Automatic system for monitoring gaseous concentration in a packed-bed solid-state cultivation bioreactor

Detalhes bibliográficos
Autor(a) principal: Henrique, João Paulo [UNESP]
Data de Publicação: 2022
Outros Autores: Casciatori, Fernanda Perpétua, Thoméo, João Cláudio [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.ces.2022.117793
http://hdl.handle.net/11449/241963
Resumo: In this work, the respiration gases were sampled from a packed-bed bioreactor used for solid-state cultivation (SSC) using different gas-sampling procedures. As a case study, Myceliophthora thermophila I-1D3b was cultivated at 45 °C in a mixture of sugarcane bagasse/wheat bran (7:3 w/w). Respiration gases were sampled at the top of the column (end-do-end mode) and in three other alternatives of sampling gases. Axial temperature profiles were obtained during the experiments and axial moisture contents and endoglucanase activities were determined after cultivation. The results showed that sampling gases at low flow rate produced similar results to the end-to-end operation. On the other hand, when part of the bed was subjected to inefficient aeration, the process was strongly affected in terms of accumulated gas concentrations and temperature. From the results, it was possible to infer some aspects of the microbial respiratory activity, which will be of help to SSC packed-bed bioreactor developers.
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spelling Automatic system for monitoring gaseous concentration in a packed-bed solid-state cultivation bioreactorMyceliophthora thermophila I-1D3bOn-line gases monitoring, EnzymesPacked bed bioreactorSolid-state cultivationIn this work, the respiration gases were sampled from a packed-bed bioreactor used for solid-state cultivation (SSC) using different gas-sampling procedures. As a case study, Myceliophthora thermophila I-1D3b was cultivated at 45 °C in a mixture of sugarcane bagasse/wheat bran (7:3 w/w). Respiration gases were sampled at the top of the column (end-do-end mode) and in three other alternatives of sampling gases. Axial temperature profiles were obtained during the experiments and axial moisture contents and endoglucanase activities were determined after cultivation. The results showed that sampling gases at low flow rate produced similar results to the end-to-end operation. On the other hand, when part of the bed was subjected to inefficient aeration, the process was strongly affected in terms of accumulated gas concentrations and temperature. From the results, it was possible to infer some aspects of the microbial respiratory activity, which will be of help to SSC packed-bed bioreactor developers.Food Engineering and Technology Department Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP) Cristóvão Colombo 2265 Jardim Nazareth, SPChemical Engineering Department Federal University of São Carlos (UFSCar), Rod. Washington Luís km 235 – SP-310, SPFood Engineering and Technology Department Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP) Cristóvão Colombo 2265 Jardim Nazareth, SPUniversidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)Henrique, João Paulo [UNESP]Casciatori, Fernanda PerpétuaThoméo, João Cláudio [UNESP]2023-03-02T05:22:00Z2023-03-02T05:22:00Z2022-09-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ces.2022.117793Chemical Engineering Science, v. 259.0009-2509http://hdl.handle.net/11449/24196310.1016/j.ces.2022.1177932-s2.0-85132540394Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemical Engineering Scienceinfo:eu-repo/semantics/openAccess2023-03-02T05:22:00Zoai:repositorio.unesp.br:11449/241963Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:13:56.221427Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Automatic system for monitoring gaseous concentration in a packed-bed solid-state cultivation bioreactor
title Automatic system for monitoring gaseous concentration in a packed-bed solid-state cultivation bioreactor
spellingShingle Automatic system for monitoring gaseous concentration in a packed-bed solid-state cultivation bioreactor
Henrique, João Paulo [UNESP]
Myceliophthora thermophila I-1D3b
On-line gases monitoring, Enzymes
Packed bed bioreactor
Solid-state cultivation
title_short Automatic system for monitoring gaseous concentration in a packed-bed solid-state cultivation bioreactor
title_full Automatic system for monitoring gaseous concentration in a packed-bed solid-state cultivation bioreactor
title_fullStr Automatic system for monitoring gaseous concentration in a packed-bed solid-state cultivation bioreactor
title_full_unstemmed Automatic system for monitoring gaseous concentration in a packed-bed solid-state cultivation bioreactor
title_sort Automatic system for monitoring gaseous concentration in a packed-bed solid-state cultivation bioreactor
author Henrique, João Paulo [UNESP]
author_facet Henrique, João Paulo [UNESP]
Casciatori, Fernanda Perpétua
Thoméo, João Cláudio [UNESP]
author_role author
author2 Casciatori, Fernanda Perpétua
Thoméo, João Cláudio [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de São Carlos (UFSCar)
dc.contributor.author.fl_str_mv Henrique, João Paulo [UNESP]
Casciatori, Fernanda Perpétua
Thoméo, João Cláudio [UNESP]
dc.subject.por.fl_str_mv Myceliophthora thermophila I-1D3b
On-line gases monitoring, Enzymes
Packed bed bioreactor
Solid-state cultivation
topic Myceliophthora thermophila I-1D3b
On-line gases monitoring, Enzymes
Packed bed bioreactor
Solid-state cultivation
description In this work, the respiration gases were sampled from a packed-bed bioreactor used for solid-state cultivation (SSC) using different gas-sampling procedures. As a case study, Myceliophthora thermophila I-1D3b was cultivated at 45 °C in a mixture of sugarcane bagasse/wheat bran (7:3 w/w). Respiration gases were sampled at the top of the column (end-do-end mode) and in three other alternatives of sampling gases. Axial temperature profiles were obtained during the experiments and axial moisture contents and endoglucanase activities were determined after cultivation. The results showed that sampling gases at low flow rate produced similar results to the end-to-end operation. On the other hand, when part of the bed was subjected to inefficient aeration, the process was strongly affected in terms of accumulated gas concentrations and temperature. From the results, it was possible to infer some aspects of the microbial respiratory activity, which will be of help to SSC packed-bed bioreactor developers.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-21
2023-03-02T05:22:00Z
2023-03-02T05:22:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.ces.2022.117793
Chemical Engineering Science, v. 259.
0009-2509
http://hdl.handle.net/11449/241963
10.1016/j.ces.2022.117793
2-s2.0-85132540394
url http://dx.doi.org/10.1016/j.ces.2022.117793
http://hdl.handle.net/11449/241963
identifier_str_mv Chemical Engineering Science, v. 259.
0009-2509
10.1016/j.ces.2022.117793
2-s2.0-85132540394
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chemical Engineering Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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