Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2018.11.013 http://hdl.handle.net/11449/189676 |
Resumo: | Background: Bone marrow mesenchymal stem cells (BM-MSCs) are undifferentiated cells that can proliferate and differentiate into specialized cells for tissue self-repair. Low-level laser (LLL) can induce biomodulatory effects such as cellular proliferation, differentiation, and migration. We investigated the biomodulatory effects of the photoactive compound chloroaluminum phthalocyanine nanoemulsion (AlClPc/NE) on the adipogenic differentiation of BM-MSCs, when combined with LLL (AlClPc/NE-LLL). Methods: The BM-MSCs used in this work were isolated from green fluorescent protein-positive (GFP + ) C57BL6 mice. Cells were first treated with AlClPc/NE, a well-designed photoactive nano-drug and were then subjected to in vitro expansion, morphological and immunophenotypic characterization, and cellular cytotoxicity analysis. Subsequently, BM-MSCs were induced to differentiate into adipocytes by photo-induced biomodulation with AlClPc/NE-LLL. Results: Our results showed that the isolated cell population was consistent with murine BM-MSCs. The cellular cytotoxicity analysis revealed that the optimal nanoemulsion dose to induce BM-MSC biomodulation was 5.0 μmol/L. Twenty-four hours following treatment with AlClPc/NE, BM-MSC were subjected to visible light irradiation of 20 mJ/cm 2 at 670 nm. Six days after photo-induced biomodulation, cells maintained high GFP expression level, and expressed detectable mRNA levels of adipogenic genes (lipoprotein lipase and PPARγ); formation of lipid vacuoles was observed, and the cells did not show any tumorigenic potential in vivo. Conclusions: Our results indicated that photo-induced biomodulation via visible light using AlClPc/NE and LLL can induce adipogenic differentiation of murine BM-MSCs. Therefore, cell therapy with BM-MSCs and photo-induced biomodulation may contribute to the development of new therapeutic strategies that are faster and more effective than traditional methods to trigger MSC differentiation. |
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Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulationAdipogenic differentiationBone marrow-mesenchymal stem cells (BM-MSC)Chloroaluminum phthalocyanine (AlClPc)Low level laser (LLL)Photo induced biomodulationBackground: Bone marrow mesenchymal stem cells (BM-MSCs) are undifferentiated cells that can proliferate and differentiate into specialized cells for tissue self-repair. Low-level laser (LLL) can induce biomodulatory effects such as cellular proliferation, differentiation, and migration. We investigated the biomodulatory effects of the photoactive compound chloroaluminum phthalocyanine nanoemulsion (AlClPc/NE) on the adipogenic differentiation of BM-MSCs, when combined with LLL (AlClPc/NE-LLL). Methods: The BM-MSCs used in this work were isolated from green fluorescent protein-positive (GFP + ) C57BL6 mice. Cells were first treated with AlClPc/NE, a well-designed photoactive nano-drug and were then subjected to in vitro expansion, morphological and immunophenotypic characterization, and cellular cytotoxicity analysis. Subsequently, BM-MSCs were induced to differentiate into adipocytes by photo-induced biomodulation with AlClPc/NE-LLL. Results: Our results showed that the isolated cell population was consistent with murine BM-MSCs. The cellular cytotoxicity analysis revealed that the optimal nanoemulsion dose to induce BM-MSC biomodulation was 5.0 μmol/L. Twenty-four hours following treatment with AlClPc/NE, BM-MSC were subjected to visible light irradiation of 20 mJ/cm 2 at 670 nm. Six days after photo-induced biomodulation, cells maintained high GFP expression level, and expressed detectable mRNA levels of adipogenic genes (lipoprotein lipase and PPARγ); formation of lipid vacuoles was observed, and the cells did not show any tumorigenic potential in vivo. Conclusions: Our results indicated that photo-induced biomodulation via visible light using AlClPc/NE and LLL can induce adipogenic differentiation of murine BM-MSCs. Therefore, cell therapy with BM-MSCs and photo-induced biomodulation may contribute to the development of new therapeutic strategies that are faster and more effective than traditional methods to trigger MSC differentiation.Financiadora de Estudos e ProjetosDepartment of Chemistry Center of Nanotechnology and Tissue Engineering – Photobiology and Photomedicine Research Group Faculty of Philosophy Sciences and Letters of Ribeirão Preto Ribeirão Preto University of São PauloCenter for Cell Therapy and Regional Blood Center National Institute of Science and Technology in Stem Cell and Cell Therapy Medical School University of São PauloDepartment of Pathology Medical School University of Sao PauloDepartament of Bioprocess and Biotechnology São Paulo State University (UNESP) School of Pharmaceutical SciencesDepartament of de Genetics Medical School University of São PauloDepartament of Bioprocess and Biotechnology São Paulo State University (UNESP) School of Pharmaceutical SciencesFinanciadora de Estudos e Projetos: 01.10.0758.01Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Castilho-Fernandes, AndrielleLopes, Tácila G.Ferreira, Fernanda U.Rezende, NayaraSilva, Valéria F.Primo, Fernando L. [UNESP]Fontes, Aparecida MariaRibeiro-Silva, AlfredoTedesco, Antonio Claudio2019-10-06T16:48:32Z2019-10-06T16:48:32Z2019-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article119-127http://dx.doi.org/10.1016/j.pdpdt.2018.11.013Photodiagnosis and Photodynamic Therapy, v. 25, p. 119-127.1873-15971572-1000http://hdl.handle.net/11449/18967610.1016/j.pdpdt.2018.11.0132-s2.0-85057557782Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2024-09-03T13:14:32Zoai:repositorio.unesp.br:11449/189676Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation |
