Expression and promoter methylation status of two DNA repair genes in leukocytes from patients undergoing propofol or isoflurane anaesthesia

Detalhes bibliográficos
Autor(a) principal: Freire, Cristiana M.M. [UNESP]
Data de Publicação: 2018
Outros Autores: Braz, Mariana G. [UNESP], Marcondes, João Paulo C [UNESP], Arruda, Nayara M. [UNESP], Braz, José Reinaldo C [UNESP], Rainho, Cláudia A. [UNESP], Braz, Leandro G. [UNESP], Salvadori, Daisy M.F. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1093/mutage/gey001
http://hdl.handle.net/11449/180094
Resumo: Despite the widespread use of the anaesthetics propofol (PROP) and isoflurane (ISO), data about their toxicogenomic potential and interference in epigenetic events are unknown. This study evaluated the expression and methylation profile of two important DNA-repair genes (XRCC1 and hOGG1) in 40 patients undergoing elective and minimally invasive surgery (tympanoplasty and septoplasty) under ISO or PROP anaesthesia.The endpoints were examined at three sampling times: before anaesthesia (T0), 2 h after the beginning of anaesthesia (T2) and 24 h after the beginning of surgery (T24). Both gene expressions were assessed by quantitative real-time polymerase chain reaction (qRT-PCR), whereas methylation specific-PCR (MS-PCR) evaluated the DNA methylation patterns. Increased expression of XRCC1 was observed at T2 only in the PROP group. On the other hand, hOGG1 and XRCC1 expressions were decreased at T24 in both groups. There were no statistical significant differences between the two anaesthetics at the respective sampling times. The methylation status of XRCC1 (methylated at T0) and hOGG1 (unmethylated at T0) remained unchanged in the three sampling times. In conclusion, this study showed modulations of hOGG1 and XRCC1 expression especially 1 day after elective surgery in patients undergoing PROP and ISO anaesthesia. However, the data indicated that methylation was not the mechanism by which the genes were regulated. More studies are warranted to further investigate the possible epigenetic mechanisms involved after exposure to anaesthetics.
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spelling Expression and promoter methylation status of two DNA repair genes in leukocytes from patients undergoing propofol or isoflurane anaesthesiaDespite the widespread use of the anaesthetics propofol (PROP) and isoflurane (ISO), data about their toxicogenomic potential and interference in epigenetic events are unknown. This study evaluated the expression and methylation profile of two important DNA-repair genes (XRCC1 and hOGG1) in 40 patients undergoing elective and minimally invasive surgery (tympanoplasty and septoplasty) under ISO or PROP anaesthesia.The endpoints were examined at three sampling times: before anaesthesia (T0), 2 h after the beginning of anaesthesia (T2) and 24 h after the beginning of surgery (T24). Both gene expressions were assessed by quantitative real-time polymerase chain reaction (qRT-PCR), whereas methylation specific-PCR (MS-PCR) evaluated the DNA methylation patterns. Increased expression of XRCC1 was observed at T2 only in the PROP group. On the other hand, hOGG1 and XRCC1 expressions were decreased at T24 in both groups. There were no statistical significant differences between the two anaesthetics at the respective sampling times. The methylation status of XRCC1 (methylated at T0) and hOGG1 (unmethylated at T0) remained unchanged in the three sampling times. In conclusion, this study showed modulations of hOGG1 and XRCC1 expression especially 1 day after elective surgery in patients undergoing PROP and ISO anaesthesia. However, the data indicated that methylation was not the mechanism by which the genes were regulated. More studies are warranted to further investigate the possible epigenetic mechanisms involved after exposure to anaesthetics.UNESP – São Paulo State University Medical School, Professor Mário Rubens G. Montenegro Av.UNESP – São Paulo State University Institute of Biosciences, Prof. Dr. Antonio Celso Wagner Zanin St.UNESP – São Paulo State University Medical School, Professor Mário Rubens G. Montenegro Av.UNESP – São Paulo State University Institute of Biosciences, Prof. Dr. Antonio Celso Wagner Zanin St.Universidade Estadual Paulista (Unesp)Freire, Cristiana M.M. [UNESP]Braz, Mariana G. [UNESP]Marcondes, João Paulo C [UNESP]Arruda, Nayara M. [UNESP]Braz, José Reinaldo C [UNESP]Rainho, Cláudia A. [UNESP]Braz, Leandro G. [UNESP]Salvadori, Daisy M.F. [UNESP]2018-12-11T17:38:07Z2018-12-11T17:38:07Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article147-152application/pdfhttp://dx.doi.org/10.1093/mutage/gey001Mutagenesis, v. 33, n. 2, p. 147-152, 2018.1464-38040267-8357http://hdl.handle.net/11449/18009410.1093/mutage/gey0012-s2.0-850512792742-s2.0-85051279274.pdf88148235451595040000-0002-0285-1162Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMutagenesis0,9160,916info:eu-repo/semantics/openAccess2024-09-03T13:14:54Zoai:repositorio.unesp.br:11449/180094Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:14:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Expression and promoter methylation status of two DNA repair genes in leukocytes from patients undergoing propofol or isoflurane anaesthesia
