Preparation and in vitro evaluation of PLA/biphasic calcium phosphate filaments used for fused deposition modelling of scaffolds
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.msec.2020.111013 http://hdl.handle.net/11449/198812 |
Resumo: | Ceramic materials such as calcium phosphates (CaPs) with a composition similar to the mineral phase of bones and polymeric polylactic acid (PLA) are potential candidates for the manufacturing of scaffolds to act as bone substitutes and for tissue engineering applications, due to their bioresorbability and biocompatibility. Variables such as porosity, topography, morphology, and mechanical properties play an essential role in the scaffolds response. In this paper, a polymer/ceramic composite filament of 1.7 mm in diameter based on PLA and biphasic calcium phosphates (BCPs) was obtained by hot-melt extrusion in a single screw extruder. The particles of BCP were obtained by solution-combustion synthesis, and the PLA used was commercial grade. The BCPs ceramics were characterized by X-ray diffraction (XRD), scanning electron microscopic (SEM), transmission electron microscopy (TEM), and Brunauer, Emmett, and Teller (BET). It was possible to confirm that the main inorganic phases were hydroxyapatite (HAP) and tricalcium phosphate (TCP) with grain sizes below 100 nm and with high porosity. The Filaments obtained are a bit fragile but were able to be used in fused deposition modelling (FDM) using low-cost commercial printers. The filaments were characterized by SEM and energy dispersive X-ray (EDX). The in-vitro tests of filaments showed deposition of apatite phases on their surface, non-cytotoxic behavior, adequate cell proliferation and cell adhesion. |
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Preparation and in vitro evaluation of PLA/biphasic calcium phosphate filaments used for fused deposition modelling of scaffoldsBone substitutesCalcium phosphatesCompositesFDMFilamentsScaffoldsCeramic materials such as calcium phosphates (CaPs) with a composition similar to the mineral phase of bones and polymeric polylactic acid (PLA) are potential candidates for the manufacturing of scaffolds to act as bone substitutes and for tissue engineering applications, due to their bioresorbability and biocompatibility. Variables such as porosity, topography, morphology, and mechanical properties play an essential role in the scaffolds response. In this paper, a polymer/ceramic composite filament of 1.7 mm in diameter based on PLA and biphasic calcium phosphates (BCPs) was obtained by hot-melt extrusion in a single screw extruder. The particles of BCP were obtained by solution-combustion synthesis, and the PLA used was commercial grade. The BCPs ceramics were characterized by X-ray diffraction (XRD), scanning electron microscopic (SEM), transmission electron microscopy (TEM), and Brunauer, Emmett, and Teller (BET). It was possible to confirm that the main inorganic phases were hydroxyapatite (HAP) and tricalcium phosphate (TCP) with grain sizes below 100 nm and with high porosity. The Filaments obtained are a bit fragile but were able to be used in fused deposition modelling (FDM) using low-cost commercial printers. The filaments were characterized by SEM and energy dispersive X-ray (EDX). The in-vitro tests of filaments showed deposition of apatite phases on their surface, non-cytotoxic behavior, adequate cell proliferation and cell adhesion.Departamento Administrativo de Ciencia, Tecnología e Innovación (COLCIENCIAS)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Grupo de Materiales Cerámicos y Vítreos Escuela de Física Universidad Nacional de Colombia, Calle 59A.63-20Center for Natural and Human Sciences (CCNH) Federal University of ABC (UFABC)Grupo GICEI Institución Universitaria Pascual Bravo, Calle 73 No. 73A – 226LIEC Institute of Chemistry São Paulo State University-UNESPUniversidad de Antioquia PECET-Instituto de Investigaciones Médicas Facultad de Medicina, Calle 62 No. 52-59Grupo de Tribología y Superficies Departamento de Materiales Universidad Nacional de Colombia Sede Medellín, Calle 59A.63-20LIEC Institute of Chemistry São Paulo State University-UNESPEscuela de Física Universidad Nacional de ColombiaUniversidade Federal do ABC (UFABC)Institución Universitaria Pascual BravoUniversidade Estadual Paulista (Unesp)Facultad de MedicinaSede MedellínNevado, P.Lopera, A.Bezzon, V.Fulla, M. R.Palacio, J.Zaghete, M. A. [UNESP]Biasotto, G. [UNESP]Montoya, A.Rivera, J.Robledo, S. M.Estupiñan, H.Paucar, C.Garcia, C.2020-12-12T01:22:39Z2020-12-12T01:22:39Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.msec.2020.111013Materials Science and Engineering C, v. 114.1873-01910928-4931http://hdl.handle.net/11449/19881210.1016/j.msec.2020.1110132-s2.0-85084339032Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science and Engineering Cinfo:eu-repo/semantics/openAccess2021-10-22T20:36:07Zoai:repositorio.unesp.br:11449/198812Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T20:36:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Preparation and in vitro evaluation of PLA/biphasic calcium phosphate filaments used for fused deposition modelling of scaffolds |
