Microbial nanocellulose adherent to human skin used in electrochemical sensors to detect metal ions and biomarkers in sweat

• Autor(a) principal: Silva, Robson R.
• Data de Publicação: 2020
• Outros Autores: Raymundo-Pereira, Paulo A., Campos, Anderson M., Wilson, Deivy, Otoni, Caio G., Barud, Hernane S., Costa, Carlos A.R., Domeneguetti, Rafael R. [UNESP], Balogh, Debora T., Ribeiro, Sidney J.L. [UNESP], Oliveira Jr., Osvaldo N.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.talanta.2020.121153; http://hdl.handle.net/11449/200421
• topic: Bacterial cellulose; Biosensor; Estradiol; Heavy metals; Uric acid; Wearable electronics

The pursuit of biocompatible, breathable and skin-conformable wearable sensors has predominantly focused on synthetic stretchable hydrophobic polymers. Microbial nanocellulose (MNC) is an exceptional skin-substitute natural polymer routinely used for wound dressing and offers unprecedented potential as substrate for wearable sensors. A versatile strategy for engineering wearable sensing platforms is reported, with sensing units made of screen-printed carbon electrodes (SPCEs) on MNC. As-prepared SPCEs were used to detect the toxic metals cadmium (Cd2+) and lead (Pb2+) with limits of detection of 1.01 and 0.43 μM, respectively, which are sufficient to detect these metal ions in human sweat and urine. SPCEs functionalized through anodic pre-treatments were used for detecting uric acid and 17β-estradiol in artificial sweat, with detection limits of 1.8 μM and 0.58 μM, respectively. The electrochemical treatment created oxygen groups on the carbon surfaces, thus improving wettability and hydrophilicity. MNC was herein exploited as an adhesive-free, yet highly skin-adherent platform for wearable sensing devices that also benefit from the semi-permeable, non-allergenic, and renewable features that make MNC unique within the pool of materials that have been used for such a purpose. Our findings have clear implications for the developments on greener and more biocompatible but still efficient substrates and may pave the route for combining immunosensing devices with drug delivery therapies.