Comparative evaluation of vegetable matter involved lesions with oral parasitic infections in the oral cavity
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jemt.24005 http://hdl.handle.net/11449/233860 |
Resumo: | The current research aims to perform a comparative evaluation of vegetable matter involved lesions with oral parasitic infections found in oral mucosa, presenting histochemical methods to differentiate their microscopic similarities. Eight cases were selected out of a sample of 1.975 reports from a single Oral and Maxillofacial Pathology Service of the author's institution from 2012 to 2019. Specimens were examined by hematoxylin and eosin staining (HE), periodic acid-Schiff (PAS) staining, Gomori–Grocott staining, Ziehl–Neelsen staining, Giemsa, and mucicarmine staining. Microscopic analysis included fluorescence, polarized light, and confocal microscopy. Microscopically, in HE coloration, hookworm eggs showed as eosinophilic. Inflammatory multinucleated giant cells and lymphocytes, were usually related to the nematode eggs, forming an intense inflammatory infiltrate. Biofluorescent properties of eggs and larvae revealed to be sensitive in the detection of parasitic structures contrasting with the inflamed connective tissue. Vegetable presence was confirmed by polarized light microscopy and it was found to be associated with microbial biofilms. Confocal microscopy has showed to be an excellent method for morphotype differentiation of parasitic eggs. Parasitic infection and vegetable matter displayed similarities in the inflammatory response, but the latter can rot and agglomerate biofilms. The microscopic diagnosis of such infections requires the interpretation of challenging morphological features since the parasites are usually sectioned and mixed with an inflammatory reaction. These histochemical approaches proved to be excellent to distinguish both lesions. |
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Comparative evaluation of vegetable matter involved lesions with oral parasitic infections in the oral cavityancylostomiasisdenturehookwormoral parasitevegetable matterThe current research aims to perform a comparative evaluation of vegetable matter involved lesions with oral parasitic infections found in oral mucosa, presenting histochemical methods to differentiate their microscopic similarities. Eight cases were selected out of a sample of 1.975 reports from a single Oral and Maxillofacial Pathology Service of the author's institution from 2012 to 2019. Specimens were examined by hematoxylin and eosin staining (HE), periodic acid-Schiff (PAS) staining, Gomori–Grocott staining, Ziehl–Neelsen staining, Giemsa, and mucicarmine staining. Microscopic analysis included fluorescence, polarized light, and confocal microscopy. Microscopically, in HE coloration, hookworm eggs showed as eosinophilic. Inflammatory multinucleated giant cells and lymphocytes, were usually related to the nematode eggs, forming an intense inflammatory infiltrate. Biofluorescent properties of eggs and larvae revealed to be sensitive in the detection of parasitic structures contrasting with the inflamed connective tissue. Vegetable presence was confirmed by polarized light microscopy and it was found to be associated with microbial biofilms. Confocal microscopy has showed to be an excellent method for morphotype differentiation of parasitic eggs. Parasitic infection and vegetable matter displayed similarities in the inflammatory response, but the latter can rot and agglomerate biofilms. The microscopic diagnosis of such infections requires the interpretation of challenging morphological features since the parasites are usually sectioned and mixed with an inflammatory reaction. These histochemical approaches proved to be excellent to distinguish both lesions.Fundação de Amparo à Pesquisa do Estado do AmazonasSchool of Health Sciences Amazonas State University, AMFaculty of Dentistry São Paulo State University, SPAmazon Hematology and Hemotherapy Foundation, AMFaculty of Dentistry São Paulo State University, SPAmazonas State UniversityUniversidade Estadual Paulista (UNESP)Amazon Hematology and Hemotherapy FoundationPinheiro, Tiago NovaesLeite, Milena Gomes Meloda Silva, Cristiane Cantiga [UNESP]Alexandre, Cleber NunesCabral, Lioney NobreCarvalho, Hannah Marcelle Paulainde Souza, Daniel FrotaGonçalves, Jéssica Lourdes de Aguiarde Souza, Thales Edecherly NasseralaMelo, Nara Deise de SouzaCintra, Luciano Tavares Ângelo [UNESP]Kanehira, Beatriz Terumi Barretode Albuquerque, Gustavo Cavalcanti2022-05-01T11:07:21Z2022-05-01T11:07:21Z2022-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1421-1432http://dx.doi.org/10.1002/jemt.24005Microscopy Research and Technique, v. 85, n. 4, p. 1421-1432, 2022.1097-00291059-910Xhttp://hdl.handle.net/11449/23386010.1002/jemt.240052-s2.0-85120429343Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroscopy Research and Techniqueinfo:eu-repo/semantics/openAccess2022-05-01T11:07:21Zoai:repositorio.unesp.br:11449/233860Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:11:44.222715Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparative evaluation of vegetable matter involved lesions with oral parasitic infections in the oral cavity |
