Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae)
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572008000300025 http://hdl.handle.net/11449/10797 |
Resumo: | The northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) is very rich in anthocyanins, natural pigments which have strong antioxidant properties and potential health benefits, resulting in the worldwide use the blueberry as a medicinal plant. We investigated the mutagenic potential of simple hydroalcoholic extracts of V. corymbosum acutely administrated by gavage to Swiss mice at doses of 1 g kg-1, 1.5 g kg-1 and 2 g kg-1. Peripheral blood cells were collected 4 h and 24 h post-gavage and assessed by the alkaline comet assay, with further blood samples being collected at 48 h and 72 h for assessment using the micronucleus (MN) assay. Our results show that the V. corymbosum extracts did not induce any statistically significant increase in the average amount of DNA damage in peripheral blood leukocytes. However, we did record a significant increase in the frequency of micronucleated polychromatic erythrocytes at the three doses tested. |
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Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae)comet assaymicronucleusmutagenicityVaccinium corymbosumThe northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) is very rich in anthocyanins, natural pigments which have strong antioxidant properties and potential health benefits, resulting in the worldwide use the blueberry as a medicinal plant. We investigated the mutagenic potential of simple hydroalcoholic extracts of V. corymbosum acutely administrated by gavage to Swiss mice at doses of 1 g kg-1, 1.5 g kg-1 and 2 g kg-1. Peripheral blood cells were collected 4 h and 24 h post-gavage and assessed by the alkaline comet assay, with further blood samples being collected at 48 h and 72 h for assessment using the micronucleus (MN) assay. Our results show that the V. corymbosum extracts did not induce any statistically significant increase in the average amount of DNA damage in peripheral blood leukocytes. However, we did record a significant increase in the frequency of micronucleated polychromatic erythrocytes at the three doses tested.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade José do Rosário Vellano Faculdade de EnfermagemUniversidade do Vale do Itajaí Núcleo de Investigações Químico-FarmacêuticasUniversidade Estadual Paulista Faculdade de Filosofia e Ciências Departamento de FonoaudiologiaUniversidade Estadual Paulista Faculdade de Filosofia e Ciências Departamento de FonoaudiologiaSociedade Brasileira de GenéticaUniversidade José do Rosário Vellano Faculdade de EnfermagemUniversidade do Vale do Itajaí Núcleo de Investigações Químico-FarmacêuticasUniversidade Estadual Paulista (Unesp)Freitas, Patrícia ScotiniAndrade, Sérgio Faloni deMaistro, Edson Luis [UNESP]2014-05-20T13:31:41Z2014-05-20T13:31:41Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article555-560application/pdfhttp://dx.doi.org/10.1590/S1415-47572008000300025Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 2, p. 555-560, 2008.1415-4757http://hdl.handle.net/11449/1079710.1590/S1415-47572008000300025S1415-47572008000300025WOS:000256957500025S1415-47572008000300025.pdf47875216130383150000-0003-0757-7876SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2024-08-09T17:40:17Zoai:repositorio.unesp.br:11449/10797Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-09T17:40:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae) |
title |
Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae) |
spellingShingle |
Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae) Freitas, Patrícia Scotini comet assay micronucleus mutagenicity Vaccinium corymbosum |
title_short |
Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae) |
title_full |
Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae) |
title_fullStr |
Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae) |
title_full_unstemmed |
Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae) |
title_sort |
Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae) |
author |
Freitas, Patrícia Scotini |
author_facet |
Freitas, Patrícia Scotini Andrade, Sérgio Faloni de Maistro, Edson Luis [UNESP] |
author_role |
author |
author2 |
Andrade, Sérgio Faloni de Maistro, Edson Luis [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade José do Rosário Vellano Faculdade de Enfermagem Universidade do Vale do Itajaí Núcleo de Investigações Químico-Farmacêuticas Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Freitas, Patrícia Scotini Andrade, Sérgio Faloni de Maistro, Edson Luis [UNESP] |
dc.subject.por.fl_str_mv |
comet assay micronucleus mutagenicity Vaccinium corymbosum |
topic |
comet assay micronucleus mutagenicity Vaccinium corymbosum |
description |
The northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) is very rich in anthocyanins, natural pigments which have strong antioxidant properties and potential health benefits, resulting in the worldwide use the blueberry as a medicinal plant. We investigated the mutagenic potential of simple hydroalcoholic extracts of V. corymbosum acutely administrated by gavage to Swiss mice at doses of 1 g kg-1, 1.5 g kg-1 and 2 g kg-1. Peripheral blood cells were collected 4 h and 24 h post-gavage and assessed by the alkaline comet assay, with further blood samples being collected at 48 h and 72 h for assessment using the micronucleus (MN) assay. Our results show that the V. corymbosum extracts did not induce any statistically significant increase in the average amount of DNA damage in peripheral blood leukocytes. However, we did record a significant increase in the frequency of micronucleated polychromatic erythrocytes at the three doses tested. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2014-05-20T13:31:41Z 2014-05-20T13:31:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572008000300025 Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 2, p. 555-560, 2008. 1415-4757 http://hdl.handle.net/11449/10797 10.1590/S1415-47572008000300025 S1415-47572008000300025 WOS:000256957500025 S1415-47572008000300025.pdf 4787521613038315 0000-0003-0757-7876 |
url |
http://dx.doi.org/10.1590/S1415-47572008000300025 http://hdl.handle.net/11449/10797 |
identifier_str_mv |
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 2, p. 555-560, 2008. 1415-4757 10.1590/S1415-47572008000300025 S1415-47572008000300025 WOS:000256957500025 S1415-47572008000300025.pdf 4787521613038315 0000-0003-0757-7876 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
555-560 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128205552353280 |