Estudo do potencial cariogênicoin vitro de espécies de bifidobactérias e lactobacilos

• Autor(a) principal: Santos, Vanessa Rodrigues dos [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155311; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-05-03/000881552.pdf
• topic: Bifidobacterium; Lactobacillus; Cárie dentária; Desmineralização do dente

The bacteria considered more cariogenic are mutans streptococci, specially Streptococcus mutans and Streptococcus sobrinus. However, other bacterial species, such as Lactobacillus and Bifidobacterium have been also detected in dental caries lesions. Thus, the aim of this study was to verify the ability of species of Bifidobacterium and Lactobacillus associated with mutans streptococci in producing insoluble extracellular polysaccharides (iEPS) and demineralizing dental enamel. Bacterial species used were: Bifidobacterium animalis (ACTIVIA®), B. longum (ATCC 15707), B. lactis (LMG 18905), B. dentium (ATCC 27678),Lactobacillus acidophilus (ATCC 4356), L. casei (ATCC 393), L. casei shirota (Yakult®) Streptococcus mutans (3VF2) and S. sobrinus (ATCC 27607). Bovine enamel blocks (N=180) were used. After the preparation and selection of bovine enamel blocks (n=180) by means the surface hardness, the blocks were divided in 2 groups: G1: bacterial species combined with S. mutans(3FV2) and G2: these species with S. mutans + S. sobrinus. For each combination, 10 blocks were exposed to the bacterial associations for 7 days. Afterwards, the quantity of iEPS and the cross-sectional hardness or subsurface hardness (ΔKHN) values were determined. Data were submitted to variance analysis (one-way) followed by the Bonferroni's test (p<0.05). The results showed that the combinations of S. mutans+ B. animalis or L. acidophilus or B. dentium produced higher quantify of iEPS (p<0.05) and the combination of S. mutans+ B. animalis led to the highest loss of subsurface hardness (p<0.05). In G2, S. mutans + S.sobrinus+ B. longum produced the highest quantity of iEPS and led to the highest enamel demineralization (p<0.05). It was concluded that B. animalis e B. longum when associated with S. mutans and/or S. mutans/S. sobrinus contributes to iEPS and induced higher enamel demineralization