Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vaccine.2018.03.065 http://hdl.handle.net/11449/179751 |
Resumo: | Avian infectious bronchitis virus (IBV) is one of the most important viral diseases of poultry. The mucosa of upper respiratory tract, specially the trachea, is the primary replication site for this virus. However, conventional inactivate IBV vaccines usually elicit reduced mucosal immune responses and local protection. Thus, an inactivated IBV vaccine containing BR-I genotype strain encapsulated in chitosan nanoparticles (IBV-CS) was produced by ionic gelation method to be administered by oculo-nasal route to chickens. IBV-CS vaccine administered alone resulted in markedly mucosal immune responses, characterized by high levels of anti-IBV IgA isotype antibodies and IFNγ gene expression at 1dpi. The association of live attenuated Massachusetts IBV and IBV-CS vaccine also induced strong mucosal immune responses, though a switch from IgA isotype to IgG was observed, and IFNγ gene expression peak was late (at 5 dpi). Efficacy of IBV-CS was evaluated by tracheal ciliostasis analysis, histopathology examination, and viral load determination in the trachea and kidney. The results indicated that IBV-CS vaccine administered alone or associated with a live attenuated heterologous vaccine induced both humoral and cell-mediated immune responses at the primary site of viral replication, and provided an effective protection against IBV infection at local (trachea) and systemic (kidney) sites. |
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Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challengeAvian infectious bronchitis virusBR-I genotypeChitosan nanoparticlesInactivated vaccineMucosal immune responsesAvian infectious bronchitis virus (IBV) is one of the most important viral diseases of poultry. The mucosa of upper respiratory tract, specially the trachea, is the primary replication site for this virus. However, conventional inactivate IBV vaccines usually elicit reduced mucosal immune responses and local protection. Thus, an inactivated IBV vaccine containing BR-I genotype strain encapsulated in chitosan nanoparticles (IBV-CS) was produced by ionic gelation method to be administered by oculo-nasal route to chickens. IBV-CS vaccine administered alone resulted in markedly mucosal immune responses, characterized by high levels of anti-IBV IgA isotype antibodies and IFNγ gene expression at 1dpi. The association of live attenuated Massachusetts IBV and IBV-CS vaccine also induced strong mucosal immune responses, though a switch from IgA isotype to IgG was observed, and IFNγ gene expression peak was late (at 5 dpi). Efficacy of IBV-CS was evaluated by tracheal ciliostasis analysis, histopathology examination, and viral load determination in the trachea and kidney. The results indicated that IBV-CS vaccine administered alone or associated with a live attenuated heterologous vaccine induced both humoral and cell-mediated immune responses at the primary site of viral replication, and provided an effective protection against IBV infection at local (trachea) and systemic (kidney) sites.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castelane, S/N – Vila Industrial, JaboticabalEmbrapa Pecuária Sudeste Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Rodovia Washington Luiz, Km 234 s/n – Fazenda Canchim, São CarlosFaculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo (USP), Avenida Bandeirantes, 3.900 – Monte Alegre, Ribeirão PretoFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castelane, S/N – Vila Industrial, JaboticabalCNPq: 140100/2015-6Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade de São Paulo (USP)Lopes, Priscila Diniz [UNESP]Okino, Cintia HiromiFernando, Filipe Santos [UNESP]Pavani, Caren [UNESP]Casagrande, Viviane Mariguela [UNESP]Lopez, Renata F.V.Montassier, Maria de Fátima Silva [UNESP]Montassier, Helio José [UNESP]2018-12-11T17:36:36Z2018-12-11T17:36:36Z2018-05-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2630-2636application/pdfhttp://dx.doi.org/10.1016/j.vaccine.2018.03.065Vaccine, v. 36, n. 19, p. 2630-2636, 2018.1873-25180264-410Xhttp://hdl.handle.net/11449/17975110.1016/j.vaccine.2018.03.0652-s2.0-850451240962-s2.0-85045124096.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVaccineinfo:eu-repo/semantics/openAccess2024-06-07T13:01:27Zoai:repositorio.unesp.br:11449/179751Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:26:09.537274Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge |
