Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzi
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0102-695X2012005000014 http://hdl.handle.net/11449/26311 |
Resumo: | Chagas disease is one of the main public health problems in Latin America. Since the available treatments for this disease are not effective in providing cure, the screening of potential antiprotozoal agents is essential, mainly of those obtained from natural sources. This study aimed to provide an evaluation of the trypanocidal activity of 92 ethanol extracts from species belonging to the families Annonaceae, Apiaceae, Cucurbitaceae, Lamiaceae, Lauraceae, Moraceae, Nyctaginaceae, and Verbenaceae against the Y and Bolivia strains of Trypanosoma cruzi. Additionally, cytotoxic activity on LLCMK2 fibroblasts was evaluated. Both the trypanocidal activity and cytotoxicity were evaluated using the MTT method, in the following concentrations: 500, 350, 250, and 100 µg/mL. Benznidazole was used for positive control. The best results among the 92 samples evaluated were obtained with ethanol extracts of Ocotea paranapiacabensis (Am93) and Aegiphila lhotzkiana (Am160). Am93 showed trypanocidal activity against epimastigote forms of the Bolivia strain and was moderately toxic to LLCMK2 cells, its Selectivity Index (SI) being 14.56, while Am160 showed moderate trypanocidal activity against the Bolivia strain and moderate toxicicity, its SI being equal to 1.15. The screening of Brazilian plants has indicated the potential effect of ethanol extracts obtained from Ocotea paranapiacabensis and Aegiphila lhotzkiana against Chagas disease. |
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Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzibiodiversityChagascytotoxicitydiseasestrainstrypanocidal activityTrypanosoma cruziChagas disease is one of the main public health problems in Latin America. Since the available treatments for this disease are not effective in providing cure, the screening of potential antiprotozoal agents is essential, mainly of those obtained from natural sources. This study aimed to provide an evaluation of the trypanocidal activity of 92 ethanol extracts from species belonging to the families Annonaceae, Apiaceae, Cucurbitaceae, Lamiaceae, Lauraceae, Moraceae, Nyctaginaceae, and Verbenaceae against the Y and Bolivia strains of Trypanosoma cruzi. Additionally, cytotoxic activity on LLCMK2 fibroblasts was evaluated. Both the trypanocidal activity and cytotoxicity were evaluated using the MTT method, in the following concentrations: 500, 350, 250, and 100 µg/mL. Benznidazole was used for positive control. The best results among the 92 samples evaluated were obtained with ethanol extracts of Ocotea paranapiacabensis (Am93) and Aegiphila lhotzkiana (Am160). Am93 showed trypanocidal activity against epimastigote forms of the Bolivia strain and was moderately toxic to LLCMK2 cells, its Selectivity Index (SI) being 14.56, while Am160 showed moderate trypanocidal activity against the Bolivia strain and moderate toxicicity, its SI being equal to 1.15. The screening of Brazilian plants has indicated the potential effect of ethanol extracts obtained from Ocotea paranapiacabensis and Aegiphila lhotzkiana against Chagas disease.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista Faculdade de Ciências Farmacêuticas de Araraquara-SP Laboratório de ParasitologiaUniversidade Estadual Paulista Instituto de Química de Araraquara-SP Departamento de Química OrgânicaInstituto de Botânica de São PauloUniversidade Estadual de Campinas-SP Instituto de BiologiaUniversidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto-SP Laboratório de ParasitologiaUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas de Araraquara-SP Laboratório de ParasitologiaUniversidade Estadual Paulista Instituto de Química de Araraquara-SP Departamento de Química OrgânicaFAPESP: 03/02176-7FAPESP: 04/07932Sociedade Brasileira de FarmacognosiaUniversidade Estadual Paulista (Unesp)Instituto de Botânica de São PauloUniversidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Alves, Renata Tomé [UNESP]Regasini, Luis Octávio [UNESP]Funari, Cristiano Soleo [UNESP]Young, Maria Cláudia MarxRimoldi, AlineBolzani, Vanderlan da Silva [UNESP]Silva, Dulce Helena Siqueira [UNESP]Albuquerque, Sérgio deRosa, João Aristeu da [UNESP]2014-05-20T14:21:05Z2014-05-20T14:21:05Z2012-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article528-534application/pdfhttp://dx.doi.org/10.1590/S0102-695X2012005000014Revista Brasileira de Farmacognosia. Sociedade Brasileira de Farmacognosia, v. 22, n. 3, p. 528-534, 2012.0102-695Xhttp://hdl.handle.net/11449/2631110.1590/S0102-695X2012005000014S0102-695X2012000300010WOS:000304083100010S0102-695X2012000300010.pdf4484083685251673470200490423124809927364527645500000-0002-1516-7765SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Farmacognosia1.596info:eu-repo/semantics/openAccess2024-01-15T06:19:53Zoai:repositorio.unesp.br:11449/26311Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-15T06:19:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzi |
