Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes

Detalhes bibliográficos
Autor(a) principal: Silva Santos, Ana Carolina da [UNESP]
Data de Publicação: 2015
Outros Autores: Romeiro, Fernando Gomes [UNESP], Sassaki, Ligia Yukie [UNESP], Rodrigues, Josias [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.gutpathogens.com/content/7/1/2
http://hdl.handle.net/11449/128614
Resumo: Background: Escherichia coli is a normal inhabitant of the gut which upon acquiring virulence factors becomes potentially able to cause diseases. Although Eschericia coli population augments in Crohn's disease (CD), the reason of this proliferation is not yet clear. CD associated Escherichia coli shows features of extraintestinal pathogenic categories (ExPEC), and eventually the ability to invade cultured epithelial cells, a property observed among diarrheagenic Escherichia coli (DEC). In this work, data on the characterization of an Escherichia coli isolate from a CD patient reveal that, besides invasiveness, CD associated Escherichia coli may harbor other typical DEC markers, namely those defining enterohemorragic (EHEC) and enteroaggregative (EAEC) pathotypes.Results: The studied strain, detected both in an ileum biopsy and stools, belonged to the B2 Escherichia coli reference collection (EcoR) phylogroup and harbored the intimin, Shiga cytotoxin 1, and AggR transcriptional activator encoding genes (eae, stx1, aggR, respectively); displayed aggregative adherence to Hep-2 cells and an ability to enter Caco-2 cells four times as high as that of EIEC reference strain and half of invasiveness of AIEC LF82. It was able to enter and replicate in J774 macrophages with invasiveness 85 times as high as that of LF82, but with only one sixth of the intracellular proliferation ability of the later. Extracellular products with cytotoxic activity on Vero cells were detected in strain's cultures. Preliminary analysis indicated similarity of this strain's genome with that of O104:H4/2011C-3493.Methods: Following its isolation from a resected CD patient, the strain was characterized by in vitro adhesion and invasion assays to Hep-2, invasion to Caco-2 cells and to J774 macrophages and tested for the ability to form biofilm and to produce Shiga cytotoxins. PCRs were carried out to identify virulence genetic markers and for EcoR phylogrouping. The strain's genome was sequenced by means of Ion torrent PGM platform.Conclusion: The detection, in a CD patient, of an Escherichia coli combining virulence features of multiple DEC pathotypes seems not only to stress the relevance of Escherichia coli to CD etiopathogenesis but also to indicate the existence of new and potentially more virulent strains putatively associated with this disease.
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spelling Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypesEscherichia coliCrohn's diseaseInvasiveMLSTSerotypeAdherenceVirulenceBackground: Escherichia coli is a normal inhabitant of the gut which upon acquiring virulence factors becomes potentially able to cause diseases. Although Eschericia coli population augments in Crohn's disease (CD), the reason of this proliferation is not yet clear. CD associated Escherichia coli shows features of extraintestinal pathogenic categories (ExPEC), and eventually the ability to invade cultured epithelial cells, a property observed among diarrheagenic Escherichia coli (DEC). In this work, data on the characterization of an Escherichia coli isolate from a CD patient reveal that, besides invasiveness, CD associated Escherichia coli may harbor other typical DEC markers, namely those defining enterohemorragic (EHEC) and enteroaggregative (EAEC) pathotypes.Results: The studied strain, detected both in an ileum biopsy and stools, belonged to the B2 Escherichia coli reference collection (EcoR) phylogroup and harbored the intimin, Shiga cytotoxin 1, and AggR transcriptional activator encoding genes (eae, stx1, aggR, respectively); displayed aggregative adherence to Hep-2 cells and an ability to enter Caco-2 cells four times as high as that of EIEC reference strain and half of invasiveness of AIEC LF82. It was able to enter and replicate in J774 macrophages with invasiveness 85 times as high as that of LF82, but with only one sixth of the intracellular proliferation ability of the later. Extracellular products with cytotoxic activity on Vero cells were detected in strain's cultures. Preliminary analysis indicated similarity of this strain's genome with that of O104:H4/2011C-3493.Methods: Following its isolation from a resected CD patient, the strain was characterized by in vitro adhesion and invasion assays to Hep-2, invasion to Caco-2 cells and to J774 macrophages and tested for the ability to form biofilm and to produce Shiga cytotoxins. PCRs were carried out to identify virulence genetic markers and for EcoR phylogrouping. The strain's genome was sequenced by means of Ion torrent PGM platform.Conclusion: The detection, in a CD patient, of an Escherichia coli combining virulence features of multiple DEC pathotypes seems not only to stress the relevance of Escherichia coli to CD etiopathogenesis but also to indicate the existence of new and potentially more virulent strains putatively associated with this disease.