillus Calmette-Guerin (BCG) in muscle tissue of Piaractus mesopotamicus after 33 days. Material and methods. Histological sections with 3 mu m thick were incubated with the following lectins : WGA (Wheat germ agglutinin), DBA (Dolichos biflorus agglutinin) and HPA (Helix pomatia agglutinin), and the results were evaluated by light microscopy. Results. Acid fast bacilli were stained by Ziehl Neelsen (ZN) and strong labeled by WGA in the cytoplasm of macrophages. Labeling with DBA was intense in fibroblasts and weak in macrophages. On the other hand, HPA binding was stronger in macrophages, especially in those that were in close contact with epithelioid cells, without evidence of binding to fibroblasts. The epithelioid cells were not labeled by the used lectins, but they were identified by Hematoxilin-Eosin (HE). The lectins labeled specific type saccharides in glycoproteins, as N-acetylglucosamine present in bacilli and macrophages, as well as N-acetyl-galactosamine in macrophages. The control group showed no inflammation or lectin binding. Conclusions. This technique may be useful in identifying receptors for WGA, DBA and the HPA lectins in epithelioid granuloma induced by BCG in P. mesopotamicus. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-01 2014-12-03T13:11:30Z 2014-12-03T13:11:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0122-02682013000300002 Revista Mvz Cordoba. Monteria: Univ Cordoba, v. 18, n. 3, p. 3753-3758, 2013. 0122-0268 http://hdl.handle.net/11449/113210 S0122-02682013000300002 WOS:000330510900002 S0122-02682013000300002.pdf |
url |
http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0122-02682013000300002 http://hdl.handle.net/11449/113210 |
identifier_str_mv |
Revista Mvz Cordoba. Monteria: Univ Cordoba, v. 18, n. 3, p. 3753-3758, 2013. 0122-0268 S0122-02682013000300002 WOS:000330510900002 S0122-02682013000300002.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista MVZ Cordoba 0.162 0,173 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3753-3758 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Cordoba |
publisher.none.fl_str_mv |
Univ Cordoba |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964880100392960 |