Soil CO2 emission and soil attributes associated with the microbiota of a sugarcane area in southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-021-87479-2 http://hdl.handle.net/11449/208600 |
Resumo: | The spatial structure of soil CO2 emission (FCO2) and soil attributes are affected by different factors in a highly complex way. In this context, this study aimed to characterize the spatial variability patterns of FCO2 and soil physical, chemical, and microbiological attributes in a sugarcane field area after reform activities. The study was conducted in an Oxisol with the measurement of FCO2, soil temperature (Ts), and soil moisture (Ms) in a regular 90 × 90-m grid with 100 sampling points. Soil samples were collected at each sampling point at a depth of 0–0.20 m to determine soil physical (density, macroporosity, and microporosity), particle size (sand, silt, and clay), and chemical attributes (soil organic matter, pH, P, K, Ca, Mg, Al, H + Al, cation exchange capacity, and base saturation). Geostatistical analyses were performed to assess the spatial variability and map soil attributes. Two regions (R1 and R2) with contrasting emission values were identified after mapping FCO2. The abundance of bacterial 16S rRNA, pmoA, and nifH genes, determined by real-time quantitative PCR (qPCR), enzymatic activity (dehydrogenase, urease, cellulase, and amylase), and microbial biomass carbon were determined in R1 and R2. The mean values of FCO2 (2.91 µmol m−2 s−1), Ts (22.6 °C), and Ms (16.9%) over the 28-day period were similar to those observed in studies also conducted under Oxisols in sugarcane areas and conventional soil tillage. The spatial pattern of FCO2 was similar to that of macropores, air-filled pore space, silt content, soil organic matter, and soil carbon decay constant. No significant difference was observed between R1 and R2 for the copy number of bacterial 16S rRNA and nifH genes, but the results of qPCR for the pmoA gene presented differences (p < 0.01) between regions. The region R1, with the highest FCO2 (2.9 to 4.2 µmol m−2 s−1), showed higher enzymatic activity of dehydrogenase (33.02 µg TPF g−1 dry soil 24 h−1), urease (41.15 µg NH4–N g−1 dry soil 3 h−1), amylase (73.84 µg glucose g−1 dry soil 24 h−1), and microbial biomass carbon (41.35 µg C g−1 soil) than R2, which had the lowest emission (1.9 to 2.7 µmol m−2 s−1). In addition, the soil C/N ratio was higher in R2 (15.43) than in R1 (12.18). The spatial pattern of FCO2 in R1 and R2 may not be directly related to the total amount of the microbial community (bacterial 16S rRNA) in the soil but to the specific function that these microorganisms play regarding soil carbon degradation (pmoA). |
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Soil CO2 emission and soil attributes associated with the microbiota of a sugarcane area in southern BrazilThe spatial structure of soil CO2 emission (FCO2) and soil attributes are affected by different factors in a highly complex way. In this context, this study aimed to characterize the spatial variability patterns of FCO2 and soil physical, chemical, and microbiological attributes in a sugarcane field area after reform activities. The study was conducted in an Oxisol with the measurement of FCO2, soil temperature (Ts), and soil moisture (Ms) in a regular 90 × 90-m grid with 100 sampling points. Soil samples were collected at each sampling point at a depth of 0–0.20 m to determine soil physical (density, macroporosity, and microporosity), particle size (sand, silt, and clay), and chemical attributes (soil organic matter, pH, P, K, Ca, Mg, Al, H + Al, cation exchange capacity, and base saturation). Geostatistical analyses were performed to assess the spatial variability and map soil attributes. Two regions (R1 and R2) with contrasting emission values were identified after mapping FCO2. The abundance of bacterial 16S rRNA, pmoA, and nifH genes, determined by real-time quantitative PCR (qPCR), enzymatic activity (dehydrogenase, urease, cellulase, and amylase), and microbial biomass carbon were determined in R1 and R2. The mean values of FCO2 (2.91 µmol m−2 s−1), Ts (22.6 °C), and Ms (16.9%) over the 28-day period were similar to those observed in studies also conducted under Oxisols in sugarcane areas and conventional soil tillage. The spatial pattern of FCO2 was similar to that of macropores, air-filled pore space, silt content, soil organic matter, and soil carbon decay constant. No significant difference was observed between R1 and R2 for the copy number of bacterial 16S rRNA and nifH genes, but the results of qPCR for the pmoA gene presented differences (p < 0.01) between regions. The region R1, with the highest FCO2 (2.9 to 4.2 µmol m−2 s−1), showed higher enzymatic activity of dehydrogenase (33.02 µg TPF g−1 dry soil 24 h−1), urease (41.15 µg NH4–N g−1 dry soil 3 h−1), amylase (73.84 µg glucose g−1 dry soil 24 h−1), and microbial biomass carbon (41.35 µg C g−1 soil) than R2, which had the lowest emission (1.9 to 2.7 µmol m−2 s−1). In addition, the soil C/N ratio was higher in R2 (15.43) than in R1 (12.18). The spatial pattern of FCO2 in R1 and R2 may not be directly related to the total amount of the microbial community (bacterial 16S rRNA) in the soil but to the specific function that these microorganisms play regarding soil carbon degradation (pmoA).Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian Biorenewables National Laboratory (LNBR) Brazilian Center for Research in Energy and Materials (CNPEM), Rua Giuseppe Maximo Scolfaro 10000Center for Agricultural Sciences University of Marília (UNIMAR), Av. Hygino Muzzy Filho 1001School of Agricultural and Veterinarian Sciences (FCAV) São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/nUniversity of São Paulo (USP) Center of Nuclear Energy in Agriculture (CENA), Av. Centenário 303School of Agricultural and Veterinarian Sciences (FCAV) São Paulo State University (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/nFAPESP: process 2014/03634-3Brazilian Center for Research in Energy and Materials (CNPEM)University of Marília (UNIMAR)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Moitinho, Mara Regina [UNESP]Teixeira, Daniel De BortoliBicalho, Elton da Silva [UNESP]Panosso, Alan Rodrigo [UNESP]Ferraudo, Antonio Sergio [UNESP]Pereira, Gener Tadeu [UNESP]Tsai, Siu MuiBorges, Beatriz Maria FerrariLa Scala Jr, Newton [UNESP]2021-06-25T11:14:49Z2021-06-25T11:14:49Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-021-87479-2Scientific Reports, v. 11, n. 1, 2021.2045-2322http://hdl.handle.net/11449/20860010.1038/s41598-021-87479-22-s2.0-85104488323Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2024-06-06T13:42:23Zoai:repositorio.unesp.br:11449/208600Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:41:42.639804Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Soil CO2 emission and soil attributes associated with the microbiota of a sugarcane area in southern Brazil |
title |
Soil CO2 emission and soil attributes associated with the microbiota of a sugarcane area in southern Brazil |
spellingShingle |
Soil CO2 emission and soil attributes associated with the microbiota of a sugarcane area in southern Brazil Moitinho, Mara Regina [UNESP] |
title_short |
Soil CO2 emission and soil attributes associated with the microbiota of a sugarcane area in southern Brazil |
title_full |
Soil CO2 emission and soil attributes associated with the microbiota of a sugarcane area in southern Brazil |
title_fullStr |
Soil CO2 emission and soil attributes associated with the microbiota of a sugarcane area in southern Brazil |
title_full_unstemmed |
Soil CO2 emission and soil attributes associated with the microbiota of a sugarcane area in southern Brazil |
title_sort |
Soil CO2 emission and soil attributes associated with the microbiota of a sugarcane area in southern Brazil |
author |
Moitinho, Mara Regina [UNESP] |
author_facet |
