Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[ a] pyrene and cyclophosphamide
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-4685-GMB-2018-0038 http://hdl.handle.net/11449/196334 |
Resumo: | This study evaluated the genotoxicity, mutagenicity, antigenotoxicity, and antimutagenicity effects on biochemical parameters of oxidative stress of the Spondias dulcis bark ethanolic extract on mice. The extract was evaluated in the doses of 500, 1000, and 1500 mg/kg bw via gavage. To evaluate the protective effects of the extract, benzo[a] pyrene (B[a]P) and cyclophosphamide (CP) were chosen as DNA damage inducers. Genotoxicity and antigenotoxicity were evaluated by the comet assay. Cytotoxicity, mutagenicity, and antimutagenicity were evaluated by the micronucleus test in bone marrow and peripheral blood. The biochemical parameters of oxidative stress were evaluated by the quantification of catalase activity (CAT) and reduced glutathione (GSH) in total blood, liver and kidney, and malondialdehyde (MDA), in liver and kidney. No genotoxic, cytotoxic, or mutagenic effect was found on mice exposed to the extract. The extract depleted the number of damaged nucleoids in total blood and the number of micronucleus (MN) in both cell types. The extract was able to increase CAT activity and GSH levels and decrease MDA levels after treatment with B[a]P and CP. The results indicate that the S. dulcis extract has potential to be used as preventive compound against DNA damage caused by CP and B[a]P. |
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Repositório Institucional da UNESP |
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Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[ a] pyrene and cyclophosphamideComet assaymicronucleus testSpondias dulciscytotoxicitymutagenicityThis study evaluated the genotoxicity, mutagenicity, antigenotoxicity, and antimutagenicity effects on biochemical parameters of oxidative stress of the Spondias dulcis bark ethanolic extract on mice. The extract was evaluated in the doses of 500, 1000, and 1500 mg/kg bw via gavage. To evaluate the protective effects of the extract, benzo[a] pyrene (B[a]P) and cyclophosphamide (CP) were chosen as DNA damage inducers. Genotoxicity and antigenotoxicity were evaluated by the comet assay. Cytotoxicity, mutagenicity, and antimutagenicity were evaluated by the micronucleus test in bone marrow and peripheral blood. The biochemical parameters of oxidative stress were evaluated by the quantification of catalase activity (CAT) and reduced glutathione (GSH) in total blood, liver and kidney, and malondialdehyde (MDA), in liver and kidney. No genotoxic, cytotoxic, or mutagenic effect was found on mice exposed to the extract. The extract depleted the number of damaged nucleoids in total blood and the number of micronucleus (MN) in both cell types. The extract was able to increase CAT activity and GSH levels and decrease MDA levels after treatment with B[a]P and CP. The results indicate that the S. dulcis extract has potential to be used as preventive compound against DNA damage caused by CP and B[a]P.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Julio de Mesquita FilhoUniversidade do Oeste Paulista (UNOESTE)Univ Oeste Paulista, Fac Artes Ciencias Letras & Educ, Presidente Prudente, SP, BrazilUniv Oeste Paulista, Fac Med, 700 R Jose Bongiovani, BR-19050920 Presidente Prudente, SP, BrazilUniv Oeste Paulista, Fac Farm, Presidente Prudente, SP, BrazilUniv Estadual Paulista, Dept Fis Quim & Biol, Presidente Prudente, SP, BrazilAPTA, Presidente Prudente, SP, BrazilUniv Estadual Paulista, Dept Fis Quim & Biol, Presidente Prudente, SP, BrazilCAPES: 001Soc Brasil GeneticaUniv Oeste PaulistaUniversidade Estadual Paulista (Unesp)APTAAraujo, Caroline de S.Brito, Lorrane D.Tarifa, Marina O.Farah da Silva, Nayara J.Rodrigues, Karoline S.Cavalcante, Dalita G. S. M. [UNESP]Gomes, Andressa S. [UNESP]Zocoler, Marcos A.Yoshihara, EidiCamparoto, Marjori L.Job, Aldo E. [UNESP]Kerche, Leandra E.2020-12-10T19:41:20Z2020-12-10T19:41:20Z2019-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article643-654application/pdfhttp://dx.doi.org/10.1590/1678-4685-GMB-2018-0038Genetics And Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 42, n. 3, p. 643-654, 2019.1415-4757http://hdl.handle.net/11449/19633410.1590/1678-4685-GMB-2018-0038S1415-47572019000400643WOS:000496934400016S1415-47572019000400643.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics And Molecular Biologyinfo:eu-repo/semantics/openAccess2024-06-19T12:44:50Zoai:repositorio.unesp.br:11449/196334Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-19T12:44:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[ a] pyrene and cyclophosphamide |