title |
Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge |
spellingShingle |
Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge Lopes, Priscila Diniz [UNESP] Avian infectious bronchitis virus BR-I genotype Chitosan nanoparticles Inactivated vaccine Mucosal immune responses |
title_short |
Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge |
title_full |
Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge |
title_fullStr |
Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge |
title_full_unstemmed |
Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge |
title_sort |
Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge |
author |
Lopes, Priscila Diniz [UNESP] |
author_facet |
Lopes, Priscila Diniz [UNESP] Okino, Cintia Hiromi Fernando, Filipe Santos [UNESP] Pavani, Caren [UNESP] Casagrande, Viviane Mariguela [UNESP] Lopez, Renata F.V. Montassier, Maria de Fátima Silva [UNESP] Montassier, Helio José [UNESP] |
author_role |
author |
author2 |
Okino, Cintia Hiromi Fernando, Filipe Santos [UNESP] Pavani, Caren [UNESP] Casagrande, Viviane Mariguela [UNESP] Lopez, Renata F.V. Montassier, Maria de Fátima Silva [UNESP] Montassier, Helio José [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Lopes, Priscila Diniz [UNESP] Okino, Cintia Hiromi Fernando, Filipe Santos [UNESP] Pavani, Caren [UNESP] Casagrande, Viviane Mariguela [UNESP] Lopez, Renata F.V. Montassier, Maria de Fátima Silva [UNESP] Montassier, Helio José [UNESP] |
dc.subject.por.fl_str_mv |
Avian infectious bronchitis virus BR-I genotype Chitosan nanoparticles Inactivated vaccine Mucosal immune responses |
topic |
Avian infectious bronchitis virus BR-I genotype Chitosan nanoparticles Inactivated vaccine Mucosal immune responses |
description |
Avian infectious bronchitis virus (IBV) is one of the most important viral diseases of poultry. The mucosa of upper respiratory tract, specially the trachea, is the primary replication site for this virus. However, conventional inactivate IBV vaccines usually elicit reduced mucosal immune responses and local protection. Thus, an inactivated IBV vaccine containing BR-I genotype strain encapsulated in chitosan nanoparticles (IBV-CS) was produced by ionic gelation method to be administered by oculo-nasal route to chickens. IBV-CS vaccine administered alone resulted in markedly mucosal immune responses, characterized by high levels of anti-IBV IgA isotype antibodies and IFNγ gene expression at 1dpi. The association of live attenuated Massachusetts IBV and IBV-CS vaccine also induced strong mucosal immune responses, though a switch from IgA isotype to IgG was observed, and IFNγ gene expression peak was late (at 5 dpi). Efficacy of IBV-CS was evaluated by tracheal ciliostasis analysis, histopathology examination, and viral load determination in the trachea and kidney. The results indicated that IBV-CS vaccine administered alone or associated with a live attenuated heterologous vaccine induced both humoral and cell-mediated immune responses at the primary site of viral replication, and provided an effective protection against IBV infection at local (trachea) and systemic (kidney) sites. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:36:36Z 2018-12-11T17:36:36Z 2018-05-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vaccine.2018.03.065 Vaccine, v. 36, n. 19, p. 2630-2636, 2018. 1873-2518 0264-410X http://hdl.handle.net/11449/179751 10.1016/j.vaccine.2018.03.065 2-s2.0-85045124096 2-s2.0-85045124096.pdf |
url |
http://dx.doi.org/10.1016/j.vaccine.2018.03.065 http://hdl.handle.net/11449/179751 |
identifier_str_mv |
Vaccine, v. 36, n. 19, p. 2630-2636, 2018. 1873-2518 0264-410X 10.1016/j.vaccine.2018.03.065 2-s2.0-85045124096 2-s2.0-85045124096.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vaccine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2630-2636 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128359528398848 |