Análise proteômica do tecido hepático de camundongos com diabetes mellitus induzidos por estreptozotocina e dieta hiperlipídica

• Autor(a) principal: Umemura, Thalissa Yukie Graboski
• Data de Publicação: 2022
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/217494
• topic: Metabolism; Liver; Obesity; Metabolismo; Fígado; Diabetes; Obesidade

Diabetes mellitus affects millions of people, and can cause serious complications such as heart attacks, strokes, kidney failure, among others. Proteomics research provides a clearer metabolic view of mechanistic processes that reveal new ways of treatments, preventions and diagnosing. Diabetes mellitus is a complex and controversial disease. Many metabolic pathways, biological processes and protein alterations are not completely understood and can vary among researches, hence is essential the use of even more precise methods for its analysis. Thus the objective of this study was to evaluate how hepatic protein expression changes, through proteomic analysis of the functioning of mice with or type 2 diabetes mellitus. Thereunto, 18 mice were divided into 3 groups (n=6). The first is formed by animals that received standard diet and SZT injections (CD1), the second are animals that received high fat diet and STZ injections (CD2), and the third group received standard diet (CND), the latter is the control group. The model used was successfully implanted and revealed significant changes in the metabolism of DM2, related to the metabolism of carbohydrates, lipids and proteins. The high-fat diet proved to be a potentiator of the adverse effects of DM2 on the liver. This project also proposed new targets for the understanding of this pathology.