A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00604-017-2443-5 http://hdl.handle.net/11449/179137 |
Resumo: | The dopant bumetanide (BMT) was banned by the World Anti-Doping Agency. The authors describe a voltammetric sensor for the determination of BMT in urine that is based on the use of a glassy carbon electrode modified with reduced graphene oxide (GCE/rGO). The GCE/rGO was prepared by electrodeposition from a solution of GO. The oxidation of BMT occurs best at +0.75 V in the GCE/rGO with an approximately 13-fold gain in the anode current compared to a conventional GCE. The oxidation mechanism was investigated by means of electrolysis, while the analysis of the products was carried out via the LC–MS/MS. Under optimized conditions (scan rate 100 mV s−1; pH 4.0; accumulation time 25 s) and by using linear sweep adsorptive stripping voltammetry, the response covers the 0.26 to 50 μmol L−1 BMT concentration range. The detection limit is 75 nmol L−1. The sensor was successfully applied and validated by LC–MS/MS analysis of spiked urine sample. |
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Repositório Institucional da UNESP |
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A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysisBumetanideDiureticsDopingStripping voltammetryUrine sampleVoltammetric sensorThe dopant bumetanide (BMT) was banned by the World Anti-Doping Agency. The authors describe a voltammetric sensor for the determination of BMT in urine that is based on the use of a glassy carbon electrode modified with reduced graphene oxide (GCE/rGO). The GCE/rGO was prepared by electrodeposition from a solution of GO. The oxidation of BMT occurs best at +0.75 V in the GCE/rGO with an approximately 13-fold gain in the anode current compared to a conventional GCE. The oxidation mechanism was investigated by means of electrolysis, while the analysis of the products was carried out via the LC–MS/MS. Under optimized conditions (scan rate 100 mV s−1; pH 4.0; accumulation time 25 s) and by using linear sweep adsorptive stripping voltammetry, the response covers the 0.26 to 50 μmol L−1 BMT concentration range. The detection limit is 75 nmol L−1. The sensor was successfully applied and validated by LC–MS/MS analysis of spiked urine sample.Universidade Estadual Paulista (Unesp) Instituto de Química Araraquara, Av. Prof. Francisco Degni, 55Universidade Estadual Paulista (Unesp) Instituto de Química Araraquara, Av. Prof. Francisco Degni, 55Universidade Estadual Paulista (Unesp)Hudari, Felipe Fantinato [UNESP]Zanoni, Maria Valnice Boldrin [UNESP]2018-12-11T17:33:54Z2018-12-11T17:33:54Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4117-4124application/pdfhttp://dx.doi.org/10.1007/s00604-017-2443-5Microchimica Acta, v. 184, n. 10, p. 4117-4124, 2017.1436-50730026-3672http://hdl.handle.net/11449/17913710.1007/s00604-017-2443-52-s2.0-850285418132-s2.0-85028541813.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrochimica Acta1,2401,240info:eu-repo/semantics/openAccess2023-10-28T06:08:38Zoai:repositorio.unesp.br:11449/179137Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:15:39.526366Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis |
title |
A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis |
spellingShingle |
A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis Hudari, Felipe Fantinato [UNESP] Bumetanide Diuretics Doping Stripping voltammetry Urine sample Voltammetric sensor |
title_short |
A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis |
title_full |
A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis |
title_fullStr |
A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis |
title_full_unstemmed |
A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis |
title_sort |
A glassy carbon electrode modified with reduced graphene oxide for sensitive determination of bumetanide in urine at levels required for doping analysis |
author |
Hudari, Felipe Fantinato [UNESP] |
author_facet |
Hudari, Felipe Fantinato [UNESP] Zanoni, Maria Valnice Boldrin [UNESP] |
author_role |
author |
author2 |
Zanoni, Maria Valnice Boldrin [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Hudari, Felipe Fantinato [UNESP] Zanoni, Maria Valnice Boldrin [UNESP] |
dc.subject.por.fl_str_mv |
Bumetanide Diuretics Doping Stripping voltammetry Urine sample Voltammetric sensor |
topic |
Bumetanide Diuretics Doping Stripping voltammetry Urine sample Voltammetric sensor |
description |
The dopant bumetanide (BMT) was banned by the World Anti-Doping Agency. The authors describe a voltammetric sensor for the determination of BMT in urine that is based on the use of a glassy carbon electrode modified with reduced graphene oxide (GCE/rGO). The GCE/rGO was prepared by electrodeposition from a solution of GO. The oxidation of BMT occurs best at +0.75 V in the GCE/rGO with an approximately 13-fold gain in the anode current compared to a conventional GCE. The oxidation mechanism was investigated by means of electrolysis, while the analysis of the products was carried out via the LC–MS/MS. Under optimized conditions (scan rate 100 mV s−1; pH 4.0; accumulation time 25 s) and by using linear sweep adsorptive stripping voltammetry, the response covers the 0.26 to 50 μmol L−1 BMT concentration range. The detection limit is 75 nmol L−1. The sensor was successfully applied and validated by LC–MS/MS analysis of spiked urine sample. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-01 2018-12-11T17:33:54Z 2018-12-11T17:33:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00604-017-2443-5 Microchimica Acta, v. 184, n. 10, p. 4117-4124, 2017. 1436-5073 0026-3672 http://hdl.handle.net/11449/179137 10.1007/s00604-017-2443-5 2-s2.0-85028541813 2-s2.0-85028541813.pdf |
url |
http://dx.doi.org/10.1007/s00604-017-2443-5 http://hdl.handle.net/11449/179137 |
identifier_str_mv |
Microchimica Acta, v. 184, n. 10, p. 4117-4124, 2017. 1436-5073 0026-3672 10.1007/s00604-017-2443-5 2-s2.0-85028541813 2-s2.0-85028541813.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microchimica Acta 1,240 1,240 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4117-4124 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128625750310912 |