GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/are.15435 http://hdl.handle.net/11449/229072 |
Resumo: | We evaluated the effect of gonadotropin in controlled-release implant together with temperature manipulation in the reproduction of Astyanax altiparanae (lambari) during the non-breeding season. Three groups of adult females were maintained at 20°C and three at 28°C. For each temperature, one group was treated with 4 μg GnRHa g−1, other with 8 μg GnRHa g−1 and other was sham implanted as a control during 4 weeks. Afterwards, 4 females/experimental group were induced to spawn with human chorionic gonadotropin, while 4 females/group were injected with saline. GnRHa implants increased fshβ and lhβ gene expression in females maintained at 28°C. The vtgA gene expression increased in the high GnRHa dose at 20°C, while this higher GnRHa dose associated with the higher temperature increased Gonadosomatic Index. The hCG injection increased the plasma Mature Induction Steroid levels and trigger ovulation, except in control at 28°C. The control females at 20°C spawned but with low reproductive performance, confirming that this is not a limiting temperature for reproduction. The administration of GnRHa as implant increased gonadotropin gene expression at 28°C, temperature at which GSI also increased. Qualitatively, the implants improved reproduction at 20°C, providing offspring out of the reproductive season, improving lambari production throughout the year. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive seasongonadotropinsneotropical fishovariessteroidsvitellogeninWe evaluated the effect of gonadotropin in controlled-release implant together with temperature manipulation in the reproduction of Astyanax altiparanae (lambari) during the non-breeding season. Three groups of adult females were maintained at 20°C and three at 28°C. For each temperature, one group was treated with 4 μg GnRHa g−1, other with 8 μg GnRHa g−1 and other was sham implanted as a control during 4 weeks. Afterwards, 4 females/experimental group were induced to spawn with human chorionic gonadotropin, while 4 females/group were injected with saline. GnRHa implants increased fshβ and lhβ gene expression in females maintained at 28°C. The vtgA gene expression increased in the high GnRHa dose at 20°C, while this higher GnRHa dose associated with the higher temperature increased Gonadosomatic Index. The hCG injection increased the plasma Mature Induction Steroid levels and trigger ovulation, except in control at 28°C. The control females at 20°C spawned but with low reproductive performance, confirming that this is not a limiting temperature for reproduction. The administration of GnRHa as implant increased gonadotropin gene expression at 28°C, temperature at which GSI also increased. Qualitatively, the implants improved reproduction at 20°C, providing offspring out of the reproductive season, improving lambari production throughout the year.UNESP- Universidade Estadual Paulista - Centro de Aquicultura (CAUNESP)Institute of Marine Biology Biotechnology and Aquaculture Hellenic Center for Marine ResearchUniversidade Federal do AmazonasUniversidade Anhembi MorumbiInstituto de Biociências –Universidade de São Paulo (USP)UNESP- Universidade Estadual Paulista - Centro de Aquicultura (CAUNESP)Universidade Estadual Paulista (UNESP)Hellenic Center for Marine ResearchUniversidade Federal do AmazonasUniversidade Anhembi MorumbiUniversidade de São Paulo (USP)Brambila-Souza, Gabriela [UNESP]Mylonas, Constantinos Chrysanthosde Mello, Paulo Henrique [UNESP]Kuradomi, Rafael YutakaBatlouni, Sergio Ricardo [UNESP]Tolussi, Carlos EduardoMoreira, Renata Guimarães2022-04-29T08:30:15Z2022-04-29T08:30:15Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/are.15435Aquaculture Research.1365-21091355-557Xhttp://hdl.handle.net/11449/22907210.1111/are.154352-s2.0-85109047323Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquaculture Researchinfo:eu-repo/semantics/openAccess2024-04-09T15:43:25Zoai:repositorio.unesp.br:11449/229072Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:35:45.014495Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season |
title |
GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season |
spellingShingle |
GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season Brambila-Souza, Gabriela [UNESP] gonadotropins neotropical fish ovaries steroids vitellogenin |
title_short |
GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season |
title_full |
GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season |
title_fullStr |
GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season |
title_full_unstemmed |
GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season |
title_sort |
GnRHa implants and temperature modulate Lambari-do-rabo-amarelo, Astyanax altiparanae (Characiformes: Characidae) induced reproduction out of the reproductive season |
author |
Brambila-Souza, Gabriela [UNESP] |
author_facet |
Brambila-Souza, Gabriela [UNESP] Mylonas, Constantinos Chrysanthos de Mello, Paulo Henrique [UNESP] Kuradomi, Rafael Yutaka Batlouni, Sergio Ricardo [UNESP] Tolussi, Carlos Eduardo Moreira, Renata Guimarães |
author_role |
author |
author2 |
Mylonas, Constantinos Chrysanthos de Mello, Paulo Henrique [UNESP] Kuradomi, Rafael Yutaka Batlouni, Sergio Ricardo [UNESP] Tolussi, Carlos Eduardo Moreira, Renata Guimarães |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Hellenic Center for Marine Research Universidade Federal do Amazonas Universidade Anhembi Morumbi Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Brambila-Souza, Gabriela [UNESP] Mylonas, Constantinos Chrysanthos de Mello, Paulo Henrique [UNESP] Kuradomi, Rafael Yutaka Batlouni, Sergio Ricardo [UNESP] Tolussi, Carlos Eduardo Moreira, Renata Guimarães |
dc.subject.por.fl_str_mv |
gonadotropins neotropical fish ovaries steroids vitellogenin |
topic |
gonadotropins neotropical fish ovaries steroids vitellogenin |
description |
We evaluated the effect of gonadotropin in controlled-release implant together with temperature manipulation in the reproduction of Astyanax altiparanae (lambari) during the non-breeding season. Three groups of adult females were maintained at 20°C and three at 28°C. For each temperature, one group was treated with 4 μg GnRHa g−1, other with 8 μg GnRHa g−1 and other was sham implanted as a control during 4 weeks. Afterwards, 4 females/experimental group were induced to spawn with human chorionic gonadotropin, while 4 females/group were injected with saline. GnRHa implants increased fshβ and lhβ gene expression in females maintained at 28°C. The vtgA gene expression increased in the high GnRHa dose at 20°C, while this higher GnRHa dose associated with the higher temperature increased Gonadosomatic Index. The hCG injection increased the plasma Mature Induction Steroid levels and trigger ovulation, except in control at 28°C. The control females at 20°C spawned but with low reproductive performance, confirming that this is not a limiting temperature for reproduction. The administration of GnRHa as implant increased gonadotropin gene expression at 28°C, temperature at which GSI also increased. Qualitatively, the implants improved reproduction at 20°C, providing offspring out of the reproductive season, improving lambari production throughout the year. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:30:15Z 2022-04-29T08:30:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/are.15435 Aquaculture Research. 1365-2109 1355-557X http://hdl.handle.net/11449/229072 10.1111/are.15435 2-s2.0-85109047323 |
url |
http://dx.doi.org/10.1111/are.15435 http://hdl.handle.net/11449/229072 |
identifier_str_mv |
Aquaculture Research. 1365-2109 1355-557X 10.1111/are.15435 2-s2.0-85109047323 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129441107279872 |