title |
Comparative evaluation of vegetable matter involved lesions with oral parasitic infections in the oral cavity |
spellingShingle |
Comparative evaluation of vegetable matter involved lesions with oral parasitic infections in the oral cavity Pinheiro, Tiago Novaes ancylostomiasis denture hookworm oral parasite vegetable matter |
title_short |
Comparative evaluation of vegetable matter involved lesions with oral parasitic infections in the oral cavity |
title_full |
Comparative evaluation of vegetable matter involved lesions with oral parasitic infections in the oral cavity |
title_fullStr |
Comparative evaluation of vegetable matter involved lesions with oral parasitic infections in the oral cavity |
title_full_unstemmed |
Comparative evaluation of vegetable matter involved lesions with oral parasitic infections in the oral cavity |
title_sort |
Comparative evaluation of vegetable matter involved lesions with oral parasitic infections in the oral cavity |
author |
Pinheiro, Tiago Novaes |
author_facet |
Pinheiro, Tiago Novaes Leite, Milena Gomes Melo da Silva, Cristiane Cantiga [UNESP] Alexandre, Cleber Nunes Cabral, Lioney Nobre Carvalho, Hannah Marcelle Paulain de Souza, Daniel Frota Gonçalves, Jéssica Lourdes de Aguiar de Souza, Thales Edecherly Nasserala Melo, Nara Deise de Souza Cintra, Luciano Tavares Ângelo [UNESP] Kanehira, Beatriz Terumi Barreto de Albuquerque, Gustavo Cavalcanti |
author_role |
author |
author2 |
Leite, Milena Gomes Melo da Silva, Cristiane Cantiga [UNESP] Alexandre, Cleber Nunes Cabral, Lioney Nobre Carvalho, Hannah Marcelle Paulain de Souza, Daniel Frota Gonçalves, Jéssica Lourdes de Aguiar de Souza, Thales Edecherly Nasserala Melo, Nara Deise de Souza Cintra, Luciano Tavares Ângelo [UNESP] Kanehira, Beatriz Terumi Barreto de Albuquerque, Gustavo Cavalcanti |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Amazonas State University Universidade Estadual Paulista (UNESP) Amazon Hematology and Hemotherapy Foundation |
dc.contributor.author.fl_str_mv |
Pinheiro, Tiago Novaes Leite, Milena Gomes Melo da Silva, Cristiane Cantiga [UNESP] Alexandre, Cleber Nunes Cabral, Lioney Nobre Carvalho, Hannah Marcelle Paulain de Souza, Daniel Frota Gonçalves, Jéssica Lourdes de Aguiar de Souza, Thales Edecherly Nasserala Melo, Nara Deise de Souza Cintra, Luciano Tavares Ângelo [UNESP] Kanehira, Beatriz Terumi Barreto de Albuquerque, Gustavo Cavalcanti |
dc.subject.por.fl_str_mv |
ancylostomiasis denture hookworm oral parasite vegetable matter |
topic |
ancylostomiasis denture hookworm oral parasite vegetable matter |
description |
The current research aims to perform a comparative evaluation of vegetable matter involved lesions with oral parasitic infections found in oral mucosa, presenting histochemical methods to differentiate their microscopic similarities. Eight cases were selected out of a sample of 1.975 reports from a single Oral and Maxillofacial Pathology Service of the author's institution from 2012 to 2019. Specimens were examined by hematoxylin and eosin staining (HE), periodic acid-Schiff (PAS) staining, Gomori–Grocott staining, Ziehl–Neelsen staining, Giemsa, and mucicarmine staining. Microscopic analysis included fluorescence, polarized light, and confocal microscopy. Microscopically, in HE coloration, hookworm eggs showed as eosinophilic. Inflammatory multinucleated giant cells and lymphocytes, were usually related to the nematode eggs, forming an intense inflammatory infiltrate. Biofluorescent properties of eggs and larvae revealed to be sensitive in the detection of parasitic structures contrasting with the inflamed connective tissue. Vegetable presence was confirmed by polarized light microscopy and it was found to be associated with microbial biofilms. Confocal microscopy has showed to be an excellent method for morphotype differentiation of parasitic eggs. Parasitic infection and vegetable matter displayed similarities in the inflammatory response, but the latter can rot and agglomerate biofilms. The microscopic diagnosis of such infections requires the interpretation of challenging morphological features since the parasites are usually sectioned and mixed with an inflammatory reaction. These histochemical approaches proved to be excellent to distinguish both lesions. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01T11:07:21Z 2022-05-01T11:07:21Z 2022-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jemt.24005 Microscopy Research and Technique, v. 85, n. 4, p. 1421-1432, 2022. 1097-0029 1059-910X http://hdl.handle.net/11449/233860 10.1002/jemt.24005 2-s2.0-85120429343 |
url |
http://dx.doi.org/10.1002/jemt.24005 http://hdl.handle.net/11449/233860 |
identifier_str_mv |
Microscopy Research and Technique, v. 85, n. 4, p. 1421-1432, 2022. 1097-0029 1059-910X 10.1002/jemt.24005 2-s2.0-85120429343 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microscopy Research and Technique |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1421-1432 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129402965327872 |