title |
Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzi |
spellingShingle |
Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzi Alves, Renata Tomé [UNESP] biodiversity Chagas cytotoxicity disease strains trypanocidal activity Trypanosoma cruzi |
title_short |
Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzi |
title_full |
Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzi |
title_fullStr |
Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzi |
title_full_unstemmed |
Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzi |
title_sort |
Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanosoma cruzi |
author |
Alves, Renata Tomé [UNESP] |
author_facet |
Alves, Renata Tomé [UNESP] Regasini, Luis Octávio [UNESP] Funari, Cristiano Soleo [UNESP] Young, Maria Cláudia Marx Rimoldi, Aline Bolzani, Vanderlan da Silva [UNESP] Silva, Dulce Helena Siqueira [UNESP] Albuquerque, Sérgio de Rosa, João Aristeu da [UNESP] |
author_role |
author |
author2 |
Regasini, Luis Octávio [UNESP] Funari, Cristiano Soleo [UNESP] Young, Maria Cláudia Marx Rimoldi, Aline Bolzani, Vanderlan da Silva [UNESP] Silva, Dulce Helena Siqueira [UNESP] Albuquerque, Sérgio de Rosa, João Aristeu da [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto de Botânica de São Paulo Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Alves, Renata Tomé [UNESP] Regasini, Luis Octávio [UNESP] Funari, Cristiano Soleo [UNESP] Young, Maria Cláudia Marx Rimoldi, Aline Bolzani, Vanderlan da Silva [UNESP] Silva, Dulce Helena Siqueira [UNESP] Albuquerque, Sérgio de Rosa, João Aristeu da [UNESP] |
dc.subject.por.fl_str_mv |
biodiversity Chagas cytotoxicity disease strains trypanocidal activity Trypanosoma cruzi |
topic |
biodiversity Chagas cytotoxicity disease strains trypanocidal activity Trypanosoma cruzi |
description |
Chagas disease is one of the main public health problems in Latin America. Since the available treatments for this disease are not effective in providing cure, the screening of potential antiprotozoal agents is essential, mainly of those obtained from natural sources. This study aimed to provide an evaluation of the trypanocidal activity of 92 ethanol extracts from species belonging to the families Annonaceae, Apiaceae, Cucurbitaceae, Lamiaceae, Lauraceae, Moraceae, Nyctaginaceae, and Verbenaceae against the Y and Bolivia strains of Trypanosoma cruzi. Additionally, cytotoxic activity on LLCMK2 fibroblasts was evaluated. Both the trypanocidal activity and cytotoxicity were evaluated using the MTT method, in the following concentrations: 500, 350, 250, and 100 µg/mL. Benznidazole was used for positive control. The best results among the 92 samples evaluated were obtained with ethanol extracts of Ocotea paranapiacabensis (Am93) and Aegiphila lhotzkiana (Am160). Am93 showed trypanocidal activity against epimastigote forms of the Bolivia strain and was moderately toxic to LLCMK2 cells, its Selectivity Index (SI) being 14.56, while Am160 showed moderate trypanocidal activity against the Bolivia strain and moderate toxicicity, its SI being equal to 1.15. The screening of Brazilian plants has indicated the potential effect of ethanol extracts obtained from Ocotea paranapiacabensis and Aegiphila lhotzkiana against Chagas disease. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 2014-05-20T14:21:05Z 2014-05-20T14:21:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-695X2012005000014 Revista Brasileira de Farmacognosia. Sociedade Brasileira de Farmacognosia, v. 22, n. 3, p. 528-534, 2012. 0102-695X http://hdl.handle.net/11449/26311 10.1590/S0102-695X2012005000014 S0102-695X2012000300010 WOS:000304083100010 S0102-695X2012000300010.pdf 4484083685251673 4702004904231248 0992736452764550 0000-0002-1516-7765 |
url |
http://dx.doi.org/10.1590/S0102-695X2012005000014 http://hdl.handle.net/11449/26311 |
identifier_str_mv |
Revista Brasileira de Farmacognosia. Sociedade Brasileira de Farmacognosia, v. 22, n. 3, p. 528-534, 2012. 0102-695X 10.1590/S0102-695X2012005000014 S0102-695X2012000300010 WOS:000304083100010 S0102-695X2012000300010.pdf 4484083685251673 4702004904231248 0992736452764550 0000-0002-1516-7765 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Farmacognosia 1.596 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
528-534 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047336501313536 |