Revealing the Venomous Secrets of the Spider's Web
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1021/acs.jproteome.0c00086 http://hdl.handle.net/11449/200922 |
Resumo: | Orb-weaving spiders use a highly strong, sticky and elastic web to catch their prey. These web properties alone would be enough for the entrapment of prey; however, these spiders may be hiding venomous secrets in the web, which current research is revealing. Here, we provide strong proteotranscriptomic evidence for the presence of toxin/neurotoxin-like proteins, defensins, and proteolytic enzymes on the web silk from Nephila clavipes spider. The results from quantitative-based transcriptomic and proteomic approaches showed that silk-producing glands produce an extensive repertoire of toxin/neurotoxin-like proteins, similar to those already reported in spider venoms. Meanwhile, the insect toxicity results demonstrated that these toxic components can be lethal and/or paralytic chemical weapons used for prey capture on the web, and the presence of fatty acids in the web may be a responsible mechanism opening the way to the web toxins for accessing the interior of prey's body, as shown here. Comparative phylogenomic-level evolutionary analyses revealed orthologous genes among two spider groups, Araneomorphae and Mygalomorphae, and the findings showed protein sequences similar to toxins found in the taxa Scorpiones and Hymenoptera in addition to Araneae. Overall, these data represent a valuable resource to further investigate other spider web toxin systems and also suggest that N. clavipes web is not a passive mechanical trap for prey capture, but it exerts an active role in prey paralysis/killing using a series of neurotoxins. |
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Revealing the Venomous Secrets of the Spider's Webde novo transcriptome assemblyLCMS-based proteomicsneurotoxinsspider silk-producing glandsweb silk toxinsOrb-weaving spiders use a highly strong, sticky and elastic web to catch their prey. These web properties alone would be enough for the entrapment of prey; however, these spiders may be hiding venomous secrets in the web, which current research is revealing. Here, we provide strong proteotranscriptomic evidence for the presence of toxin/neurotoxin-like proteins, defensins, and proteolytic enzymes on the web silk from Nephila clavipes spider. The results from quantitative-based transcriptomic and proteomic approaches showed that silk-producing glands produce an extensive repertoire of toxin/neurotoxin-like proteins, similar to those already reported in spider venoms. Meanwhile, the insect toxicity results demonstrated that these toxic components can be lethal and/or paralytic chemical weapons used for prey capture on the web, and the presence of fatty acids in the web may be a responsible mechanism opening the way to the web toxins for accessing the interior of prey's body, as shown here. Comparative phylogenomic-level evolutionary analyses revealed orthologous genes among two spider groups, Araneomorphae and Mygalomorphae, and the findings showed protein sequences similar to toxins found in the taxa Scorpiones and Hymenoptera in addition to Araneae. Overall, these data represent a valuable resource to further investigate other spider web toxin systems and also suggest that N. clavipes web is not a passive mechanical trap for prey capture, but it exerts an active role in prey paralysis/killing using a series of neurotoxins.Center of the Study of Social Insects Department of General and Applied Biology Institute of Biosciences of Rio Claro University of Saõ Paulo State (UNESP)Department of Pharmaceutical Chemistry University of ViennaDivision of Computational System Biology Department of Microbiology and Ecosystem Science University of ViennaDepartment of Laboratory Medicine and Core Facility Genomics Medical University of ViennaParacelsus Medical UniversityCenter of the Study of Social Insects Department of General and Applied Biology Institute of Biosciences of Rio Claro University of Saõ Paulo State (UNESP)Universidade Estadual Paulista (Unesp)University of ViennaMedical University of ViennaParacelsus Medical UniversityEsteves, Franciele Grego [UNESP]Dos Santos-Pinto, José Roberto Aparecido [UNESP]Ferro, Milene [UNESP]Sialana, Fernando J.Smidak, RomanRares, Lucaciu CalinNussbaumer, ThomasRattei, ThomasBilban, MartinBacci Júnior, Mauricio [UNESP]Lubec, GertPalma, Mario Sergio [UNESP]2020-12-12T02:19:36Z2020-12-12T02:19:36Z2020-08-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3044-3059http://dx.doi.org/10.1021/acs.jproteome.0c00086Journal of Proteome Research, v. 19, n. 8, p. 3044-3059, 2020.1535-39071535-3893http://hdl.handle.net/11449/20092210.1021/acs.jproteome.0c000862-s2.0-85089612846Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Proteome Researchinfo:eu-repo/semantics/openAccess2021-10-23T15:33:31Zoai:repositorio.unesp.br:11449/200922Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T15:33:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Revealing the Venomous Secrets of the Spider's Web |
