Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions

Detalhes bibliográficos
Autor(a) principal: Souza, Maria Gorete Mendes
Data de Publicação: 2010
Outros Autores: Sato, Daisy Nakamura, Leite, Clarice Queiko Fujimura [UNESP], Leite, Sérgio Roberto de Andrade [UNESP], Sartori, Flávio Garcia, Prince, Karina de Andrade [UNESP], Casmeiro, Luciana Assirati, Martins, Carlos Henrique Gomes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.dovepress.com/occurrence-of-environmental-mycobacteria-pathogenic-on-surfaces-in-hea-peer-reviewed-article-RRTM
http://hdl.handle.net/11449/123639
Resumo: The presence of environmental mycobacteria on surfaces in two public health institutions, namely a health center and a hospital in upstate São Paulo (Brazil), was identified by polymerase chain reaction-restriction enzyme analysis (PRA). The possible sources of contamination by these microorganisms were evaluated, contributing to epidemiology studies. Methods: From June 2005 to June 2006, a total of 632 samples were collected from exposed surfaces, such as washbasins, drinking fountains, and other accessible sites, and the mycobacteria present in the samples were isolated and cultured. Results: Sixty-five mycobacteria were isolated from the 632 samples; 47 of which were detected in samples from the health center and 18 in samples collected from the hospital. The isolates were identified by DNA restriction patterns obtained by PRA, and potentially pathogenic species were found to be prevalent among the identified mycobacteria. This study shows that the PRA technique can be employed as a fast and easy method for identification of nontuberculous mycobacteria in public areas. Conclusions: The isolation of environmental mycobacteria from the two health institutions demonstrates that these surfaces are reservoirs of potentially pathogenic mycobacteria and indicates the need for continuous maintenance and monitoring. These data will add to the study of the epidemiology of these microorganisms.
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spelling Occurrence of pathogenic environmental mycobacteria on surfaces in health institutionsEnvironmental mycobacteriaHealth centerPCRPRAThe presence of environmental mycobacteria on surfaces in two public health institutions, namely a health center and a hospital in upstate São Paulo (Brazil), was identified by polymerase chain reaction-restriction enzyme analysis (PRA). The possible sources of contamination by these microorganisms were evaluated, contributing to epidemiology studies. Methods: From June 2005 to June 2006, a total of 632 samples were collected from exposed surfaces, such as washbasins, drinking fountains, and other accessible sites, and the mycobacteria present in the samples were isolated and cultured. Results: Sixty-five mycobacteria were isolated from the 632 samples; 47 of which were detected in samples from the health center and 18 in samples collected from the hospital. The isolates were identified by DNA restriction patterns obtained by PRA, and potentially pathogenic species were found to be prevalent among the identified mycobacteria. This study shows that the PRA technique can be employed as a fast and easy method for identification of nontuberculous mycobacteria in public areas. Conclusions: The isolation of environmental mycobacteria from the two health institutions demonstrates that these surfaces are reservoirs of potentially pathogenic mycobacteria and indicates the need for continuous maintenance and monitoring. These data will add to the study of the epidemiology of these microorganisms.Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química Geral e Inorgânica, Instituto de Química de Araraquara, Araraquara, Rua Professor Francisco Degni, 55, Jardim Quitandinha, CEP 14800060, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento Ciências Biológicas, Faculdade de Ciências Farmacêuticas de AraraquaraUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química Geral e Inorgânica, Instituto de Química de AraraquaraUniversidade de FrancaInstituto Adolfo LutzUniversidade Estadual Paulista (Unesp)Souza, Maria Gorete MendesSato, Daisy NakamuraLeite, Clarice Queiko Fujimura [UNESP]Leite, Sérgio Roberto de Andrade [UNESP]Sartori, Flávio GarciaPrince, Karina de Andrade [UNESP]Casmeiro, Luciana AssiratiMartins, Carlos Henrique Gomes2015-05-15T13:30:33Z2015-05-15T13:30:33Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article53-58application/pdfhttp://www.dovepress.com/occurrence-of-environmental-mycobacteria-pathogenic-on-surfaces-in-hea-peer-reviewed-article-RRTMResearch and Reports in Tropical Medicine, v. 2010, n. 1, p. 53-58, 2010.1179-7282http://hdl.handle.net/11449/12363910.2147/RRTM.S13589ISSN1179-7282-2010-2010-01-53-58.pdf81766663437672466420877890381807Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch and Reports in Tropical Medicineinfo:eu-repo/semantics/openAccess2024-06-24T13:08:25Zoai:repositorio.unesp.br:11449/123639Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:41:28.806003Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions
title Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions
spellingShingle Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions
Souza, Maria Gorete Mendes
Environmental mycobacteria
Health center
PCR
PRA
title_short Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions
title_full Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions
title_fullStr Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions
title_full_unstemmed Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions
title_sort Occurrence of pathogenic environmental mycobacteria on surfaces in health institutions
author Souza, Maria Gorete Mendes
author_facet Souza, Maria Gorete Mendes
Sato, Daisy Nakamura
Leite, Clarice Queiko Fujimura [UNESP]
Leite, Sérgio Roberto de Andrade [UNESP]
Sartori, Flávio Garcia
Prince, Karina de Andrade [UNESP]
Casmeiro, Luciana Assirati
Martins, Carlos Henrique Gomes
author_role author
author2 Sato, Daisy Nakamura
Leite, Clarice Queiko Fujimura [UNESP]
Leite, Sérgio Roberto de Andrade [UNESP]
Sartori, Flávio Garcia
Prince, Karina de Andrade [UNESP]
Casmeiro, Luciana Assirati
Martins, Carlos Henrique Gomes
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Franca
Instituto Adolfo Lutz
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Souza, Maria Gorete Mendes
Sato, Daisy Nakamura
Leite, Clarice Queiko Fujimura [UNESP]
Leite, Sérgio Roberto de Andrade [UNESP]
Sartori, Flávio Garcia
Prince, Karina de Andrade [UNESP]
Casmeiro, Luciana Assirati
Martins, Carlos Henrique Gomes
dc.subject.por.fl_str_mv Environmental mycobacteria
Health center
PCR
PRA
topic Environmental mycobacteria
Health center
PCR
PRA
description The presence of environmental mycobacteria on surfaces in two public health institutions, namely a health center and a hospital in upstate São Paulo (Brazil), was identified by polymerase chain reaction-restriction enzyme analysis (PRA). The possible sources of contamination by these microorganisms were evaluated, contributing to epidemiology studies. Methods: From June 2005 to June 2006, a total of 632 samples were collected from exposed surfaces, such as washbasins, drinking fountains, and other accessible sites, and the mycobacteria present in the samples were isolated and cultured. Results: Sixty-five mycobacteria were isolated from the 632 samples; 47 of which were detected in samples from the health center and 18 in samples collected from the hospital. The isolates were identified by DNA restriction patterns obtained by PRA, and potentially pathogenic species were found to be prevalent among the identified mycobacteria. This study shows that the PRA technique can be employed as a fast and easy method for identification of nontuberculous mycobacteria in public areas. Conclusions: The isolation of environmental mycobacteria from the two health institutions demonstrates that these surfaces are reservoirs of potentially pathogenic mycobacteria and indicates the need for continuous maintenance and monitoring. These data will add to the study of the epidemiology of these microorganisms.
publishDate 2010
dc.date.none.fl_str_mv 2010
2015-05-15T13:30:33Z
2015-05-15T13:30:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.dovepress.com/occurrence-of-environmental-mycobacteria-pathogenic-on-surfaces-in-hea-peer-reviewed-article-RRTM
Research and Reports in Tropical Medicine, v. 2010, n. 1, p. 53-58, 2010.
1179-7282
http://hdl.handle.net/11449/123639
10.2147/RRTM.S13589
ISSN1179-7282-2010-2010-01-53-58.pdf
8176666343767246
6420877890381807
url http://www.dovepress.com/occurrence-of-environmental-mycobacteria-pathogenic-on-surfaces-in-hea-peer-reviewed-article-RRTM
http://hdl.handle.net/11449/123639
identifier_str_mv Research and Reports in Tropical Medicine, v. 2010, n. 1, p. 53-58, 2010.
1179-7282
10.2147/RRTM.S13589
ISSN1179-7282-2010-2010-01-53-58.pdf
8176666343767246
6420877890381807
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Research and Reports in Tropical Medicine
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 53-58
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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