Could a B-1 Cell Derived Phagocyte Be One of the Peritoneal Macrophages during LPS-Driven Inflammation?
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0034570 http://hdl.handle.net/11449/197301 |
Resumo: | The inflammatory response is driven by signals that recruit and elicit immune cells to areas of tissue damage or infection. The concept of a mononuclear phagocyte system postulates that monocytes circulating in the bloodstream are recruited to inflamed tissues where they give rise to macrophages. A recent publication demonstrated that the large increase in the macrophages observed during infection was the result of the multiplication of these cells rather than the recruitment of blood monocytes. We demonstrated previously that B-1 cells undergo differentiation to acquire a mononuclear phagocyte phenotype in vitro (B-1CDP), and we propose that B-1 cells could be an alternative origin for peritoneal macrophages. A number of recent studies that describe the phagocytic and microbicidal activity of B-1 cells in vitro and in vivo support this hypothesis. Based on these findings, we further investigated the differentiation of B-1 cells into phagocytes in vivo in response to LPS-induced inflammation. Therefore, we investigated the role of B-1 cells in the composition of the peritoneal macrophage population after LPS stimulation using osteopetrotic mice, BALB/Xid mice and the depletion of monocytes/macrophages by clodronate treatment. We show that peritoneal macrophages appear in op/op((-/-)) mice after LPS stimulation and exhibit the same Ig gene rearrangement (VH11) that is often found in B-1 cells. These results strongly suggest that op/op((-/-)) peritoneal macrophages are B-1CDP. Similarly, the LPS-induced increase in the macrophage population was observed even following monocyte/macrophage depletion by clodronate. After monocyte/macrophage depletion by clodronate, LPS-elicited macrophages were observed in BALB/Xid mice only following the transfer of B-1 cells. Based on these data, we confirmed that B-1 cell differentiation into phagocytes also occurs in vivo. In conclusion, the results strongly suggest that B-1 cell derived phagocytes are a component of the LPS-elicited peritoneal macrophage population. |
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spelling |
Could a B-1 Cell Derived Phagocyte Be One of the Peritoneal Macrophages during LPS-Driven Inflammation?The inflammatory response is driven by signals that recruit and elicit immune cells to areas of tissue damage or infection. The concept of a mononuclear phagocyte system postulates that monocytes circulating in the bloodstream are recruited to inflamed tissues where they give rise to macrophages. A recent publication demonstrated that the large increase in the macrophages observed during infection was the result of the multiplication of these cells rather than the recruitment of blood monocytes. We demonstrated previously that B-1 cells undergo differentiation to acquire a mononuclear phagocyte phenotype in vitro (B-1CDP), and we propose that B-1 cells could be an alternative origin for peritoneal macrophages. A number of recent studies that describe the phagocytic and microbicidal activity of B-1 cells in vitro and in vivo support this hypothesis. Based on these findings, we further investigated the differentiation of B-1 cells into phagocytes in vivo in response to LPS-induced inflammation. Therefore, we investigated the role of B-1 cells in the composition of the peritoneal macrophage population after LPS stimulation using osteopetrotic mice, BALB/Xid mice and the depletion of monocytes/macrophages by clodronate treatment. We show that peritoneal macrophages appear in op/op((-/-)) mice after LPS stimulation and exhibit the same Ig gene rearrangement (VH11) that is often found in B-1 cells. These results strongly suggest that op/op((-/-)) peritoneal macrophages are B-1CDP. Similarly, the LPS-induced increase in the macrophage population was observed even following monocyte/macrophage depletion by clodronate. After monocyte/macrophage depletion by clodronate, LPS-elicited macrophages were observed in BALB/Xid mice only following the transfer of B-1 cells. Based on these data, we confirmed that B-1 cell differentiation into phagocytes also occurs in vivo. In conclusion, the results strongly suggest that B-1 cell derived phagocytes are a component of the LPS-elicited peritoneal macrophage population.