Moitinho, Mara Regina [UNESP] Teixeira, Daniel De Bortoli Bicalho, Elton da Silva [UNESP] Panosso, Alan Rodrigo [UNESP] Ferraudo, Antonio Sergio [UNESP] Pereira, Gener Tadeu [UNESP] Tsai, Siu Mui Borges, Beatriz Maria Ferrari La Scala Jr, Newton [UNESP] |
author_role |
author |
author2 |
Teixeira, Daniel De Bortoli Bicalho, Elton da Silva [UNESP] Panosso, Alan Rodrigo [UNESP] Ferraudo, Antonio Sergio [UNESP] Pereira, Gener Tadeu [UNESP] Tsai, Siu Mui Borges, Beatriz Maria Ferrari La Scala Jr, Newton [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Brazilian Center for Research in Energy and Materials (CNPEM) University of Marília (UNIMAR) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Moitinho, Mara Regina [UNESP] Teixeira, Daniel De Bortoli Bicalho, Elton da Silva [UNESP] Panosso, Alan Rodrigo [UNESP] Ferraudo, Antonio Sergio [UNESP] Pereira, Gener Tadeu [UNESP] Tsai, Siu Mui Borges, Beatriz Maria Ferrari La Scala Jr, Newton [UNESP] |
description |
The spatial structure of soil CO2 emission (FCO2) and soil attributes are affected by different factors in a highly complex way. In this context, this study aimed to characterize the spatial variability patterns of FCO2 and soil physical, chemical, and microbiological attributes in a sugarcane field area after reform activities. The study was conducted in an Oxisol with the measurement of FCO2, soil temperature (Ts), and soil moisture (Ms) in a regular 90 × 90-m grid with 100 sampling points. Soil samples were collected at each sampling point at a depth of 0–0.20 m to determine soil physical (density, macroporosity, and microporosity), particle size (sand, silt, and clay), and chemical attributes (soil organic matter, pH, P, K, Ca, Mg, Al, H + Al, cation exchange capacity, and base saturation). Geostatistical analyses were performed to assess the spatial variability and map soil attributes. Two regions (R1 and R2) with contrasting emission values were identified after mapping FCO2. The abundance of bacterial 16S rRNA, pmoA, and nifH genes, determined by real-time quantitative PCR (qPCR), enzymatic activity (dehydrogenase, urease, cellulase, and amylase), and microbial biomass carbon were determined in R1 and R2. The mean values of FCO2 (2.91 µmol m−2 s−1), Ts (22.6 °C), and Ms (16.9%) over the 28-day period were similar to those observed in studies also conducted under Oxisols in sugarcane areas and conventional soil tillage. The spatial pattern of FCO2 was similar to that of macropores, air-filled pore space, silt content, soil organic matter, and soil carbon decay constant. No significant difference was observed between R1 and R2 for the copy number of bacterial 16S rRNA and nifH genes, but the results of qPCR for the pmoA gene presented differences (p < 0.01) between regions. The region R1, with the highest FCO2 (2.9 to 4.2 µmol m−2 s−1), showed higher enzymatic activity of dehydrogenase (33.02 µg TPF g−1 dry soil 24 h−1), urease (41.15 µg NH4–N g−1 dry soil 3 h−1), amylase (73.84 µg glucose g−1 dry soil 24 h−1), and microbial biomass carbon (41.35 µg C g−1 soil) than R2, which had the lowest emission (1.9 to 2.7 µmol m−2 s−1). In addition, the soil C/N ratio was higher in R2 (15.43) than in R1 (12.18). The spatial pattern of FCO2 in R1 and R2 may not be directly related to the total amount of the microbial community (bacterial 16S rRNA) in the soil but to the specific function that these microorganisms play regarding soil carbon degradation (pmoA). |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:14:49Z 2021-06-25T11:14:49Z 2021-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-021-87479-2 Scientific Reports, v. 11, n. 1, 2021. 2045-2322 http://hdl.handle.net/11449/208600 10.1038/s41598-021-87479-2 2-s2.0-85104488323 |
url |
http://dx.doi.org/10.1038/s41598-021-87479-2 http://hdl.handle.net/11449/208600 |
identifier_str_mv |
Scientific Reports, v. 11, n. 1, 2021. 2045-2322 10.1038/s41598-021-87479-2 2-s2.0-85104488323 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128551072825344 |