title |
Revealing the Venomous Secrets of the Spider's Web |
spellingShingle |
Revealing the Venomous Secrets of the Spider's Web Esteves, Franciele Grego [UNESP] de novo transcriptome assembly LCMS-based proteomics neurotoxins spider silk-producing glands web silk toxins |
title_short |
Revealing the Venomous Secrets of the Spider's Web |
title_full |
Revealing the Venomous Secrets of the Spider's Web |
title_fullStr |
Revealing the Venomous Secrets of the Spider's Web |
title_full_unstemmed |
Revealing the Venomous Secrets of the Spider's Web |
title_sort |
Revealing the Venomous Secrets of the Spider's Web |
author |
Esteves, Franciele Grego [UNESP] |
author_facet |
Esteves, Franciele Grego [UNESP] Dos Santos-Pinto, José Roberto Aparecido [UNESP] Ferro, Milene [UNESP] Sialana, Fernando J. Smidak, Roman Rares, Lucaciu Calin Nussbaumer, Thomas Rattei, Thomas Bilban, Martin Bacci Júnior, Mauricio [UNESP] Lubec, Gert Palma, Mario Sergio [UNESP] |
author_role |
author |
author2 |
Dos Santos-Pinto, José Roberto Aparecido [UNESP] Ferro, Milene [UNESP] Sialana, Fernando J. Smidak, Roman Rares, Lucaciu Calin Nussbaumer, Thomas Rattei, Thomas Bilban, Martin Bacci Júnior, Mauricio [UNESP] Lubec, Gert Palma, Mario Sergio [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Vienna Medical University of Vienna Paracelsus Medical University |
dc.contributor.author.fl_str_mv |
Esteves, Franciele Grego [UNESP] Dos Santos-Pinto, José Roberto Aparecido [UNESP] Ferro, Milene [UNESP] Sialana, Fernando J. Smidak, Roman Rares, Lucaciu Calin Nussbaumer, Thomas Rattei, Thomas Bilban, Martin Bacci Júnior, Mauricio [UNESP] Lubec, Gert Palma, Mario Sergio [UNESP] |
dc.subject.por.fl_str_mv |
de novo transcriptome assembly LCMS-based proteomics neurotoxins spider silk-producing glands web silk toxins |
topic |
de novo transcriptome assembly LCMS-based proteomics neurotoxins spider silk-producing glands web silk toxins |
description |
Orb-weaving spiders use a highly strong, sticky and elastic web to catch their prey. These web properties alone would be enough for the entrapment of prey; however, these spiders may be hiding venomous secrets in the web, which current research is revealing. Here, we provide strong proteotranscriptomic evidence for the presence of toxin/neurotoxin-like proteins, defensins, and proteolytic enzymes on the web silk from Nephila clavipes spider. The results from quantitative-based transcriptomic and proteomic approaches showed that silk-producing glands produce an extensive repertoire of toxin/neurotoxin-like proteins, similar to those already reported in spider venoms. Meanwhile, the insect toxicity results demonstrated that these toxic components can be lethal and/or paralytic chemical weapons used for prey capture on the web, and the presence of fatty acids in the web may be a responsible mechanism opening the way to the web toxins for accessing the interior of prey's body, as shown here. Comparative phylogenomic-level evolutionary analyses revealed orthologous genes among two spider groups, Araneomorphae and Mygalomorphae, and the findings showed protein sequences similar to toxins found in the taxa Scorpiones and Hymenoptera in addition to Araneae. Overall, these data represent a valuable resource to further investigate other spider web toxin systems and also suggest that N. clavipes web is not a passive mechanical trap for prey capture, but it exerts an active role in prey paralysis/killing using a series of neurotoxins. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:19:36Z 2020-12-12T02:19:36Z 2020-08-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1021/acs.jproteome.0c00086 Journal of Proteome Research, v. 19, n. 8, p. 3044-3059, 2020. 1535-3907 1535-3893 http://hdl.handle.net/11449/200922 10.1021/acs.jproteome.0c00086 2-s2.0-85089612846 |
url |
http://dx.doi.org/10.1021/acs.jproteome.0c00086 http://hdl.handle.net/11449/200922 |
identifier_str_mv |
Journal of Proteome Research, v. 19, n. 8, p. 3044-3059, 2020. 1535-3907 1535-3893 10.1021/acs.jproteome.0c00086 2-s2.0-85089612846 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Proteome Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3044-3059 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046965883174912 |