title |
Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[ a] pyrene and cyclophosphamide |
spellingShingle |
Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[ a] pyrene and cyclophosphamide Araujo, Caroline de S. Comet assay micronucleus test Spondias dulcis cytotoxicity mutagenicity |
title_short |
Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[ a] pyrene and cyclophosphamide |
title_full |
Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[ a] pyrene and cyclophosphamide |
title_fullStr |
Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[ a] pyrene and cyclophosphamide |
title_full_unstemmed |
Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[ a] pyrene and cyclophosphamide |
title_sort |
Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[ a] pyrene and cyclophosphamide |
author |
Araujo, Caroline de S. |
author_facet |
Araujo, Caroline de S. Brito, Lorrane D. Tarifa, Marina O. Farah da Silva, Nayara J. Rodrigues, Karoline S. Cavalcante, Dalita G. S. M. [UNESP] Gomes, Andressa S. [UNESP] Zocoler, Marcos A. Yoshihara, Eidi Camparoto, Marjori L. Job, Aldo E. [UNESP] Kerche, Leandra E. |
author_role |
author |
author2 |
Brito, Lorrane D. Tarifa, Marina O. Farah da Silva, Nayara J. Rodrigues, Karoline S. Cavalcante, Dalita G. S. M. [UNESP] Gomes, Andressa S. [UNESP] Zocoler, Marcos A. Yoshihara, Eidi Camparoto, Marjori L. Job, Aldo E. [UNESP] Kerche, Leandra E. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Oeste Paulista Universidade Estadual Paulista (Unesp) APTA |
dc.contributor.author.fl_str_mv |
Araujo, Caroline de S. Brito, Lorrane D. Tarifa, Marina O. Farah da Silva, Nayara J. Rodrigues, Karoline S. Cavalcante, Dalita G. S. M. [UNESP] Gomes, Andressa S. [UNESP] Zocoler, Marcos A. Yoshihara, Eidi Camparoto, Marjori L. Job, Aldo E. [UNESP] Kerche, Leandra E. |
dc.subject.por.fl_str_mv |
Comet assay micronucleus test Spondias dulcis cytotoxicity mutagenicity |
topic |
Comet assay micronucleus test Spondias dulcis cytotoxicity mutagenicity |
description |
This study evaluated the genotoxicity, mutagenicity, antigenotoxicity, and antimutagenicity effects on biochemical parameters of oxidative stress of the Spondias dulcis bark ethanolic extract on mice. The extract was evaluated in the doses of 500, 1000, and 1500 mg/kg bw via gavage. To evaluate the protective effects of the extract, benzo[a] pyrene (B[a]P) and cyclophosphamide (CP) were chosen as DNA damage inducers. Genotoxicity and antigenotoxicity were evaluated by the comet assay. Cytotoxicity, mutagenicity, and antimutagenicity were evaluated by the micronucleus test in bone marrow and peripheral blood. The biochemical parameters of oxidative stress were evaluated by the quantification of catalase activity (CAT) and reduced glutathione (GSH) in total blood, liver and kidney, and malondialdehyde (MDA), in liver and kidney. No genotoxic, cytotoxic, or mutagenic effect was found on mice exposed to the extract. The extract depleted the number of damaged nucleoids in total blood and the number of micronucleus (MN) in both cell types. The extract was able to increase CAT activity and GSH levels and decrease MDA levels after treatment with B[a]P and CP. The results indicate that the S. dulcis extract has potential to be used as preventive compound against DNA damage caused by CP and B[a]P. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-01 2020-12-10T19:41:20Z 2020-12-10T19:41:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4685-GMB-2018-0038 Genetics And Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 42, n. 3, p. 643-654, 2019. 1415-4757 http://hdl.handle.net/11449/196334 10.1590/1678-4685-GMB-2018-0038 S1415-47572019000400643 WOS:000496934400016 S1415-47572019000400643.pdf |
url |
http://dx.doi.org/10.1590/1678-4685-GMB-2018-0038 http://hdl.handle.net/11449/196334 |
identifier_str_mv |
Genetics And Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 42, n. 3, p. 643-654, 2019. 1415-4757 10.1590/1678-4685-GMB-2018-0038 S1415-47572019000400643 WOS:000496934400016 S1415-47572019000400643.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics And Molecular Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
643-654 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasil Genetica |
publisher.none.fl_str_mv |
Soc Brasil Genetica |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650142104977408 |