Effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs on the production of reactive oxygen species by activated rat neutrophils

Detalhes bibliográficos
Autor(a) principal: Paino, I.M.M. [UNESP]
Data de Publicação: 2005
Outros Autores: Ximenes, V.F. [UNESP], Fonseca, L.M. da [UNESP], Kanegae, M.P.P. [UNESP], Khalil, N.M. [UNESP], Brunetti, Iguatemy Lourenço [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2005000400007
http://hdl.handle.net/11449/7223
Resumo: The release of reactive oxygen specie (ROS) by activated neutrophil is involved in both the antimicrobial and deleterious effects in chronic inflammation. The objective of the present investigation was to determine the effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs (NSAIDs) on the production of ROS by stimulated rat neutrophils. Diclofenac (3.6 µM), indomethacin (12 µM), naproxen (160 µM), piroxicam (13 µM), and tenoxicam (30 µM) were incubated at 37ºC in PBS (10 mM), pH 7.4, for 30 min with rat neutrophils (1 x 10(6) cells/ml) stimulated by phorbol-12-myristate-13-acetate (100 nM). The ROS production was measured by luminol and lucigenin-dependent chemiluminescence. Except for naproxen, NSAIDs reduced ROS production: 58 ± 2% diclofenac, 90 ± 2% indomethacin, 33 ± 3% piroxicam, and 45 ± 6% tenoxicam (N = 6). For the lucigenin assay, naproxen, piroxicam and tenoxicam were ineffective. For indomethacin the inhibition was 52 ± 5% and diclofenac showed amplification in the light emission of 181 ± 60% (N = 6). Using the myeloperoxidase (MPO)/H2O2/luminol system, the effects of NSAIDs on MPO activity were also screened. We found that NSAIDs inhibited both the peroxidation and chlorinating activity of MPO as follows: diclofenac (36 ± 10, 45 ± 3%), indomethacin (97 ± 2, 100 ± 1%), naproxen (56 ± 8, 76 ± 3%), piroxicam (77 ± 5, 99 ± 1%), and tenoxicam (90 ± 2, 100 ± 1%), respectively (N = 3). These results show that therapeutic levels of NSAIDs are able to suppress the oxygen-dependent antimicrobial or oxidative functions of neutrophils by inhibiting the generation of hypochlorous acid.
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spelling Effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs on the production of reactive oxygen species by activated rat neutrophilsNon-steroidal anti-inflammatory drugsNeutrophilsMyeloperoxidaseHypochlorous acidReactive oxygen speciesChemiluminescenceThe release of reactive oxygen specie (ROS) by activated neutrophil is involved in both the antimicrobial and deleterious effects in chronic inflammation. The objective of the present investigation was to determine the effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs (NSAIDs) on the production of ROS by stimulated rat neutrophils. Diclofenac (3.6 µM), indomethacin (12 µM), naproxen (160 µM), piroxicam (13 µM), and tenoxicam (30 µM) were incubated at 37ºC in PBS (10 mM), pH 7.4, for 30 min with rat neutrophils (1 x 10(6) cells/ml) stimulated by phorbol-12-myristate-13-acetate (100 nM). The ROS production was measured by luminol and lucigenin-dependent chemiluminescence. Except for naproxen, NSAIDs reduced ROS production: 58 ± 2% diclofenac, 90 ± 2% indomethacin, 33 ± 3% piroxicam, and 45 ± 6% tenoxicam (N = 6). For the lucigenin assay, naproxen, piroxicam and tenoxicam were ineffective. For indomethacin the inhibition was 52 ± 5% and diclofenac showed amplification in the light emission of 181 ± 60% (N = 6). Using the myeloperoxidase (MPO)/H2O2/luminol system, the effects of NSAIDs on MPO activity were also screened. We found that NSAIDs inhibited both the peroxidation and chlorinating activity of MPO as follows: diclofenac (36 ± 10, 45 ± 3%), indomethacin (97 ± 2, 100 ± 1%), naproxen (56 ± 8, 76 ± 3%), piroxicam (77 ± 5, 99 ± 1%), and tenoxicam (90 ± 2, 100 ± 1%), respectively (N = 3). These results show that therapeutic levels of NSAIDs are able to suppress the oxygen-dependent antimicrobial or oxidative functions of neutrophils by inhibiting the generation of hypochlorous acid.Universidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Departamento de Análises ClínicasUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Departamento de Análises ClínicasAssociação Brasileira de Divulgação Científica (ABRADIC)Universidade Estadual Paulista (Unesp)Paino, I.M.M. [UNESP]Ximenes, V.F. [UNESP]Fonseca, L.M. da [UNESP]Kanegae, M.P.P. [UNESP]Khalil, N.M. [UNESP]Brunetti, Iguatemy Lourenço [UNESP]2014-05-20T13:23:45Z2014-05-20T13:23:45Z2005-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article543-551application/pdfhttp://dx.doi.org/10.1590/S0100-879X2005000400007Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 4, p. 543-551, 2005.0100-879Xhttp://hdl.handle.net/11449/722310.1590/S0100-879X2005000400007S0100-879X2005000400007S0100-879X2005000400007.pdf4419635633356792SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Research1.492info:eu-repo/semantics/openAccess2024-06-21T15:18:34Zoai:repositorio.unesp.br:11449/7223Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:49:29.321374Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs on the production of reactive oxygen species by activated rat neutrophils
title Effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs on the production of reactive oxygen species by activated rat neutrophils
spellingShingle Effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs on the production of reactive oxygen species by activated rat neutrophils
Paino, I.M.M. [UNESP]
Non-steroidal anti-inflammatory drugs
Neutrophils
Myeloperoxidase
Hypochlorous acid
Reactive oxygen species
Chemiluminescence
title_short Effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs on the production of reactive oxygen species by activated rat neutrophils
title_full Effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs on the production of reactive oxygen species by activated rat neutrophils
title_fullStr Effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs on the production of reactive oxygen species by activated rat neutrophils
title_full_unstemmed Effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs on the production of reactive oxygen species by activated rat neutrophils
title_sort Effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs on the production of reactive oxygen species by activated rat neutrophils
author Paino, I.M.M. [UNESP]
author_facet Paino, I.M.M. [UNESP]
Ximenes, V.F. [UNESP]
Fonseca, L.M. da [UNESP]
Kanegae, M.P.P. [UNESP]
Khalil, N.M. [UNESP]
Brunetti, Iguatemy Lourenço [UNESP]
author_role author
author2 Ximenes, V.F. [UNESP]
Fonseca, L.M. da [UNESP]
Kanegae, M.P.P. [UNESP]
Khalil, N.M. [UNESP]
Brunetti, Iguatemy Lourenço [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Paino, I.M.M. [UNESP]
Ximenes, V.F. [UNESP]
Fonseca, L.M. da [UNESP]
Kanegae, M.P.P. [UNESP]
Khalil, N.M. [UNESP]
Brunetti, Iguatemy Lourenço [UNESP]
dc.subject.por.fl_str_mv Non-steroidal anti-inflammatory drugs
Neutrophils
Myeloperoxidase
Hypochlorous acid
Reactive oxygen species
Chemiluminescence
topic Non-steroidal anti-inflammatory drugs
Neutrophils
Myeloperoxidase
Hypochlorous acid
Reactive oxygen species
Chemiluminescence
description The release of reactive oxygen specie (ROS) by activated neutrophil is involved in both the antimicrobial and deleterious effects in chronic inflammation. The objective of the present investigation was to determine the effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs (NSAIDs) on the production of ROS by stimulated rat neutrophils. Diclofenac (3.6 µM), indomethacin (12 µM), naproxen (160 µM), piroxicam (13 µM), and tenoxicam (30 µM) were incubated at 37ºC in PBS (10 mM), pH 7.4, for 30 min with rat neutrophils (1 x 10(6) cells/ml) stimulated by phorbol-12-myristate-13-acetate (100 nM). The ROS production was measured by luminol and lucigenin-dependent chemiluminescence. Except for naproxen, NSAIDs reduced ROS production: 58 ± 2% diclofenac, 90 ± 2% indomethacin, 33 ± 3% piroxicam, and 45 ± 6% tenoxicam (N = 6). For the lucigenin assay, naproxen, piroxicam and tenoxicam were ineffective. For indomethacin the inhibition was 52 ± 5% and diclofenac showed amplification in the light emission of 181 ± 60% (N = 6). Using the myeloperoxidase (MPO)/H2O2/luminol system, the effects of NSAIDs on MPO activity were also screened. We found that NSAIDs inhibited both the peroxidation and chlorinating activity of MPO as follows: diclofenac (36 ± 10, 45 ± 3%), indomethacin (97 ± 2, 100 ± 1%), naproxen (56 ± 8, 76 ± 3%), piroxicam (77 ± 5, 99 ± 1%), and tenoxicam (90 ± 2, 100 ± 1%), respectively (N = 3). These results show that therapeutic levels of NSAIDs are able to suppress the oxygen-dependent antimicrobial or oxidative functions of neutrophils by inhibiting the generation of hypochlorous acid.
publishDate 2005
dc.date.none.fl_str_mv 2005-04-01
2014-05-20T13:23:45Z
2014-05-20T13:23:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2005000400007
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 4, p. 543-551, 2005.
0100-879X
http://hdl.handle.net/11449/7223
10.1590/S0100-879X2005000400007
S0100-879X2005000400007
S0100-879X2005000400007.pdf
4419635633356792
url http://dx.doi.org/10.1590/S0100-879X2005000400007
http://hdl.handle.net/11449/7223
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 38, n. 4, p. 543-551, 2005.
0100-879X
10.1590/S0100-879X2005000400007
S0100-879X2005000400007
S0100-879X2005000400007.pdf
4419635633356792
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
1.492
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 543-551
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica (ABRADIC)
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica (ABRADIC)
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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