title Expression and promoter methylation status of two DNA repair genes in leukocytes from patients undergoing propofol or isoflurane anaesthesia
spellingShingle Expression and promoter methylation status of two DNA repair genes in leukocytes from patients undergoing propofol or isoflurane anaesthesia
Freire, Cristiana M.M. [UNESP]
title_short Expression and promoter methylation status of two DNA repair genes in leukocytes from patients undergoing propofol or isoflurane anaesthesia
title_full Expression and promoter methylation status of two DNA repair genes in leukocytes from patients undergoing propofol or isoflurane anaesthesia
title_fullStr Expression and promoter methylation status of two DNA repair genes in leukocytes from patients undergoing propofol or isoflurane anaesthesia
title_full_unstemmed Expression and promoter methylation status of two DNA repair genes in leukocytes from patients undergoing propofol or isoflurane anaesthesia
title_sort Expression and promoter methylation status of two DNA repair genes in leukocytes from patients undergoing propofol or isoflurane anaesthesia
author Freire, Cristiana M.M. [UNESP]
author_facet Freire, Cristiana M.M. [UNESP]
Braz, Mariana G. [UNESP]
Marcondes, João Paulo C [UNESP]
Arruda, Nayara M. [UNESP]
Braz, José Reinaldo C [UNESP]
Rainho, Cláudia A. [UNESP]
Braz, Leandro G. [UNESP]
Salvadori, Daisy M.F. [UNESP]
author_role author
author2 Braz, Mariana G. [UNESP]
Marcondes, João Paulo C [UNESP]
Arruda, Nayara M. [UNESP]
Braz, José Reinaldo C [UNESP]
Rainho, Cláudia A. [UNESP]
Braz, Leandro G. [UNESP]
Salvadori, Daisy M.F. [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Freire, Cristiana M.M. [UNESP]
Braz, Mariana G. [UNESP]
Marcondes, João Paulo C [UNESP]
Arruda, Nayara M. [UNESP]
Braz, José Reinaldo C [UNESP]
Rainho, Cláudia A. [UNESP]
Braz, Leandro G. [UNESP]
Salvadori, Daisy M.F. [UNESP]
description Despite the widespread use of the anaesthetics propofol (PROP) and isoflurane (ISO), data about their toxicogenomic potential and interference in epigenetic events are unknown. This study evaluated the expression and methylation profile of two important DNA-repair genes (XRCC1 and hOGG1) in 40 patients undergoing elective and minimally invasive surgery (tympanoplasty and septoplasty) under ISO or PROP anaesthesia.The endpoints were examined at three sampling times: before anaesthesia (T0), 2 h after the beginning of anaesthesia (T2) and 24 h after the beginning of surgery (T24). Both gene expressions were assessed by quantitative real-time polymerase chain reaction (qRT-PCR), whereas methylation specific-PCR (MS-PCR) evaluated the DNA methylation patterns. Increased expression of XRCC1 was observed at T2 only in the PROP group. On the other hand, hOGG1 and XRCC1 expressions were decreased at T24 in both groups. There were no statistical significant differences between the two anaesthetics at the respective sampling times. The methylation status of XRCC1 (methylated at T0) and hOGG1 (unmethylated at T0) remained unchanged in the three sampling times. In conclusion, this study showed modulations of hOGG1 and XRCC1 expression especially 1 day after elective surgery in patients undergoing PROP and ISO anaesthesia. However, the data indicated that methylation was not the mechanism by which the genes were regulated. More studies are warranted to further investigate the possible epigenetic mechanisms involved after exposure to anaesthetics.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:38:07Z
2018-12-11T17:38:07Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1093/mutage/gey001
Mutagenesis, v. 33, n. 2, p. 147-152, 2018.
1464-3804
0267-8357
http://hdl.handle.net/11449/180094
10.1093/mutage/gey001
2-s2.0-85051279274
2-s2.0-85051279274.pdf
8814823545159504
0000-0002-0285-1162
url http://dx.doi.org/10.1093/mutage/gey001
http://hdl.handle.net/11449/180094
identifier_str_mv Mutagenesis, v. 33, n. 2, p. 147-152, 2018.
1464-3804
0267-8357
10.1093/mutage/gey001
2-s2.0-85051279274
2-s2.0-85051279274.pdf
8814823545159504
0000-0002-0285-1162
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mutagenesis
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 147-152
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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