title |
Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation |
spellingShingle |
Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation Castilho-Fernandes, Andrielle Adipogenic differentiation Bone marrow-mesenchymal stem cells (BM-MSC) Chloroaluminum phthalocyanine (AlClPc) Low level laser (LLL) Photo induced biomodulation |
title_short |
Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation |
title_full |
Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation |
title_fullStr |
Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation |
title_full_unstemmed |
Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation |
title_sort |
Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation |
author |
Castilho-Fernandes, Andrielle |
author_facet |
Castilho-Fernandes, Andrielle Lopes, Tácila G. Ferreira, Fernanda U. Rezende, Nayara Silva, Valéria F. Primo, Fernando L. [UNESP] Fontes, Aparecida Maria Ribeiro-Silva, Alfredo Tedesco, Antonio Claudio |
author_role |
author |
author2 |
Lopes, Tácila G. Ferreira, Fernanda U. Rezende, Nayara Silva, Valéria F. Primo, Fernando L. [UNESP] Fontes, Aparecida Maria Ribeiro-Silva, Alfredo Tedesco, Antonio Claudio |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Castilho-Fernandes, Andrielle Lopes, Tácila G. Ferreira, Fernanda U. Rezende, Nayara Silva, Valéria F. Primo, Fernando L. [UNESP] Fontes, Aparecida Maria Ribeiro-Silva, Alfredo Tedesco, Antonio Claudio |
dc.subject.por.fl_str_mv |
Adipogenic differentiation Bone marrow-mesenchymal stem cells (BM-MSC) Chloroaluminum phthalocyanine (AlClPc) Low level laser (LLL) Photo induced biomodulation |
topic |
Adipogenic differentiation Bone marrow-mesenchymal stem cells (BM-MSC) Chloroaluminum phthalocyanine (AlClPc) Low level laser (LLL) Photo induced biomodulation |
description |
Background: Bone marrow mesenchymal stem cells (BM-MSCs) are undifferentiated cells that can proliferate and differentiate into specialized cells for tissue self-repair. Low-level laser (LLL) can induce biomodulatory effects such as cellular proliferation, differentiation, and migration. We investigated the biomodulatory effects of the photoactive compound chloroaluminum phthalocyanine nanoemulsion (AlClPc/NE) on the adipogenic differentiation of BM-MSCs, when combined with LLL (AlClPc/NE-LLL). Methods: The BM-MSCs used in this work were isolated from green fluorescent protein-positive (GFP + ) C57BL6 mice. Cells were first treated with AlClPc/NE, a well-designed photoactive nano-drug and were then subjected to in vitro expansion, morphological and immunophenotypic characterization, and cellular cytotoxicity analysis. Subsequently, BM-MSCs were induced to differentiate into adipocytes by photo-induced biomodulation with AlClPc/NE-LLL. Results: Our results showed that the isolated cell population was consistent with murine BM-MSCs. The cellular cytotoxicity analysis revealed that the optimal nanoemulsion dose to induce BM-MSC biomodulation was 5.0 μmol/L. Twenty-four hours following treatment with AlClPc/NE, BM-MSC were subjected to visible light irradiation of 20 mJ/cm 2 at 670 nm. Six days after photo-induced biomodulation, cells maintained high GFP expression level, and expressed detectable mRNA levels of adipogenic genes (lipoprotein lipase and PPARγ); formation of lipid vacuoles was observed, and the cells did not show any tumorigenic potential in vivo. Conclusions: Our results indicated that photo-induced biomodulation via visible light using AlClPc/NE and LLL can induce adipogenic differentiation of murine BM-MSCs. Therefore, cell therapy with BM-MSCs and photo-induced biomodulation may contribute to the development of new therapeutic strategies that are faster and more effective than traditional methods to trigger MSC differentiation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:48:32Z 2019-10-06T16:48:32Z 2019-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2018.11.013 Photodiagnosis and Photodynamic Therapy, v. 25, p. 119-127. 1873-1597 1572-1000 http://hdl.handle.net/11449/189676 10.1016/j.pdpdt.2018.11.013 2-s2.0-85057557782 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2018.11.013 http://hdl.handle.net/11449/189676 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 25, p. 119-127. 1873-1597 1572-1000 10.1016/j.pdpdt.2018.11.013 2-s2.0-85057557782 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
119-127 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021365391032320 |