GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season

Detalhes bibliográficos
Autor(a) principal: Brambila-Souza, Gabriela [UNESP]
Data de Publicação: 2021
Outros Autores: Mylonas, Constantinos Chrysanthos, de Mello, Paulo Henrique [UNESP], Kuradomi, Rafael Yutaka, Batlouni, Sergio Ricardo [UNESP], Tolussi, Carlos Eduardo, Moreira, Renata Guimarães
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/are.15435
http://hdl.handle.net/11449/229072
Resumo: We evaluated the effect of gonadotropin in controlled-release implant together with temperature manipulation in the reproduction of Astyanax altiparanae (lambari) during the non-breeding season. Three groups of adult females were maintained at 20°C and three at 28°C. For each temperature, one group was treated with 4 μg GnRHa g−1, other with 8 μg GnRHa g−1 and other was sham implanted as a control during 4 weeks. Afterwards, 4 females/experimental group were induced to spawn with human chorionic gonadotropin, while 4 females/group were injected with saline. GnRHa implants increased fshβ and lhβ gene expression in females maintained at 28°C. The vtgA gene expression increased in the high GnRHa dose at 20°C, while this higher GnRHa dose associated with the higher temperature increased Gonadosomatic Index. The hCG injection increased the plasma Mature Induction Steroid levels and trigger ovulation, except in control at 28°C. The control females at 20°C spawned but with low reproductive performance, confirming that this is not a limiting temperature for reproduction. The administration of GnRHa as implant increased gonadotropin gene expression at 28°C, temperature at which GSI also increased. Qualitatively, the implants improved reproduction at 20°C, providing offspring out of the reproductive season, improving lambari production throughout the year.
id UNSP_d2f22d54e0af42eec40287afe9274bb1
oai_identifier_str oai:repositorio.unesp.br:11449/229072
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive seasongonadotropinsneotropical fishovariessteroidsvitellogeninWe evaluated the effect of gonadotropin in controlled-release implant together with temperature manipulation in the reproduction of Astyanax altiparanae (lambari) during the non-breeding season. Three groups of adult females were maintained at 20°C and three at 28°C. For each temperature, one group was treated with 4 μg GnRHa g−1, other with 8 μg GnRHa g−1 and other was sham implanted as a control during 4 weeks. Afterwards, 4 females/experimental group were induced to spawn with human chorionic gonadotropin, while 4 females/group were injected with saline. GnRHa implants increased fshβ and lhβ gene expression in females maintained at 28°C. The vtgA gene expression increased in the high GnRHa dose at 20°C, while this higher GnRHa dose associated with the higher temperature increased Gonadosomatic Index. The hCG injection increased the plasma Mature Induction Steroid levels and trigger ovulation, except in control at 28°C. The control females at 20°C spawned but with low reproductive performance, confirming that this is not a limiting temperature for reproduction. The administration of GnRHa as implant increased gonadotropin gene expression at 28°C, temperature at which GSI also increased. Qualitatively, the implants improved reproduction at 20°C, providing offspring out of the reproductive season, improving lambari production throughout the year.UNESP- Universidade Estadual Paulista - Centro de Aquicultura (CAUNESP)Institute of Marine Biology Biotechnology and Aquaculture Hellenic Center for Marine ResearchUniversidade Federal do AmazonasUniversidade Anhembi MorumbiInstituto de Biociências –Universidade de São Paulo (USP)UNESP- Universidade Estadual Paulista - Centro de Aquicultura (CAUNESP)Universidade Estadual Paulista (UNESP)Hellenic Center for Marine ResearchUniversidade Federal do AmazonasUniversidade Anhembi MorumbiUniversidade de São Paulo (USP)Brambila-Souza, Gabriela [UNESP]Mylonas, Constantinos Chrysanthosde Mello, Paulo Henrique [UNESP]Kuradomi, Rafael YutakaBatlouni, Sergio Ricardo [UNESP]Tolussi, Carlos EduardoMoreira, Renata Guimarães2022-04-29T08:30:15Z2022-04-29T08:30:15Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/are.15435Aquaculture Research.1365-21091355-557Xhttp://hdl.handle.net/11449/22907210.1111/are.154352-s2.0-85109047323Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquaculture Researchinfo:eu-repo/semantics/openAccess2024-04-09T15:43:25Zoai:repositorio.unesp.br:11449/229072Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:35:45.014495Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season
title GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season
spellingShingle GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season
Brambila-Souza, Gabriela [UNESP]
gonadotropins
neotropical fish
ovaries
steroids
vitellogenin
title_short GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season
title_full GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season
title_fullStr GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season
title_full_unstemmed GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season
title_sort GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season
author Brambila-Souza, Gabriela [UNESP]
author_facet Brambila-Souza, Gabriela [UNESP]
Mylonas, Constantinos Chrysanthos
de Mello, Paulo Henrique [UNESP]
Kuradomi, Rafael Yutaka
Batlouni, Sergio Ricardo [UNESP]
Tolussi, Carlos Eduardo
Moreira, Renata Guimarães
author_role author
author2 Mylonas, Constantinos Chrysanthos
de Mello, Paulo Henrique [UNESP]
Kuradomi, Rafael Yutaka
Batlouni, Sergio Ricardo [UNESP]
Tolussi, Carlos Eduardo
Moreira, Renata Guimarães
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Hellenic Center for Marine Research
Universidade Federal do Amazonas
Universidade Anhembi Morumbi
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Brambila-Souza, Gabriela [UNESP]
Mylonas, Constantinos Chrysanthos
de Mello, Paulo Henrique [UNESP]
Kuradomi, Rafael Yutaka
Batlouni, Sergio Ricardo [UNESP]
Tolussi, Carlos Eduardo
Moreira, Renata Guimarães
dc.subject.por.fl_str_mv gonadotropins
neotropical fish
ovaries
steroids
vitellogenin
topic gonadotropins
neotropical fish
ovaries
steroids
vitellogenin
description We evaluated the effect of gonadotropin in controlled-release implant together with temperature manipulation in the reproduction of Astyanax altiparanae (lambari) during the non-breeding season. Three groups of adult females were maintained at 20°C and three at 28°C. For each temperature, one group was treated with 4 μg GnRHa g−1, other with 8 μg GnRHa g−1 and other was sham implanted as a control during 4 weeks. Afterwards, 4 females/experimental group were induced to spawn with human chorionic gonadotropin, while 4 females/group were injected with saline. GnRHa implants increased fshβ and lhβ gene expression in females maintained at 28°C. The vtgA gene expression increased in the high GnRHa dose at 20°C, while this higher GnRHa dose associated with the higher temperature increased Gonadosomatic Index. The hCG injection increased the plasma Mature Induction Steroid levels and trigger ovulation, except in control at 28°C. The control females at 20°C spawned but with low reproductive performance, confirming that this is not a limiting temperature for reproduction. The administration of GnRHa as implant increased gonadotropin gene expression at 28°C, temperature at which GSI also increased. Qualitatively, the implants improved reproduction at 20°C, providing offspring out of the reproductive season, improving lambari production throughout the year.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
2022-04-29T08:30:15Z
2022-04-29T08:30:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/are.15435
Aquaculture Research.
1365-2109
1355-557X
http://hdl.handle.net/11449/229072
10.1111/are.15435
2-s2.0-85109047323
url http://dx.doi.org/10.1111/are.15435
http://hdl.handle.net/11449/229072
identifier_str_mv Aquaculture Research.
1365-2109
1355-557X
10.1111/are.15435
2-s2.0-85109047323
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Aquaculture Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129441107279872