title |
Preparation and in vitro evaluation of PLA/biphasic calcium phosphate filaments used for fused deposition modelling of scaffolds |
spellingShingle |
Preparation and in vitro evaluation of PLA/biphasic calcium phosphate filaments used for fused deposition modelling of scaffolds Nevado, P. Bone substitutes Calcium phosphates Composites FDM Filaments Scaffolds |
title_short |
Preparation and in vitro evaluation of PLA/biphasic calcium phosphate filaments used for fused deposition modelling of scaffolds |
title_full |
Preparation and in vitro evaluation of PLA/biphasic calcium phosphate filaments used for fused deposition modelling of scaffolds |
title_fullStr |
Preparation and in vitro evaluation of PLA/biphasic calcium phosphate filaments used for fused deposition modelling of scaffolds |
title_full_unstemmed |
Preparation and in vitro evaluation of PLA/biphasic calcium phosphate filaments used for fused deposition modelling of scaffolds |
title_sort |
Preparation and in vitro evaluation of PLA/biphasic calcium phosphate filaments used for fused deposition modelling of scaffolds |
author |
Nevado, P. |
author_facet |
Nevado, P. Lopera, A. Bezzon, V. Fulla, M. R. Palacio, J. Zaghete, M. A. [UNESP] Biasotto, G. [UNESP] Montoya, A. Rivera, J. Robledo, S. M. Estupiñan, H. Paucar, C. Garcia, C. |
author_role |
author |
author2 |
Lopera, A. Bezzon, V. Fulla, M. R. Palacio, J. Zaghete, M. A. [UNESP] Biasotto, G. [UNESP] Montoya, A. Rivera, J. Robledo, S. M. Estupiñan, H. Paucar, C. Garcia, C. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Escuela de Física Universidad Nacional de Colombia Universidade Federal do ABC (UFABC) Institución Universitaria Pascual Bravo Universidade Estadual Paulista (Unesp) Facultad de Medicina Sede Medellín |
dc.contributor.author.fl_str_mv |
Nevado, P. Lopera, A. Bezzon, V. Fulla, M. R. Palacio, J. Zaghete, M. A. [UNESP] Biasotto, G. [UNESP] Montoya, A. Rivera, J. Robledo, S. M. Estupiñan, H. Paucar, C. Garcia, C. |
dc.subject.por.fl_str_mv |
Bone substitutes Calcium phosphates Composites FDM Filaments Scaffolds |
topic |
Bone substitutes Calcium phosphates Composites FDM Filaments Scaffolds |
description |
Ceramic materials such as calcium phosphates (CaPs) with a composition similar to the mineral phase of bones and polymeric polylactic acid (PLA) are potential candidates for the manufacturing of scaffolds to act as bone substitutes and for tissue engineering applications, due to their bioresorbability and biocompatibility. Variables such as porosity, topography, morphology, and mechanical properties play an essential role in the scaffolds response. In this paper, a polymer/ceramic composite filament of 1.7 mm in diameter based on PLA and biphasic calcium phosphates (BCPs) was obtained by hot-melt extrusion in a single screw extruder. The particles of BCP were obtained by solution-combustion synthesis, and the PLA used was commercial grade. The BCPs ceramics were characterized by X-ray diffraction (XRD), scanning electron microscopic (SEM), transmission electron microscopy (TEM), and Brunauer, Emmett, and Teller (BET). It was possible to confirm that the main inorganic phases were hydroxyapatite (HAP) and tricalcium phosphate (TCP) with grain sizes below 100 nm and with high porosity. The Filaments obtained are a bit fragile but were able to be used in fused deposition modelling (FDM) using low-cost commercial printers. The filaments were characterized by SEM and energy dispersive X-ray (EDX). The in-vitro tests of filaments showed deposition of apatite phases on their surface, non-cytotoxic behavior, adequate cell proliferation and cell adhesion. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:22:39Z 2020-12-12T01:22:39Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.msec.2020.111013 Materials Science and Engineering C, v. 114. 1873-0191 0928-4931 http://hdl.handle.net/11449/198812 10.1016/j.msec.2020.111013 2-s2.0-85084339032 |
url |
http://dx.doi.org/10.1016/j.msec.2020.111013 http://hdl.handle.net/11449/198812 |
identifier_str_mv |
Materials Science and Engineering C, v. 114. 1873-0191 0928-4931 10.1016/j.msec.2020.111013 2-s2.0-85084339032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Materials Science and Engineering C |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797789749166997504 |