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Medical Bacteriology, Department of Microbiology and Immunology, Institute of Biosciences of the State University of São Paulo (UNESP), Brazil, Distrito de Rubião Junior, Botucatu, CEP 18618-970, SP, BrazilDepartment of Internal Medicine, Botucatu Medical School of the State University of São Paulo (UNESP), Brazil, Distrito de Rubião Junior, Botucatu, CEP 18618-970, SP, BrazilFAPESP: 2013/04475-3Biomed Central LtdUniversidade Estadual Paulista (Unesp)Silva Santos, Ana Carolina da [UNESP]Romeiro, Fernando Gomes [UNESP]Sassaki, Ligia Yukie [UNESP]Rodrigues, Josias [UNESP]2015-10-21T13:11:31Z2015-10-21T13:11:31Z2015-01-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-10application/pdfhttp://www.gutpathogens.com/content/7/1/2Gut Pathogens. London: Biomed Central Ltd, v. 7, p. 1-10, 2015.1757-4749http://hdl.handle.net/11449/12861410.1186/s13099-015-0050-8WOS:000350312300001WOS000350312300001.pdf42114321288164094734747821898178Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGut Pathogens2.8091,066info:eu-repo/semantics/openAccess2024-08-14T17:22:13Zoai:repositorio.unesp.br:11449/128614Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes
title Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes
spellingShingle Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes
Silva Santos, Ana Carolina da [UNESP]
Escherichia coli
Crohn's disease
Invasive
MLST
Serotype
Adherence
Virulence
title_short Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes
title_full Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes
title_fullStr Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes
title_full_unstemmed Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes
title_sort Escherichia coli from Crohn’s disease patient displays virulence features of enteroinvasive (EIEC), enterohemorragic (EHEC), and enteroaggregative (EAEC) pathotypes
author Silva Santos, Ana Carolina da [UNESP]
author_facet Silva Santos, Ana Carolina da [UNESP]
Romeiro, Fernando Gomes [UNESP]
Sassaki, Ligia Yukie [UNESP]
Rodrigues, Josias [UNESP]
author_role author
author2 Romeiro, Fernando Gomes [UNESP]
Sassaki, Ligia Yukie [UNESP]
Rodrigues, Josias [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva Santos, Ana Carolina da [UNESP]
Romeiro, Fernando Gomes [UNESP]
Sassaki, Ligia Yukie [UNESP]
Rodrigues, Josias [UNESP]
dc.subject.por.fl_str_mv Escherichia coli
Crohn's disease
Invasive
MLST
Serotype
Adherence
Virulence
topic Escherichia coli
Crohn's disease
Invasive
MLST
Serotype
Adherence
Virulence
description Background: Escherichia coli is a normal inhabitant of the gut which upon acquiring virulence factors becomes potentially able to cause diseases. Although Eschericia coli population augments in Crohn's disease (CD), the reason of this proliferation is not yet clear. CD associated Escherichia coli shows features of extraintestinal pathogenic categories (ExPEC), and eventually the ability to invade cultured epithelial cells, a property observed among diarrheagenic Escherichia coli (DEC). In this work, data on the characterization of an Escherichia coli isolate from a CD patient reveal that, besides invasiveness, CD associated Escherichia coli may harbor other typical DEC markers, namely those defining enterohemorragic (EHEC) and enteroaggregative (EAEC) pathotypes.Results: The studied strain, detected both in an ileum biopsy and stools, belonged to the B2 Escherichia coli reference collection (EcoR) phylogroup and harbored the intimin, Shiga cytotoxin 1, and AggR transcriptional activator encoding genes (eae, stx1, aggR, respectively); displayed aggregative adherence to Hep-2 cells and an ability to enter Caco-2 cells four times as high as that of EIEC reference strain and half of invasiveness of AIEC LF82. It was able to enter and replicate in J774 macrophages with invasiveness 85 times as high as that of LF82, but with only one sixth of the intracellular proliferation ability of the later. Extracellular products with cytotoxic activity on Vero cells were detected in strain's cultures. Preliminary analysis indicated similarity of this strain's genome with that of O104:H4/2011C-3493.Methods: Following its isolation from a resected CD patient, the strain was characterized by in vitro adhesion and invasion assays to Hep-2, invasion to Caco-2 cells and to J774 macrophages and tested for the ability to form biofilm and to produce Shiga cytotoxins. PCRs were carried out to identify virulence genetic markers and for EcoR phylogrouping. The strain's genome was sequenced by means of Ion torrent PGM platform.Conclusion: The detection, in a CD patient, of an Escherichia coli combining virulence features of multiple DEC pathotypes seems not only to stress the relevance of Escherichia coli to CD etiopathogenesis but also to indicate the existence of new and potentially more virulent strains putatively associated with this disease.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-21T13:11:31Z
2015-10-21T13:11:31Z
2015-01-29
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.gutpathogens.com/content/7/1/2
Gut Pathogens. London: Biomed Central Ltd, v. 7, p. 1-10, 2015.
1757-4749
http://hdl.handle.net/11449/128614
10.1186/s13099-015-0050-8
WOS:000350312300001
WOS000350312300001.pdf
4211432128816409
4734747821898178
url http://www.gutpathogens.com/content/7/1/2
http://hdl.handle.net/11449/128614
identifier_str_mv Gut Pathogens. London: Biomed Central Ltd, v. 7, p. 1-10, 2015.
1757-4749
10.1186/s13099-015-0050-8
WOS:000350312300001
WOS000350312300001.pdf
4211432128816409
4734747821898178
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gut Pathogens
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application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
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