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Fed Sao Paulo, UNIFESP, Discipline Immunol, Dept Microbiol Immunol & Parasitol, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Biophys, Sao Paulo, BrazilUniv Estadual Paulista, Lab Mol & Cellular Biol, UNIP, Sao Paulo, BrazilUniv Estadual Paulista, Lab Mol & Cellular Biol, UNIP, Sao Paulo, BrazilFAPESP: 2002/01354-6FAPESP: 2008/55526-9Public Library ScienceUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Popi, Ana FlaviaOsugui, LikaPerez, Katia ReginaLongo-Maugeri, Ieda MariaMariano, Mario [UNESP]2020-12-10T20:12:39Z2020-12-10T20:12:39Z2012-03-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.1371/journal.pone.0034570Plos One. San Francisco: Public Library Science, v. 7, n. 3, 12 p., 2012.1932-6203http://hdl.handle.net/11449/19730110.1371/journal.pone.0034570WOS:000305339100169Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos Oneinfo:eu-repo/semantics/openAccess2021-10-23T12:31:49Zoai:repositorio.unesp.br:11449/197301Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:11:17.523444Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Could a B-1 Cell Derived Phagocyte Be One of the Peritoneal Macrophages during LPS-Driven Inflammation? |
title |
Could a B-1 Cell Derived Phagocyte Be One of the Peritoneal Macrophages during LPS-Driven Inflammation? |
spellingShingle |
Could a B-1 Cell Derived Phagocyte Be One of the Peritoneal Macrophages during LPS-Driven Inflammation? Popi, Ana Flavia |
title_short |
Could a B-1 Cell Derived Phagocyte Be One of the Peritoneal Macrophages during LPS-Driven Inflammation? |
title_full |
Could a B-1 Cell Derived Phagocyte Be One of the Peritoneal Macrophages during LPS-Driven Inflammation? |
title_fullStr |
Could a B-1 Cell Derived Phagocyte Be One of the Peritoneal Macrophages during LPS-Driven Inflammation? |
title_full_unstemmed |
Could a B-1 Cell Derived Phagocyte Be One of the Peritoneal Macrophages during LPS-Driven Inflammation? |
title_sort |
Could a B-1 Cell Derived Phagocyte Be One of the Peritoneal Macrophages during LPS-Driven Inflammation? |
author |
Popi, Ana Flavia |
author_facet |
Popi, Ana Flavia Osugui, Lika Perez, Katia Regina Longo-Maugeri, Ieda Maria Mariano, Mario [UNESP] |
author_role |
author |
author2 |
Osugui, Lika Perez, Katia Regina Longo-Maugeri, Ieda Maria Mariano, Mario [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Popi, Ana Flavia Osugui, Lika Perez, Katia Regina Longo-Maugeri, Ieda Maria Mariano, Mario [UNESP] |
description |
The inflammatory response is driven by signals that recruit and elicit immune cells to areas of tissue damage or infection. The concept of a mononuclear phagocyte system postulates that monocytes circulating in the bloodstream are recruited to inflamed tissues where they give rise to macrophages. A recent publication demonstrated that the large increase in the macrophages observed during infection was the result of the multiplication of these cells rather than the recruitment of blood monocytes. We demonstrated previously that B-1 cells undergo differentiation to acquire a mononuclear phagocyte phenotype in vitro (B-1CDP), and we propose that B-1 cells could be an alternative origin for peritoneal macrophages. A number of recent studies that describe the phagocytic and microbicidal activity of B-1 cells in vitro and in vivo support this hypothesis. Based on these findings, we further investigated the differentiation of B-1 cells into phagocytes in vivo in response to LPS-induced inflammation. Therefore, we investigated the role of B-1 cells in the composition of the peritoneal macrophage population after LPS stimulation using osteopetrotic mice, BALB/Xid mice and the depletion of monocytes/macrophages by clodronate treatment. We show that peritoneal macrophages appear in op/op((-/-)) mice after LPS stimulation and exhibit the same Ig gene rearrangement (VH11) that is often found in B-1 cells. These results strongly suggest that op/op((-/-)) peritoneal macrophages are B-1CDP. Similarly, the LPS-induced increase in the macrophage population was observed even following monocyte/macrophage depletion by clodronate. After monocyte/macrophage depletion by clodronate, LPS-elicited macrophages were observed in BALB/Xid mice only following the transfer of B-1 cells. Based on these data, we confirmed that B-1 cell differentiation into phagocytes also occurs in vivo. In conclusion, the results strongly suggest that B-1 cell derived phagocytes are a component of the LPS-elicited peritoneal macrophage population. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-03-30 2020-12-10T20:12:39Z 2020-12-10T20:12:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0034570 Plos One. San Francisco: Public Library Science, v. 7, n. 3, 12 p., 2012. 1932-6203 http://hdl.handle.net/11449/197301 10.1371/journal.pone.0034570 WOS:000305339100169 |
url |
http://dx.doi.org/10.1371/journal.pone.0034570 http://hdl.handle.net/11449/197301 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 7, n. 3, 12 p., 2012. 1932-6203 10.1371/journal.pone.0034570 WOS:000305339100169 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128329023225856 |