Photodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cells
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s43630-023-00382-9 http://hdl.handle.net/11449/249716 |
Resumo: | Prostate cancer is the most common cancer in American men, aside from skin cancer. As an alternative cancer treatment, photodynamic laser therapy (PDT) can be used to induce cell death. We evaluated the PDT effect, using methylene blue as a photosensitizer, in human prostate tumor cells (PC3). PC3 were subjected to four different conditions: DMEM (control); laser treatment (L—660 nm, 100 mW, 100 J.cm−2); methylene blue treatment (MB—25 μM, 30 min), and MB treatment followed by low-level red laser irradiation (MB-PDT). Groups were evaluated after 24 h. MB-PDT treatment reduced cell viability and migration. However, because MB-PDT did not significantly increase the levels of active caspase-3 and BCL-2, apoptosis was not the primary mode of cell death. MB-PDT, on the other hand, increased the acid compartment by 100% and the LC3 immunofluorescence (an autophagy marker) by 254%. Active MLKL level, a necroptosis marker, was higher in PC3 cells after MB-PDT treatment. Furthermore, MB-PDT resulted in oxidative stress due to a decrease in total antioxidant potential, catalase levels, and increased lipid peroxidation. According to these findings, MB-PDT therapy is effective at inducing oxidative stress and reducing PC3 cell viability. In such therapy, necroptosis is also an important mechanism of cell death triggered by autophagy. Graphical Abstract: [Figure not available: see fulltext.] |
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Photodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cellsCell deathMethylene bluePhotodynamic therapyProstate cancerProstate cancer is the most common cancer in American men, aside from skin cancer. As an alternative cancer treatment, photodynamic laser therapy (PDT) can be used to induce cell death. We evaluated the PDT effect, using methylene blue as a photosensitizer, in human prostate tumor cells (PC3). PC3 were subjected to four different conditions: DMEM (control); laser treatment (L—660 nm, 100 mW, 100 J.cm−2); methylene blue treatment (MB—25 μM, 30 min), and MB treatment followed by low-level red laser irradiation (MB-PDT). Groups were evaluated after 24 h. MB-PDT treatment reduced cell viability and migration. However, because MB-PDT did not significantly increase the levels of active caspase-3 and BCL-2, apoptosis was not the primary mode of cell death. MB-PDT, on the other hand, increased the acid compartment by 100% and the LC3 immunofluorescence (an autophagy marker) by 254%. Active MLKL level, a necroptosis marker, was higher in PC3 cells after MB-PDT treatment. Furthermore, MB-PDT resulted in oxidative stress due to a decrease in total antioxidant potential, catalase levels, and increased lipid peroxidation. According to these findings, MB-PDT therapy is effective at inducing oxidative stress and reducing PC3 cell viability. In such therapy, necroptosis is also an important mechanism of cell death triggered by autophagy. Graphical Abstract: [Figure not available: see fulltext.]Department of Cell Biology Histology and Embryology. Institute of Biomedical Sciences-ICBIM Federal University of Uberlândia-UFU, Minas GeraisDepartment of Anatomy. Institute of Biomedical Sciences-ICBIM Federal University of Uberlândia-UFU, Minas GeraisInstitute of Biotechnology-IBTEC Federal University of Uberlândia-UFU, Minas GeraisDepartment of Immunology Institute of Biomedical Sciences-ICBIM Federal University of Uberlândia-UFU, Minas GeraisFaculdade de Medicina União das Faculdades Dos Grandes Lagos São José Do Rio Preto-São PauloDepartment of Biology Institute of Biosciences Humanities and Exact Sciences São Paulo State University-UNESPDepartment of Morphology Institute of Biological Sciences Federal University of Juiz de Fora-UFJF, Minas GeraisDepartment of Biology Institute of Biosciences Humanities and Exact Sciences São Paulo State University-UNESPUniversidade Federal de Uberlândia (UFU)São José Do Rio Preto-São PauloUniversidade Estadual Paulista (UNESP)Federal University of Juiz de Fora-UFJFde Melo Gomes, Laura Calazansde Oliveira Cunha, Amanda BranquinhoPeixoto, Luiz Felipe FernandesZanon, Renata GracieleBotelho, Françoise VasconcelosSilva, Marcelo José BarbosaPinto-Fochi, Maria EtelvinaGóes, Rejane Maira [UNESP]de Paoli, FláviaRibeiro, Daniele Lisboa2023-07-29T16:07:18Z2023-07-29T16:07:18Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s43630-023-00382-9Photochemical and Photobiological Sciences.1474-90921474-905Xhttp://hdl.handle.net/11449/24971610.1007/s43630-023-00382-92-s2.0-85149204525Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotochemical and Photobiological Sciencesinfo:eu-repo/semantics/openAccess2023-07-29T16:07:18Zoai:repositorio.unesp.br:11449/249716Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:15:23.199088Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Photodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cells |
