Short-interfering RNAs as antivirals against rabies
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Brazilian Journal of Infectious Diseases |
| Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200011 |
Resumo: | This study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immunofluorescence staining showed a 5-time decrease in virus titer when compared to a non-treated plate, showing a promising new approach to the development of antivirals for rabies treatment. |
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Brazilian Journal of Infectious Diseases |
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Short-interfering RNAs as antivirals against rabiessiRNArabiesantiviralThis study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immunofluorescence staining showed a 5-time decrease in virus titer when compared to a non-treated plate, showing a promising new approach to the development of antivirals for rabies treatment.Brazilian Society of Infectious Diseases2007-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200011Brazilian Journal of Infectious Diseases v.11 n.2 2007reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1590/S1413-86702007000200011info:eu-repo/semantics/openAccessBrandão,Paulo EduardoCastilho,Juliana GaleraFahl,WillianCarnieli Jr.,PedroOliveira,Rafael de NovaesMacedo,Carla IsabelCarrieri,Maria LuizaKotait,Ivaneteeng2007-06-27T00:00:00Zoai:scielo:S1413-86702007000200011Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2007-06-27T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
| dc.title.none.fl_str_mv |
Short-interfering RNAs as antivirals against rabies |
| title |
Short-interfering RNAs as antivirals against rabies |
| spellingShingle |
Short-interfering RNAs as antivirals against rabies Brandão,Paulo Eduardo siRNA rabies antiviral |
| title_short |
Short-interfering RNAs as antivirals against rabies |
| title_full |
Short-interfering RNAs as antivirals against rabies |
| title_fullStr |
Short-interfering RNAs as antivirals against rabies |
| title_full_unstemmed |
Short-interfering RNAs as antivirals against rabies |
| title_sort |
Short-interfering RNAs as antivirals against rabies |
| author |
Brandão,Paulo Eduardo |
| author_facet |
Brandão,Paulo Eduardo Castilho,Juliana Galera Fahl,Willian Carnieli Jr.,Pedro Oliveira,Rafael de Novaes Macedo,Carla Isabel Carrieri,Maria Luiza Kotait,Ivanete |
| author_role |
author |
| author2 |
Castilho,Juliana Galera Fahl,Willian Carnieli Jr.,Pedro Oliveira,Rafael de Novaes Macedo,Carla Isabel Carrieri,Maria Luiza Kotait,Ivanete |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Brandão,Paulo Eduardo Castilho,Juliana Galera Fahl,Willian Carnieli Jr.,Pedro Oliveira,Rafael de Novaes Macedo,Carla Isabel Carrieri,Maria Luiza Kotait,Ivanete |
| dc.subject.por.fl_str_mv |
siRNA rabies antiviral |
| topic |
siRNA rabies antiviral |
| description |
This study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immunofluorescence staining showed a 5-time decrease in virus titer when compared to a non-treated plate, showing a promising new approach to the development of antivirals for rabies treatment. |
| publishDate |
2007 |
| dc.date.none.fl_str_mv |
2007-04-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200011 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200011 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.1590/S1413-86702007000200011 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
text/html |
| dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
| publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
| dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.11 n.2 2007 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
| instname_str |
Brazilian Society of Infectious Diseases (BSID) |
| instacron_str |
BSID |
| institution |
BSID |
| reponame_str |
Brazilian Journal of Infectious Diseases |
| collection |
Brazilian Journal of Infectious Diseases |
| repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
| repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
| _version_ |
1754209239821189120 |