title |
Photodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cells |
spellingShingle |
Photodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cells de Melo Gomes, Laura Calazans Cell death Methylene blue Photodynamic therapy Prostate cancer |
title_short |
Photodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cells |
title_full |
Photodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cells |
title_fullStr |
Photodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cells |
title_full_unstemmed |
Photodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cells |
title_sort |
Photodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cells |
author |
de Melo Gomes, Laura Calazans |
author_facet |
de Melo Gomes, Laura Calazans de Oliveira Cunha, Amanda Branquinho Peixoto, Luiz Felipe Fernandes Zanon, Renata Graciele Botelho, Françoise Vasconcelos Silva, Marcelo José Barbosa Pinto-Fochi, Maria Etelvina Góes, Rejane Maira [UNESP] de Paoli, Flávia Ribeiro, Daniele Lisboa |
author_role |
author |
author2 |
de Oliveira Cunha, Amanda Branquinho Peixoto, Luiz Felipe Fernandes Zanon, Renata Graciele Botelho, Françoise Vasconcelos Silva, Marcelo José Barbosa Pinto-Fochi, Maria Etelvina Góes, Rejane Maira [UNESP] de Paoli, Flávia Ribeiro, Daniele Lisboa |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) São José Do Rio Preto-São Paulo Universidade Estadual Paulista (UNESP) Federal University of Juiz de Fora-UFJF |
dc.contributor.author.fl_str_mv |
de Melo Gomes, Laura Calazans de Oliveira Cunha, Amanda Branquinho Peixoto, Luiz Felipe Fernandes Zanon, Renata Graciele Botelho, Françoise Vasconcelos Silva, Marcelo José Barbosa Pinto-Fochi, Maria Etelvina Góes, Rejane Maira [UNESP] de Paoli, Flávia Ribeiro, Daniele Lisboa |
dc.subject.por.fl_str_mv |
Cell death Methylene blue Photodynamic therapy Prostate cancer |
topic |
Cell death Methylene blue Photodynamic therapy Prostate cancer |
description |
Prostate cancer is the most common cancer in American men, aside from skin cancer. As an alternative cancer treatment, photodynamic laser therapy (PDT) can be used to induce cell death. We evaluated the PDT effect, using methylene blue as a photosensitizer, in human prostate tumor cells (PC3). PC3 were subjected to four different conditions: DMEM (control); laser treatment (L—660 nm, 100 mW, 100 J.cm−2); methylene blue treatment (MB—25 μM, 30 min), and MB treatment followed by low-level red laser irradiation (MB-PDT). Groups were evaluated after 24 h. MB-PDT treatment reduced cell viability and migration. However, because MB-PDT did not significantly increase the levels of active caspase-3 and BCL-2, apoptosis was not the primary mode of cell death. MB-PDT, on the other hand, increased the acid compartment by 100% and the LC3 immunofluorescence (an autophagy marker) by 254%. Active MLKL level, a necroptosis marker, was higher in PC3 cells after MB-PDT treatment. Furthermore, MB-PDT resulted in oxidative stress due to a decrease in total antioxidant potential, catalase levels, and increased lipid peroxidation. According to these findings, MB-PDT therapy is effective at inducing oxidative stress and reducing PC3 cell viability. In such therapy, necroptosis is also an important mechanism of cell death triggered by autophagy. Graphical Abstract: [Figure not available: see fulltext.] |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:07:18Z 2023-07-29T16:07:18Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s43630-023-00382-9 Photochemical and Photobiological Sciences. 1474-9092 1474-905X http://hdl.handle.net/11449/249716 10.1007/s43630-023-00382-9 2-s2.0-85149204525 |
url |
http://dx.doi.org/10.1007/s43630-023-00382-9 http://hdl.handle.net/11449/249716 |
identifier_str_mv |
Photochemical and Photobiological Sciences. 1474-9092 1474-905X 10.1007/s43630-023-00382-9 2-s2.0-85149204525 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photochemical and Photobiological Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129043250282496 |